THE ACCUMULATION OF RIBONUCLEIC ACID BY A MUTANT OF <i>ESCHERICHIA COLI</i>

Dagley, S.; Turnock, G.; Wild, D. G.

doi:10.1042/bj0880555

Cited by 78 publications

(34 citation statements)

References 31 publications

(26 reference statements)

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“…They agreed to call these particles by their present name: ribosomes. We also investigated ribosomelike particles present in certain "relaxed" mutants of Escherichia coli (37) and in cells treated with chloram phenicol (34,38); but as my own attention was increasingly monopolized by metabolic research, investigations of ribosomes were taken over by col leagues (62,78).…”

Section: Lessons From Biodegrada Nonmentioning

confidence: 99%

Lessons From Biodegradation

Dagley

1987

Annu. Rev. Microbiol.

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Section: Lessons From Biodegrada Nonmentioning

confidence: 99%

Lessons From Biodegradation

Dagley

1987

Annu. Rev. Microbiol.

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“…With RCrCl strains, normal amounts of mRNA and stable RNA (rRNA+tRNA) are produced in conditions of amino acid deprivation (Fleissner & Borek, 1962;Dagley et al, 1963;Turnock & Wild, 1964;Friesen, 1966). In many ways the strain behaves much as RC5tr strains inhibited by high concentrations of chloramphenicol (Friesen, 1966).…”

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confidence: 99%

The control of ribonucleic acid synthesis in bacteria. The synthesis and stability of ribonucleic acids in relaxed and stringent amino acid auxotrophs of Escherichia coli

Gray

Midgley

1972

Biochemical Journal

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The biosynthesis and stability of various RNA fractions was studied in RC(str) and RC(rel) multiple amino acid auxotrophs of Escherichia coli. In conditions of amino acid deprivation, RC(str) mutants were labelled with exogenous nucleotide bases at less than 1% of the rate found in cultures growing normally in supplemented media. Studies by DNA-RNA hybridization and by other methods showed that, during a period of amino acid withdrawal, not more than 60-70% of the labelled RNA formed in RC(str) mutants had the characteristics of mRNA. Evidence was obtained for some degradation of newly formed 16S and 23S rRNA species to heterogeneous material of lower molecular weight. This led to overestimations of the mRNA content of rapidly labelled RNA from such methods as simple examination of sucrose-density-gradient profiles. In RC(rel) strains the absolute and relative rates of synthesis of the various RNA fractions were not greatly affected. However, the stability of about half of the mRNA fraction was increased in RC(rel) strains during amino acid starvation, giving kinetics of mRNA labelling and turnover that were identical with those found in either RC(str) or RC(rel) strains inhibited by high concentrations of chloramphenicol. Coincidence hybridization techniques showed that the mRNA content of amino acid-starved RC(str) auxotrophs was unchanged from that found in normally growing cells. In contrast, RC(rel) strains deprived of amino acids increased their mRNA content about threefold. In such cultures the mRNA content of accumulating newly formed RNA was a constant 16% by wt.

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“…The proportion of translational coding mistakes by leucyl and phenylalanyl transfer RNA was significantly increased if the transfer RNA was hypomethylated during its synthesis (4,27). This approach is not applicable for determining the function of methylated bases in ribosomal RNA since the inhibition of protein synthesis during amino acid starvation necessarily inhibits the maturation of ribonucleoprotein precursors into 30S and 50S subunits (6). Starvation precludes the synthesis of active ribosomes containing hypomethylated RNA until after the restoration of protein synthesis by the addition of methionine.…”

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In Vivo Suppression of Coding Associated with Bacteriophage-Induced S -Adenosylmethionine Hydrolase

Siersma

Lederberg

1970

J Bacteriol

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The methylation of ribosomal and transfer ribonucleic acid (RNA) synthesized after the induction of a hydrolase for S-adenosylmethionine by phage T3 infection is reducible to 50% of the methylation of RNA in uninfected cells. Hypomethylated ribosomal RNA is found in 70S particles that dissociate in 100 ,lM Mg++ to yield only 30S and 50S subunits. By this criterion, the omitted methyl groups apparently are not required for ribosomal maturation or stability. The rate of production of alkaline phosphatase in a phosphatase amber mutant was examined after phage infection in the presence and in the absence of streptomycin to determine the effect on the translation process consequent to S-adenosyl-L-methionine (SAM) hydrolase induction. Significant increases in the rates of phosphatase production were found when ultraviolet-inactivated T3 or streptomycin was added. The effects were cumulative when the cells were treated with both bacteriophage and the drug. Ultraviolet-inactivated T7, a phage closely related to T3 but which does not produce the SAM hydrolase, did not enhance the rate of alkaline phosphatase production. We suggest that the production of SAM hydrolase affects the stability of the translation process by the observed hypomethylation or by mechanisms concerning polyamine metabolism. grown with limiting amounts of methionine, the methylation of RNA is preferentially spared and 398

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THE ACCUMULATION OF RIBONUCLEIC ACID BY A MUTANT OF ESCHERICHIA COLI

Cited by 78 publications

References 31 publications

Lessons From Biodegradation

Lessons From Biodegradation

The control of ribonucleic acid synthesis in bacteria. The synthesis and stability of ribonucleic acids in relaxed and stringent amino acid auxotrophs of Escherichia coli

In Vivo Suppression of Coding Associated with Bacteriophage-Induced S -Adenosylmethionine Hydrolase

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