The action mode of Thermus aquaticus YT-1 4-α-glucanotransferase and its chimeric enzymes introduced with starch-binding domain on amylose and amylopectin

Analysis of carbohydrates and glycoconjugates by matrix‐assisted laser desorption/ionization mass spectrometry: An update for 2007–2008

“…Novel iso-amylase possessing the properties of 4-α -glucanotransferase Other enzymes acting on sugars 4-α -Glucanotransferase (TA αGT) Thermus aquaticus TOF (DHB) Action mode with starch-binding domain on amylose + amylopectin (Park, et al, 2007c) Chitin oligosaccharide deacetylase Vibrio cholerae TOF, ESI Characterization and genetics (Li, et al, 2007f) Cyclic-β -1,2-glucan synthase Various bacteria TOF (DHB) Mechanism for regulating the size of cyclic periplasmic glucans (Ciocchini, et al, 2007) Glycogen debranching enzyme Porcine liver TOF (DHB) Study of substrate specificity of 4-α-glucanotransferase activity (Watanabe, et al, 2008b) Heparosan synthases (Han, Yoshida, & Uryu, 2007a). In another publication (Han et al, 2007b), the authors report the preparation of further cellobiose-polylysine dendrimers containing 8 and 24 antennae.…”

“…Action mode with starch-binding domain on amylose and amylopectin (Park, et al, 2007c) 4-α -glucanotransferase (TAαGT)…”

Multi-wavelength colorimetric determination of large-ring cyclodextrin content for the cyclization activity of 4-α-glucanotransferase

“…Integration of the peak area before and after the enzymatic treatments gave a ratio of 0.47, which was used as a correction factor to recalculate the actual cyclization activity of 4␣GTase ( Table 2). The final cyclization activity accounted for only 12% of its total activity regarding the amylose degradation, further indicating the infeasibility of using the amylose hydrolytic activity to calculate the cyclization activity towards LR-CD synthesis (Do et al, 2012;Park et al, 2007;Seo et al, 2007). The incapability of distinguishing the linear malto-oligosaccharides from LR-CDs exhibiting a similar DP distribution accounted for a major drawback of the multi-wavelength colorimetric methods shown here.…”

“…Previous studies have widely defined the enzymatic activity of 4-␣-glucanotransferase (4␣GTase, EC 2.4.1.25), an enzyme able to synthesize cyclic glucans, with its starch-degrading capability which can be easily determined by iodine test and the corresponding single wavelength colorimetry (SWC) (Do et al, 2012;Park et al, 2007;Seo et al, 2007). However, this method has two disadvantages.…”

Study of inclusion complexes of cycloamylose with surfactants by isothermal titration calorimetry

“…Amylose and amylopectin exhibit distinct physical properties, indicating that their glucose polymeric structures underlie their functionality. Accordingly, there is increasing interest in engineering glucose polymers with distinct structures and properties to provide novel applications that cannot be achieved with conventional starch (Fujii et al, 2003;Park et al, 2007;Takaha et al, 1998). Large-ring cyclodextrins, also called cycloamylose (CA), can be produced by disproportionating enzyme(s) and have been attracting attention despite existing difficulties in their synthesis, isolation, and purification (Kitamura, Nakatani, Takaha, & Okada, 1999;Machida et al, 2000;Shimada, Handa, Kaneko, & Takada, 1996;Ueda, 2002).…”