The ability of the BANA test to detect different levels of P. gingivalis, T. denticola and T. forsythia

Abstract

The aim of this study was to evaluate the ability of the BANA Test to detect different levels of Porphyromonas gingivalis, Treponema denticola and Tannerella forsythia or their combinations in subgingival samples at the initial diagnosis and after periodontal therapy. Periodontal sites with probing depths between 5-7 mm and clinical attachment level between 5-10 mm, from 53 subjects with chronic periodontitis, were sampled in four periods: initial diagnosis (T0), immediately (T1), 45 (T2) and 60 days (T3) after scaling and root planing. BANA Test and Checkerboard DNA-DNA hybridization identified red complex species in the subgingival biofilm. In all experimental periods, the highest frequencies of score 2 (Checkerboard DNA-DNA hybridization) for P. gingivalis, T. denticola and T. forsythia were observed when strong enzymatic activity (BANA) was present (p < 0.01). The best agreement was observed at initial diagnosis. The BANA Test sensitivity was 95.54% (T0), 65.18% (T1), 65.22% (T2) and 50.26% (T3). The specificity values were 12.24% (T0), 57.38% (T1), 46.27% (T2) and 53.48% (T3). The BANA Test is more effective for the detection of red complex pathogens when the bacterial levels are high, i.e. in the initial diagnosis of chronic periodontitis.

Periodontal Disease Activity Measured by the Benzoyl‐DL‐Arginine‐Naphthylamide Test Is Associated With Preterm Births

“…The use of DNA probes is a laboratory‐based procedure that requires equipment and resources that were not available for the present study, whereas the BANA test could be performed at chairside and lended itself to the type of field study described. The BANA test appears to be a reliable surrogate for the use of DNA probes in the detection of P. gingivalis , T. denticola , and T. forsythia in plaque samples 14 . Bayingana 33 showed that the BANA test was more reflective of gingival conditions during pregnancy than were DNA probes.…”

“…The BANA test appears to be a reliable surrogate for the use of DNA probes in the detection of P. gingivalis, T. denticola, and T. forsythia in plaque samples. 14 Bayingana 33 showed that the BANA test was more reflective of gingival conditions during pregnancy than were DNA probes.…”

Factors affecting oral malodor in periodontitis and gingivitis patients

“…Loesche et al 12 reported that detection of the BANA enzyme in a plaque sample most likely reflects the presence of the red complex in patients. Andrade et al 13 demonstrated that the BANA test was most effective for the detection of the red complex when bacterial levels were high, with a sensitivity of 95.5%. Takaishi et al 14 also found a significant correlation between BANA activity and the presence of the red complex using a polymerase chain reaction method.…”

Treponema denticola: patógeno en procesos periodontales y pulpares

“…19 Técnicas de Biología molecular como PCR(reacción en cadena de la polimerasa), no permiten aislar al T. denticola, pero si determinan su presencia y cantidad, pruebas como PCR convencional, PCR Nested y PCR en tiempo real, son muy usados en su investigación.…”