Human Papilloma Virus Type I Purified from Human Genital Warts

Abstract

Human wart virus (HPV) was isolated from a pool of genital warts. The electrophoretic mobility of virion proteins was studied by SDS polyacrylamide gel electrophoresis and showed the same pattern as that obtained with HPV-1. The analysis of DNA after restriction enzyme digestion with the endonucleases Hind III and Hae III, and nucleic acid hybridization did not show any difference with HPV-1. The viral particles were agglutinated by anti-HPV-1 serum, as shown by electron microscopic particle agglutination test. Furthermore, the immunological properties of this virus were investigated with guinea pig antiserum. Serologically, no antigenic cross-reaction between common and genital wart viruses were shown by immunodiffusion and immunofluorescence tests, whereas cross-reactions were detected between plantar and genital wart viruses. These results allow to think that HPV-1 can induce plantar warts as well as genital warts.

Synthesis and assembly of infectious bovine papillomavirus particles in vitro

“…We have shown, using vaccinia virus (W) recombinants expressing the L1 and L2 capsid proteins of HPV type 16 (HPV-16), that particles resembling papillomavirus capsids can be produced in vitro (Zhou et al, 1991). CV-1 cells infected with a VV double recombinant for the L1 and L2 open reading frames (ORFs) of HPV-16 produced virus-like particles (VLPs; Zhou et al, 1991), with capsomeres similar in appearance to those seen in HPV-1 and bovine PV type 1 (BPV-1) particles purified from clinical lesions (Baker et al, 1991 ;Staquet et al, 1981). However, the morphology of the HPV-16 VLPs was different to naturally occurring HPV-1 and BPV-1 particles.…”