The Absence of Wasting in Thymectomized Germfree (Axenic) Mice.

Wilson, Robert A.; Sjodin, Kenneth; Bealmear, M.

doi:10.3181/00379727-117-29545

Cited by 37 publications

(8 citation statements)

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“…When animals are deprived of their thymus very early in their development they fail to grow normally; soon they begin to lose weight and exhibit other signs of disease, and they die early. These features are not seen in neonatally thymectomized mice that are raised in a germ free environment (80,81).…”

Section: Role Of the Thymus In Immune Developmentmentioning

confidence: 81%

Role of the Thymus and Other Central Lymphoid Tissues in Immunological Disease

Cooper¹,

Gabrielsen²,

Ra³

1967

Annu. Rev. Med.

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Section: Role Of the Thymus In Immune Developmentmentioning

confidence: 81%

Role of the Thymus and Other Central Lymphoid Tissues in Immunological Disease

Cooper¹,

Gabrielsen²,

Ra³

1967

Annu. Rev. Med.

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“…If wasting disease is a consequence of infection or infestation, or of intoxication from bacterial, viral or fungal flora, as recent studies with germ-free mice suggest, (21,22), the infection or intoxication is preventable and even completely reversible if the mice are given enough lymphoid cells from syngeneic, hemiallogeneic, or even allogeneic donors. It is important that the lymphoid cells administered be cells incapable of destructive graft versus host assault on the thymectomized host.…”

Section: Discussionmentioning

confidence: 99%

Studies on Immunologic Reconstitution of Thymectomized Mice

Yunis

Hilgard

Martínez

et al. 1965

The Journal of Experimental Medicine

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1. Immunologic function, growth, and longevity of neonatally thymectomized mice was restored by intraperitoneal administration of 100 to 400 million syngeneic, hemiallogeneic, or ailogeneic thymus cells from newborn or adult donors. Assays of the graft versus host capabilities of spleen cells from the animals restored with allogeneic cells showed that their immunologically competent cells are of donor histocompatibility characteristics. Such animals accepted skin grafts from mice of the cell donor strain, but rejected skin from a third strain. 2. Similar results were obtained when the neonatally thymectomized animals were treated with 10 to 100 million syngeneic, hemiallogeneic, or allogeneic cells from adult spleen. 3. In one strain combination, C3H recipients and A donors, injected thymus or spleen cells apparently attacked host tissues, since the animals died very early of wasting disease. When this combination was reversed, A strain recipients treated with C3H cells were reconstituted immunologically and physiologically. 4. Syngeneic or allogeneic adult spleen, grafted in the newborn period, reconstituted neonatally thymectomized mice, but all experiments involving grafting of newborn spleen failed. Immunogenetic analysis of the host spleen cells from two allogeneic spleen-grafted animals previously thymectomized showed that the reconstitution was entirely of donor histocompatibility characteristics. 5. Postthymectomy wasting disease was reversed by administration of 200 million adult syngeneic spleen or thymus cells. Immunologic recovery was confirmed by graft versus host assays of the spleens of the recovered animals and by application of allogeneic skin grafts. Some of the animals have been under observation for 42 weeks and appear to be normal. 6. The wasting syndrome in neonatally thymectomized mice was also reversed by injection of 200 million hemiallogeneic or allogeneic spleen cells. 7. Thymus grafts did not reverse wasting disease, whether the donors were adult or newborn, of the same strain or a different one. 8. Spleen, lymph node, and Peyer's patches from representative animals of the reconstituted groups were examined and compared with the tissues of untreated neonatally thymectomized mice and intact animals of the same strain. Tissues of normal cellularity and follicular organization were found in some of the reconstituted animals and also in mice with reversed wasting disease. Extreme deficit of the lymphoid tissues was rare in either group.

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“…Such animals rarely runt or waste (39,73), suggesting that the bacterial flora plays an essential role in the syndrome. Certainly the bacterial, viral or fungal flora could play an important role in development of the entire syndrome described in the rabbits in the present study and in rabbits sub jected to extirpation of the central lymphoid tissue during the newborn period.…”

Section: Discussionmentioning

confidence: 99%