The Action of Divalent Zinc, Cadmium, Mercury, Copper and Lead on the Trans-Root Potential and H+, Efflux of Excised Roots

Kennedy, Colin D.; Gonsalves, F. A. N.

doi:10.1093/jxb/38.5.800

Cited by 95 publications

(43 citation statements)

References 24 publications

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“…In the present experiments, the Hg 2+ -induced membrane depolarization was concentration-dependent and partially reversible. The rapidity and the reversibility of the Hg 2+ -induced depolarization indicate that Hg 2+ may influence the structure and the permeability of plant cell membranes (Kennedy and Gonsalves 1987). Further evidence was provided by the work of Hendrix and Higinbotham (1974), who found that Hg 2+ severely inhibits K + uptake into pea stem while having little effect on respiration.…”

Section: Discussionmentioning

confidence: 99%

Membrane potential differences and viability of grapevine root cells treated with HgCl2

Repka¹,

Fiala²,

Čarná³

et al. 2013

PLANT SOIL ENVIRON

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The effect of mercury (Hg) on the electrophysiological and permeability properties of grapevine adventitious root cells was examined. In short-term experiments, the apical segments of adventitious roots were treated with different concentrations of mercury (0.01 µmol/L to 2 µmol/L HgCl 2 ), and trans-membrane electrical potential differences (E M ) were monitored in root cortical cells, localized in distinct root zones. Based on Hg-induced decrease of E M , we can confirm that the depolarization of the membrane is an instant Hg-response and the extent of E M decrease is not only time-and concentration dependent, but also related to the developmental stage of cells and their localization on root axis. The sensitivity of root cells to Hg declined in the direction of cell division zone > cell elongation zone > absorption zone. Long-term treatment of roots with 2 µmol/L mercury showed that Hg-induced decrease of E M was accompanied by an increase of K + efflux and a decrease of the diffusion potential (E D ) values. Application of fusicoccin (H + -ATPase activator) to the root medium caused an immediate hyperpolarization of the membrane in control and Hg-treated cells. Laser scanning confocal microscopy analysis confirmed that Hg reduced cell viability, which was accompanied with the occurrence of cell death hallmarks, like condensation of protoplasts, nuclei fragmentation and deposition of granular material.

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Section: Discussionmentioning

confidence: 99%

Membrane potential differences and viability of grapevine root cells treated with HgCl2

Repka¹,

Fiala²,

Čarná³

et al. 2013

PLANT SOIL ENVIRON

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“…These observations of anion accumulation by barley roots provide added support for the contention that Zn plays a role in root-cell ion absorption processes in general. Other investigators have also reported that Zn supply affects the accumulation of mineral nutrients by higher plant roots (Loughman et al, 1982;Kumar et al, 1986;Graham et al, 1987;Kennedy and Bonsalves, 1987;Schwartz et al, 1987;Cakmak and Marschner, 1990;Kochian, 1991). Figure 6 shows the effects of increasing Zn supply on the accumulation of water-extractable Na by 17-d-old barley seedlings.…”

Section: Shoot and Root Concentrations Of Mineral Nutrientsmentioning

confidence: 93%

Growth and Nutrient Uptake by Barley (Hordeum vulgare L. cv Herta): Studies Using an N-(2-Hydroxyethyl)ethylenedinitrilotriacetic Acid-Buffered Nutrient Solution Technique (I. Zinc Ion Requirements)

1993

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characterized chelating agents for micronutrient cations. Severa1 applications of chelate buffering used as a means to regulate metal ion availability were discussed by Chaney near or below approximately 3 X 10-" M, barley shoots accumulated higher concentrations of P, Mn, Ca, Mg, and Na, whereas Cu concentrations were reduced. P and M n began to accumulate i n the shoots before differences in dry weights were apparent and provided the earliest index of Zn deficiency. In Zn-deficient roots, concentrations of Ca and Mg increased by 25 to 30%, and those of Fe and M n more than doubled. Zn appears to play a special role in regulating uptake of several mineral nutrients in barley.were below about l o -~l M. The relationship in shoots between symptoms, concentrations of Zn in plant shoots, and disturb- MATERIALS AND METHODS Preparation of Nutrient Solutions

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“…An excess of this metal ion may induce toxicity symptoms such as growth reduction (Barcelo & Poschenrieder, 1990 ;Steffens, 1990), membrane damage (Kennedy & Gonsalves, 1987), and alteration of enzyme activities (Van Assche & Clijsters, 1990).…”

Section: mentioning

confidence: 99%

Effects of cadmium on growth of Helianthus annuus seedlings: physiological aspects

et al. 1999

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Sunflower seedlings (Helianthus annuus hybrid Select) were grown in a complete nutrient solution in the absence or presence of Cd# + (10 and 20 µM). Analyses were performed to establish whether there was a differential effect of Cd# + on mature and young leaves. After 7 d the growth parameters as well as the leaf area had decreased in both mature and young leaves. Accumulation of Cd# + in the roots exceeded that in the shoots. Seedlings treated with Cd# + exhibited reduced contents of chlorophyll and CO # assimilation rate, with a greater decrease in young leaves. The photochemical efficiency of photosystem II (PSII) was not altered by Cd# + treatment in either mature or young leaves, although during steady-state photosynthesis in young leaves there was a significant alteration in the following parameters : quantum yield of electron transport by PSII (Φ PSII ), photochemical quenching (q P ), nonphotochemical quenching (q NP ), and excitation capture efficiency of PSII (Φ exc ).

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The Action of Divalent Zinc, Cadmium, Mercury, Copper and Lead on the Trans-Root Potential and H⁺, Efflux of Excised Roots

Cited by 95 publications

References 24 publications

Membrane potential differences and viability of grapevine root cells treated with HgCl<sub>2</sub>

Membrane potential differences and viability of grapevine root cells treated with HgCl<sub>2</sub>

Growth and Nutrient Uptake by Barley (Hordeum vulgare L. cv Herta): Studies Using an N-(2-Hydroxyethyl)ethylenedinitrilotriacetic Acid-Buffered Nutrient Solution Technique (I. Zinc Ion Requirements)

Effects of cadmium on growth of Helianthus annuus seedlings: physiological aspects

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