The aberrantly expressed long non‐coding <scp>RNA</scp> in the substantia nigra and corpus striatum of Nrf2‐knockout mice

Liu, Jian; Xu, Yali; Kang, Yunxiao; Cao, Shanhu; Shi, Geming; Cui, Huixian; Sun, Shao-Guang; Wang, Lei

doi:10.1111/jnc.14141

Cited by 11 publications

(4 citation statements)

References 36 publications

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“…Our previous study has identified 96 and 643 DEmRNAs in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice, respectively [28]. After these DEmRNAs were retained to construct the DEcircRNA-miRNA-DEmRNA interaction network, mmu_circRNA_015216, mmu_circRNA_32463, mmu_circRNA_34106 and mmu_circRNA_34132 were predicted to compete with 10, 11, 8 and 17 DEmRNAs in the substantia nigra between Nrf2 (-/-) and Nrf2 (+/+) mice, respectively; and mmu_circRNA_33836, mmu_circRNA_017077 and mmu_circRNA_003119, mmu_circRNA_34132 and mmu_circRNA_34106 were predicted to compete with 22, 80 and 18, 169 and 78 DEmRNAs, respectively, in the corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice.…”

Section: Decircrna-mirna-demrna Interaction Networkmentioning

confidence: 99%

The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice

Yang¹,

Zhang²,

Lin³

et al. 2018

Cell Physiol Biochem

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Background/Aims: The nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway plays a protective role in both acute neuronal damage and chronic neurodegeneration-related oxidative stress. Circular RNAs (circRNAs) are involved with various diseases in the central nervous system (CNS). This study aimed to identify the key circRNAs involved in Nrf2-neuroprotection against oxidative stress. Methods: The differentially expressed circRNAs (DEcircRNAs) in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice were identified by microarray analysis. Quantitative real-time polymerase chain reaction (qRT-PCR) was then used to validate the expression of selected DEcircRNAs in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice. Based on our previous microarray analysis of the differentially expressed mRNAs (DEmRNAs) in the substantia nigra and corpus striatum between Nrf2 (-/-) and Nrf2 (+/+) mice, the DEcircRNA-miRNA-DEmRNA interaction network was constructed. Functional annotation of DEmRNAs that shared the same binding miRNAs with DEcircRNAs was performed using gene ontology (GO) and pathway analyses. Results: A total of 65 and 150 significant DEcircRNAs were obtained in the substantia nigra and corpus striatum of Nrf2 (-/-) mice, respectively, and seventeen shared DEcircRNAs were found in both these two tissues. The qRT-PCR results were generally consistent with the microarray results. The DEcircRNA-miRNA-DEmRNA interaction network and pathway analysis indicated that mmu_circRNA_34132, mmu_circRNA_017077 and mmu-circRNA-015216 might be involved with Nrf2-mediated neuroprotection against oxidative stress. Mmu_circRNA_015216 and mmu_circRNA_017077 might play roles in the Nrf2-related transcriptional misregulation and Nrf2-mediated processes of rheumatoid arthritis, respectively. In addition to these two processes, mmu_circRNA_34132 may be a potential regulator of Nrf2-mediated protection for diabetes mellitus and Nrf2-mediated defence against ROS in hearts. Conclusion: In conclusion, our study identified the key DEcircRNAs in the substantia nigra and corpus striatum of Nrf2 (-/-) mice, which might provide new clues for further exploring the mechanism of Nrf2-mediated neuroprotection against oxidative stress and other Nrf2-mediated processes.

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Section: Decircrna-mirna-demrna Interaction Networkmentioning

confidence: 99%

The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice

Yang¹,

Zhang²,

Lin³

et al. 2018

Cell Physiol Biochem

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“…Further, 279/164 upregulated lncRNAs and 477/177 downregulated lncRNAs were found in -SNCA * A30P * A53T transgenic mice and alpha-synuclein transgenic mice [53,55], respectively. In Nrf2 knockout mice, 74 upregulated lncRNAs and 160 downregulated lncRNAs were found [54]. Differential lncRNA expression in conventional nontransgenic PD models has also been studied.…”

Section: Aberrant Expression Of Lncrnas In Pdmentioning

confidence: 99%

Role of Long Noncoding RNAs in Parkinson’s Disease: Putative Biomarkers and Therapeutic Targets

Wang

Zhong

et al. 2020

Parkinson's Disease

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Parkinson’s disease (PD) is a neurodegenerative disease characterized by bradykinesia, rigidity, and tremor. Age is the main risk factor. Long noncoding RNAs (lncRNAs) are novel RNA molecules of more than 200 nucleotides in length. They may be involved in the regulation of many pathological processes of PD. PD has a variety of pathophysiological mechanisms, including alpha-synuclein aggregate, mitochondrial dysfunction, oxidative stress, calcium homeostasis, axonal transport, and neuroinflammation. Among these, the impacts of lncRNAs on the pathogenesis and progression of PD need to be highlighted. lncRNAs may serve as putative biomarkers and therapeutic targets for the early diagnosis of PD. This study aimed to investigate the role of lncRNAs in various pathological processes of PD and the specific lncRNAs that might be used as putative diagnostic biomarkers and therapeutic targets of PD.

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“…In our previous study, we detected the altered expression of circRNAs and lncRNAs in the substantia nigra and corpus striatum tissue of Nrf2-null mice (Liu J. et al, 2017; Yang et al, 2018), and considering that Nrf2 activity may diverge across different brain regions, the current study focused on the expression profiles of circRNAs and lncRNAs in the hippocampus tissue of Nrf2-null mice. Previous studies showed definitively that the hippocampus and neighboring structures of the medial temporal lobe are essential to memory (Preston and Eichenbaum, 2013).…”

Section: Introductionmentioning

confidence: 99%

Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

Zhang

Liu

et al. 2019

Front. Mol. Neurosci.

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Background Nrf2 (nuclear factor, erythroid 2 like 2) is believed to play a major role in neurodegenerative diseases. The present study attempts to investigate the hippocampal circRNA and lncRNA expression profiles associated with Nrf2-mediated neuroprotection. Methods The hippocampal mRNA, circRNA and lncRNA expression profiles of Nrf2 (−/−) mice were determined by a microarray analysis. Bioinformatics analyses, including identification of differentially expressed mRNAs (DEmRNAs), circRNAs (DEcircRNAs) and lncRNAs (DElncRNAs), DEcircRNA-miRNA-DEmRNA interaction network construction, DElncRNA-DEmRNA co-expression network construction, and biological function annotation, were conducted. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to validate the dysregulated expression of circRNAs and lncRNAs derived from the microarray data of the hippocampus of Nrf2 (−/−) mice. Results Compared to wild-type Nrf2 (+/+) mice, 412 DEmRNAs (109 up- and 303 down-regulated mRNAs), 1279 DEcircRNAs (632 up- and 647 down-regulated circRNAs), and 303 DElncRNAs (50 up- and 253 down-regulated lncRNAs) were identified in the hippocampus of Nrf2 (−/−) mice. Additionally, in the qRT-PCR validation results, the expression patterns of selected DEcircRNAs and DElncRNAs were generally consistent with results in the microarray data. The DEcircRNA-miRNA-DEmRNA interaction networks revealed that mmu_circRNA_44531, mmu_circRNA_34132, mmu_circRNA_000903, mmu_circRNA_018676, mmu_circRNA_45901, mmu_circRNA_33836, mmu_circRNA_ 34137, mmu_circRNA_34106, mmu_circRNA_008691, and mmu_circRNA_003237 were predicted to compete with 47, 54, 45, 57, 63, 81, 121, 85, 181, and 43 DEmRNAs, respectively. ENSMUST00000125413, NR_028123, uc008nfy.1, AK076764, AK142725, AK080547, and AK035903 were co-expressed with 178, 89, 149, 179, 142, 55, and 112 DEmRNAs in the Nrf2 (−/−) hippocampus, respectively. Conclusion Our study might contribute to exploring the key circRNAs and lncRNAs associated with Nrf2-mediated neuroprotection.

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The aberrantly expressed long non‐coding RNA in the substantia nigra and corpus striatum of Nrf2‐knockout mice

Cited by 11 publications

References 36 publications

The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice

The Differentially Expressed Circular RNAs in the Substantia Nigra and Corpus Striatum of Nrf2-Knockout Mice

Role of Long Noncoding RNAs in Parkinson’s Disease: Putative Biomarkers and Therapeutic Targets

Differential Expression Profiles and Functional Prediction of Circular RNAs and Long Non-coding RNAs in the Hippocampus of Nrf2-Knockout Mice

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