The absence of human papilloma viral DNA sequences in condylomata acuminata

DeLap, Robert J.; Friedman‐Kien, Alvin E.; Rush, Mark G.

doi:10.1016/0042-6822(76)90155-0

Cited by 26 publications

(13 citation statements)

References 16 publications

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“…Viruses of genital warts (condylomata acuminata) have been found by immunological methods to differ from skin warts [Almeida et al, 1969;Almeida and Oriel, 1970;Genner, 1971] as well as by the mole cular hybridization technique [Delap et al, 1976;Orth et al. 1978b], The laryngeal papilloma virus also showed no DNA sequence homology with the skin wart virus [Zur Hausen et al, 1974].…”

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Twenty-One Years of Follow-Up Studies of Familial Epidermodysplasia verruciformis

et al. 1979

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21 years of follow-up study of a family with epidermodysplasia verruciformis (e.v.) have shown that members of one family can be infected with different human papiUomaviruses (HPVs), either HPV 3 or HPV 4, and sometimes with both. The clinical picture resembled disseminated flat warts in cases induced by HPV 3, whereas in those caused by HPV 4 there were flat red or red-brownish plaques and depigmented pityriasis versicolor-like lesions. Malignancies developed only in family members infected with HPV 4, whereas the cases due to HPV 3 ran a more benign and slowly progressive or stationary course. There were also abortive and regressive cases, and the 3 children in whom the wart-like lesions did not recur after removal had an unimpaired cell-mediated immunity (CMI). In all cases of e.v., irrespective of the inducing virus, CMI was low, which seems to be an important factor in the pathogenesis of the disease. Humoral antibodies directed specifically against HPV 3 were present in the majority of the cases, mainly those infected with HPV 3.

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Twenty-One Years of Follow-Up Studies of Familial Epidermodysplasia verruciformis

et al. 1979

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“…Similar calculations for embryonic bursal small circular DNA preparations (where the ratio of mitochondrial to small circular molecules was about 40:1) resulted in an estimate of 0.2 molecules per cell after making the same correction for recovery. It should be noted that the actual total number of small circular DNA molecules per cell (both forms I and II) cannot be obtained from this data, because the preparative procedure is selective for form I molecules; however, if the stability of closed circular DNAs in chicken bursas is similar to that in BSC-1 (3) and HeLa (1) [4][5] weeks, at least in terms of the immunoglobulin isotypes expressed on the cell surface. Some quantitative change in the function of the bursa (rather than a qualitative one) may be responsible for the observed changes in the small circular DNAs isolable from this tissue at these two points in the life cycle of the chicken.…”

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“…For example, such species have been identified .in human (HeLa) (1), mouse (1), and monkey (BSC-1) (2,3) cell lines, Drosophila eggs and cell lines (4), and human warts (5). At present, the most extensive information regarding purified spcDNA relates to the material derived from HeLa (1), Drosophila (4), and BSC-1 (3) cells.…”

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“…It is proposed that these molecules may represent products of immunoglobulin gene rearrangements, which are known to occur during development of B cells (6)(7)(8) 200 embryos yielded 100 g of liver and 7 g of bursa, while 7-10 chickens, [4][5] weeks old, yielded 50 g of liver and 10 g of bursa. To prepare small circular DNA from these tissues, 5-10 g of bursa or 30-40 g of liver was thawed on ice, minced into cold (40C) 0.15 M EDTA at pH 8.0 (8 ml/g of tissue), and homogenized with two strokes of a Kontes Duall 25 homogenizer.…”

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Change in quantity and size distribution of small circular DNAs during development of chicken bursa.

DeLap¹,

Rush²

1978

Proc. Natl. Acad. Sci. U.S.A.

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Small circular DNAs ranging in contour length from 0.06 to 3.5 ;&m have been isolated from bursas of 19.day chicken embryos and 4-to 5-week-old chickens. Small (4), and human warts (5). At present, the most extensive information regarding purified spcDNA relates to the material derived from HeLa (1), Drosophila (4), and BSC-1 (3) cells. Specifically, HeLa spcDNA appears to be predominantly cytoplasmic, present at a level of about 100400 circles per exponentially growing cell, of greater molecular complexity than its average molecular weight, and derived from chromosomal DNA; Drosophila spcDNA appears to be predominantly nuclear, present at a level of about 3-40 circles per cell, and composed of molecules with different sensitivities to restriction endonuclease digestion; BSC-1 spcDNA appears to be present at a level of about 1000-2000 circles per cell, resolvable into relatively distinct size classes by gel electrophoresis, of a greater molecular complexity (in the range of 1 X 108) than its average molecular weight (5 X 105) and derived, at least in part, from chromosomal DNA. It should be noted that no biological function has been assigned to any of these spcDNAs.In this communication we report the isolation and characterization of covalently closed (form I) small circular DNAs from the developing chicken bursa, an organ known to contain large numbers of B lymphocytes undergoing development to a committed state for the production of particular antibodies; in brief, small circular DNAs derived from the bursas of embryonic (19-day) and 4-to 5-week-old chickens differed markedly in both quantity and size distribution. It is proposed that these molecules may represent products of immunoglobulin gene rearrangements, which are known to occur during development of B cells (6)(7)(8) at 19 days (2 days before hatching), and livers and bursas were removed, frozen on dry ice, and stored at -20'C until use. Four-to 5-week-old chickens (White Leghorns, H and N strain, obtained from Truslow) were sacrificed, and organs were removed and frozen in the same way. In general, 200 embryos yielded 100 g of liver and 7 g of bursa, while 7-10 chickens, 4-5 weeks old, yielded 50 g of liver and 10 g of bursa. To prepare small circular DNA from these tissues, 5-10 g of bursa or 30-40 g of liver was thawed on ice, minced into cold (40C) 0.15 M EDTA at pH 8.0 (8 ml/g of tissue), and homogenized with two strokes of a Kontes Duall 25 homogenizer. Sodium dodecyl sulfate (NaDodSO4), 10% wt/vol, was then added to a final concentration of 0.5% and the preparation was titrated to pH 12.10 with 1.25 M NaOH, incubated for 10 min at room temperature, and neutralized with 6 M HCl to pH 7.8, a procedure known to selectively denature linear and noncovalently closed (form II) circular DNAs (9). This lysate was then extracted with 1/2 vol of redistilled phenol (equilibrated with 0.15 M EDTA), and nucleic acids in the aqueous phase were precipitated overnight at -20'C by the addition of 2 vol of cold 100% ethanol. After centrifugation and et...

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“…Recent studies have demonstrated heterogeneity among the human pap illoma viruses at least with the plantar, common and flat warts [3][4][5][6], Con dylomata acuminata have been shown to contain a papilloma virus which appeared to differ from the other human wart viruses when tested immunologically [7,8] and biochemically [9][10][11][12]. These results were obtained by nucleic acid hybridization tests using viral DNA directly extracted from the tissues.…”

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confidence: 64%

Human Papilloma Virus Type I Purified from Human Genital Warts

Staquet¹,

Viac²,

Bustamante³

et al. 1981

Dermatology

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Human wart virus (HPV) was isolated from a pool of genital warts. The electrophoretic mobility of virion proteins was studied by SDS polyacrylamide gel electrophoresis and showed the same pattern as that obtained with HPV-1. The analysis of DNA after restriction enzyme digestion with the endonucleases Hind III and Hae III, and nucleic acid hybridization did not show any difference with HPV-1. The viral particles were agglutinated by anti-HPV-1 serum, as shown by electron microscopic particle agglutination test. Furthermore, the immunological properties of this virus were investigated with guinea pig antiserum. Serologically, no antigenic cross-reaction between common and genital wart viruses were shown by immunodiffusion and immunofluorescence tests, whereas cross-reactions were detected between plantar and genital wart viruses. These results allow to think that HPV-1 can induce plantar warts as well as genital warts.

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The absence of human papilloma viral DNA sequences in condylomata acuminata

Cited by 26 publications

References 16 publications

Twenty-One Years of Follow-Up Studies of Familial Epidermodysplasia verruciformis

Twenty-One Years of Follow-Up Studies of Familial Epidermodysplasia verruciformis

Change in quantity and size distribution of small circular DNAs during development of chicken bursa.

Human Papilloma Virus Type I Purified from Human Genital Warts

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