The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system

Abdel‐Wahab, Ahmed; Aziz, R.L. Abdel; Helmy, Nadia; Ibrahim, Shawky S.

doi:10.1016/j.pbj.0000000000000003

Cited by 4 publications

(2 citation statements)

References 26 publications

(30 reference statements)

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“…They can also influence oocyte maturation in culture by stimulating granulosa cells and enhancing the rate of oocytes in the second metaphase (M II) (Richani and Gilchrist, 2018). FBS, as a supplement, also provides growth factors and other important molecules for oocyte maturation and embryonic development, including vitamins, hormones, signaling proteins, and carrier proteins that fulfill the physiological needs of cultured cells in the presence of the blood supplement (Abdel-Wahab et al, 2018). Modifying FBS with PRP does not seem to have a significant effect on increasing or decreasing embryo production rates, as both supplements have complex compositions and provide important molecules for COCs.…”

Section: Discussionmentioning

confidence: 99%

Use of platelet-rich plasma on in vitro maturation during bovine embryo production

Souza,

Marin,

Ramos

et al. 2024

Anim. Reprod.

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One of the crucial aspects to be considered for successful in vitro production (IVP) of embryos is the composition of the various media used throughout the stages of this reproductive biotechnology. The cell culture media employed should fulfill the metabolic requirements of both gametes during oocyte maturation and sperm development, as well as the embryo during its initial cell divisions. Most IVP protocols incorporate blood serum into the media composition as a source of hormones, proteins, growth factors, and nutrients. Numerous studies have suggested Platelet-Rich Plasma (PRP) as a substitute for fetal sera in cell culture, particularly for stem cells. Therefore, the objective of this study is to assess the potential use of PRP as a replacement for fetal bovine serum (FBS) during oocyte maturation for in vitro production of bovine embryos. During in vitro maturation (IVM), cumulus-oocyte complexes (COCs) were allocated into the following experimental groups: Group G1 (IVM medium with 5% PRP); Group G2 (MIV medium with 5% PRP and 5% SFB); Group G3 (MIV medium with 5% SFB); and Group G4 (MIV medium without either PRP or SFB). Subsequently, the cumulus-oocyte complexes were fertilized with semen from a single bull, and the resulting zygotes were cultured for seven days. Cleavage and blastocyst formation rates were assessed on days 2 and 7 of embryonic development, respectively. The quality of matured COCs was also evaluated by analyzing the gene expression of HSP70, an important protein associated with cellular stress. The results demonstrated that there were no significant differences among the experimental groups in terms of embryo production rates, both in the initial cleavage stages and blastocyst formation (except for the G4 group, which exhibited a lower blastocyst formation rate on D7, as expected). This indicates that PRP could be a cost-effective alternative to SFB in the IVP of embryos.

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Section: Discussionmentioning

confidence: 99%

Use of platelet-rich plasma on in vitro maturation during bovine embryo production

Souza,

Marin,

Ramos

et al. 2024

Anim. Reprod.

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“…Porcine zygotes of various origins, either in vitro fertilization (IVF), parthenogenesis, or somatic cell nuclear transfer, have been reported to have higher development rates in PZM-3 media than in NCSU23 media [ 8 ]. It was also reported that the embryonic development rate was increased when the medium was supplemented with fetal bovine serum (FBS) [ 9 ] or fetal calf serum (FCS) [ 10 ]. It was proved that supplementation of animal serum acts as an energy source, osmoregulator, pH stabilizers, numerous antioxidants, and growth factors [ 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo

Biswas¹,

Hyun²

2021

J Adv Vet Anim Res

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Objective: The present study aimed to explain the effect of fetal bovine serum (FBS) on the in vitro production of porcine embryos and the molecular effects of FBS on the growing of porcine embryos. Materials and Methods: Immature porcine oocytes were matured and fertilized in vitro . The resulting zygotes were cultured in porcine zygotic medium-3- until day 7 and FBS was added on day 4. Without FBS, it was treated as a control group. Quantitative real-time PCR and 2′,7′-dichloro-dihydro-fluorescein diacetate (H 2 DCFDA) molecular staining techniques were used to detect the expression patterns of apoptosis-associated genes and the accumulation of reactive oxygen species (ROS), respectively. Paired student’s t -test was used by GraphPad Prism statistical software. Results: FBS supplementation boosted blastocyst (BL) development and total cell count per BL substantially ( p < 0.05). However, hatching and hatched BLs also increased in the FBS-treated group compared to the control. We also found that ROS accumulation in FBS-treated embryos was significantly reduced ( p < 0.05) compared to the control group. The expression of the anti-apoptotic gene BCL-2 was significantly increased in FBS-treated BLs, but the pro-apoptotic gene, caspase-3 expression, was significantly reduced in FBS-treated BLs. Conclusion: Our results suggest that FBS supplementation in porcine culture media could increase porcine embryo production by decreasing ROS accumulation and increasing the anti-apoptotic gene expression in developing BLs.

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In Vitro Fertilization

Hasler¹,

Barfield

2021

Bovine Reproduction

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The impact of using newborn bovine serum as fetal calf serum substitute in the in vitro bovine embryos production system

Cited by 4 publications

References 26 publications

Use of platelet-rich plasma on in vitro maturation during bovine embryo production

Use of platelet-rich plasma on in vitro maturation during bovine embryo production

Supplementation of fetal bovine serum increased the quality of in vitro fertilized porcine embryo

In Vitro Fertilization

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