The ability of fetal calf serum, new-born calf serum and normal steer serum to promote the development of bovine morulae

Allen, R.L.; Bondioli, K. R.; Wright, Raymond W.

doi:10.1016/0093-691x(82)90102-9

Cited by 16 publications

(3 citation statements)

References 6 publications

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“…Moreover, the results of the current study come in accordance with the study of Allen et al 31 who found that supplementation of Hams F-10 tissue culture medium with 5% or 10% NBS was effective than FCS ( P < .05) in promoting the in vitro development of bovine morulae. Also, a study of Watson et al 32 reported that the supplementation of tissue culture medium with NBS resulted in greater blastocyst rates than when cultured in SOF medium.…”

Section: Discussionsupporting

confidence: 92%

The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system

Abdel‐Wahab

Aziz

Helmy

et al. 2018

Porto Biomedical Journal

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This study was conducted to assess the effects of addition of 10% newborn bovine serum (NBS) in the maturation and culture media of bovine system on oocytes maturation and developed morula and blastocyst rates. For this purpose, 10% NBS was added to in vitro maturation (IVM) medium alone (Experiment I), in vitro culture (IVC) (Experiment II), and in IVM + IVC (Experiment III). In vitro bovine oocytes maturation and developmental rates of bovine embryos were then compared with control group where the basal maturation or culture media were supplemented with 10% fetal calf serum (FCS). Results revealed that maturation, cleavage, morula, and blastocyst formation rates of in vitro fertilized embryos were not significantly different in NBS and control groups in the 3 experiments. In conclusion, NBS is an efficient macromolecule in bovine system when added either to IVM or to IVC or to both stages. Thus, NBS could provide cheaper and more available treatment to be used as FCS substitute in the IVC system of bovines.

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Section: Discussionsupporting

confidence: 92%

The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system

Abdel‐Wahab

Aziz

Helmy

et al. 2018

Porto Biomedical Journal

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“…This process is obligatory for IVF in contrast to some of the aspects mentioned above. Since the 1980s several studies have focused on investigating the effects of culture media in implantation, survival and development in different animal species, and the influence of several modifications in culture media such as the effects of proteins, the quality of the water and serum [ 93 – 99 ]. An increasing number of studies confirm the influence of the culture medium in epigenetic preimplantation reprogramming and its impact on early embryo development [ 100 – 102 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro fertilization (IVF) in mammals: epigenetic and developmental alterations. Scientific and bioethical implications for IVF in humans

et al. 2015

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The advent of in vitro fertilization (IVF) in animals and humans implies an extraordinary change in the environment where the beginning of a new organism takes place. In mammals fertilization occurs in the maternal oviduct, where there are unique conditions for guaranteeing the encounter of the gametes and the first stages of development of the embryo and thus its future. During this period a major epigenetic reprogramming takes place that is crucial for the normal fate of the embryo. This epigenetic reprogramming is very vulnerable to changes in environmental conditions such as the ones implied in IVF, including in vitro culture, nutrition, light, temperature, oxygen tension, embryo-maternal signaling, and the general absence of protection against foreign elements that could affect the stability of this process. The objective of this review is to update the impact of the various conditions inherent in the use of IVF on the epigenetic profile and outcomes of mammalian embryos, including superovulation, IVF technique, embryo culture and manipulation and absence of embryo-maternal signaling. It also covers the possible transgenerational inheritance of the epigenetic alterations associated with assisted reproductive technologies (ART), including its phenotypic consequences as is in the case of the large offspring syndrome (LOS). Finally, the important scientific and bioethical implications of the results found in animals are discussed in terms of the ART in humans.

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“…In light of the ART strategies, the use of FCS as a growth medium supplement in cell culture has increased widely. In essence, the benefit of using FCS in maturation media has increased since it contains some undefined growth-promoting components such as feutin which is not available in the serum of adult animals (39). Besides, it does not contain any hormones or high levels of immunoglobulins that impede the development of oocytes in vitro (40).…”

Section: Fcsmentioning

confidence: 99%

Role of Some Inducing Additives in in vitro Maturation Environment of Farm Animal's Oocytes: Status Evaluation

Mardenli¹,

salih²,

Hassooni³

et al. 2022

QJAS

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This review focused on highlighting the importance of the biochemical role of some additives that have a stimulating impact on in vitro maturation (IVM) of farm animal oocytes. The supportive role that these additives played has led to a noticeable increase in embryos yields. Nevertheless, there are some considerations that must be taken into account for the process of producing embryos in vitro to function optimally. Among the hormones, the famous gonadotropic hormones (follicle-stimulating hormone (FSH), luteinizing hormone (LH) and chorionic gonadotropin hormone (CG)), estradiol and growth hormone (GH) occupied a wide area in IVM, but the usage of these hormones still limited to specific concentrations as the increase in their concentrations did not give the desired effect. Due to the historical popularity and the primary role of cysteamine as an antioxidant agent, its usage has increased recently. However, this usage has also remained limited to specific concentrations, as are hormones. Besides, cysteamine was an important reason for obtaining high rates of embryos in the blastocyst stage. Conversely, there was a reservation in the use of follicular fluid (FF) and fetal calf serum (FCS). Although these two elements are rich in hormones, proteins, lipids and various growth factors, FF contains factors that inhibit the maturation of oocytes. Moreover, FCS has a negative role in the quality of resulted embryos because of the high levels of lipids (triglycerides).

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The ability of fetal calf serum, new-born calf serum and normal steer serum to promote the development of bovine morulae

Cited by 16 publications

References 6 publications

The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system

The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system

In vitro fertilization (IVF) in mammals: epigenetic and developmental alterations. Scientific and bioethical implications for IVF in humans

Role of Some Inducing Additives in in vitro Maturation Environment of Farm Animal's Oocytes: Status Evaluation

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