The AAA-ATPase MIDASIN 1 Functions in Ribosome Biogenesis and Is Essential for Embryo and Root Development

Li, Pengcheng; Li, Ké; Wang, Juan; Zhao, Chuanzhi; Zhao, Shuzhen; Hou, Lei; Xia, Han; Ma, Changle; Wang, Xingjun

doi:10.1104/pp.18.01225

Cited by 20 publications

(32 citation statements)

References 64 publications

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“…Previously, we reported that the germination rate of the mdn1-1 seeds was significantly lower than that of wild type under normal growth conditions (Li et al, 2016). We also found that many of the mdn1-1 seeds were shriveled and not viable due to impaired embryogenesis (Li et al, 2019). However, the plump seeds of mdn1-1 appeared to be slightly larger than that of wild type ( Figure 1A ).…”

Section: Resultsmentioning

confidence: 88%

“…mdn1-2 is a knockout allele of MDN1 based on a T-DNA insertion, and its homozygote is embryonic lethal (Li et al, 2016; Li et al, 2019). In previous work, we obtained hybrids by crossing heterozygous mdn1-2 with homozygous mdn1-1 , and the viable mdn1-1 mdn1-2 (bi-allelic mutations in MDN1 ) mutant was further isolated from the hybrids (Li et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…The wild type used in this study is Arabidopsis thaliana ecotype Col-0. The mdn1-1 , mdn1-2 (Salk_057010), and mdn1-1 mdn1-2 (also called dsr1 mdn1 , bi-allelic mutations in MDN1 ) mutants were described in our previous studies (Li et al, 2016; Li et al, 2019). The relative expression levels of MDN1 in mdn1-1 , heterozygous mdn1-2 , and mdn1-1 mdn1-2 are reported in Supplementary Figure 1 .…”

Section: Methodsmentioning

confidence: 93%

“…Total RNA was isolated using a RNAiso Plus Reagent Kit (Takara, Japan) and further purified with a DNase Kit (Takara, Japan). RT-qPCR was performed according to our previous study (Li et al, 2019). The primers for detecting SESA3 mRNA were 5’-AAGACAGAGATGCCAGAA-3’ and 5’-CCTCATTTGCTTGCTCAT-3.’ The primer set for detecting SESA4 mRNA was 5’-ACCAGAGCAAGTCAGGAA-3’ and 5’-TTAGTAGTAAGAAGGGATTGAAGG-3.’ The primer set for detecting EM1 mRNA was 5’-GGACTCAGTACGATGGAA-3’ and 5’-TTGTTGGTGAACTTTGACT-3.’ The primers for detecting the MDN1 mRNA were reported in our previous study (Li et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…mdn1-1 (also called dwarf and short root 1 ) is a weak mutant of the Arabidopsis midasin 1 ( MDN1 ) gene resulting from a site-changed mutation (Li et al, 2016). Our previous studies reported that MDN1 is involved in nuclear export of the pre-60S ribosomal particle (Li et al, 2019), and its mutation leads to pleiotropic development phenotypes (Li et al, 2016). In this study, we further found that mdn1-1 has larger seeds than wild type, and the low germination rate of its seeds is mediated by ABI5 to some extent.…”

Section: Introductionmentioning

confidence: 99%

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Arabidopsis MDN1 Is Involved in the Establishment of a Normal Seed Proteome and Seed Germination

Zhou

et al. 2019

Front. Plant Sci.

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Seed germination and formation are the beginning and ending, respectively, of a plant life cycle. These two processes are under fine regulation by the internal genetic information. Previously, we demonstrated that Arabidopsis MIDASIN 1 (MDN1) is required for ribosome biogenesis, and its dysfunction leads to pleiotropic developmental phenotypes, including impaired embryogenesis and slow seed germination. In this study, we further found that the weak mutant of MDN1, mdn1-1, exhibits an increased seed size phenotype. Seed proteomic analysis reveals that a number of proteins involved in seed development and response to external environments are mis-regulated by the MDN1 dysfunction. Many 2S seed storage proteins (SSPs) and late embryogenesis abundant (LEA) proteins are over-accumulated in the dry seeds of mdn1-1. Further, some genes encoding seed storage reserves are also upregulated in mdn1-1 seedlings. More interestingly, abscisic acid-insensitive 5 (ABI5) is over-accumulated in mdn1-1 seeds, and the loss of its function partially rescues the low seed germination rate of mdn1-1. Together, this study further demonstrates that MDN1 is essential for establishing a normal seed proteome, and its mutation triggers ABI5-mediated repression of seed germination.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 93%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Arabidopsis MDN1 Is Involved in the Establishment of a Normal Seed Proteome and Seed Germination

Zhou

et al. 2019

Front. Plant Sci.

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Arabidopsis NOTCHLESS plays an important role in root and embryo development

Zhang

Liu

et al. 2023

Plant Signaling & Behavior

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Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis ( A. thaliana ) remains exclusive. Here, we found that Arabidopsis NLE ( AtNLE ) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.

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Coordination between MIDASIN 1-mediated ribosome biogenesis and auxin modulates plant development

Zhou

Sun

et al. 2021

Journal of Experimental Botany

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Ribosomes are required for plant growth and development, and ribosome-biogenesis-deficiency mutants generally display auxin-related phenotypes. Although the relationship between ribosome dysfunction and auxin has been raised, many aspects of this subject remain to be understood. We previously reported that MIDASIN 1 (MDN1) is an essential pre-60S ribosome biogenesis factor (RBF) in Arabidopsis. In this study, we further characterized the aberrant auxin-related phenotypes of mdn1-1, a weak mutant allele of MDN1. Auxin response is disturbed in both shoots and roots of mdn1-1 indicated by the DR5:GUS reporter. Through combining transcriptomic profiling analysis and reporter detection, we found that expression of genes involved in auxin biosynthesis, transport, and signaling is changed. Furthermore, the MDN1 deficiency affects both the posttranscriptional regulation and protein distribution of PIN2 (an auxin efflux facilitator) in mdn1-1 roots. These results indicate that MDN1 is required for maintaining the auxin system. More interestingly, MDN1 is an auxin-responsive gene, and its promoter can be targeted by multiple AUXIN RESPONSE FACTORs (ARFs), including ARF7 and ARF19, in vitro. Indeed, in arf7 arf19, the auxin sensitivity of MDN1 expression is significantly reduced. Together, our results reveal a coordination mechanism between the auxin system with the MDN1-dependent ribosome biogenesis for regulating plant development.

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The AAA-ATPase MIDASIN 1 Functions in Ribosome Biogenesis and Is Essential for Embryo and Root Development

Cited by 20 publications

References 64 publications

Arabidopsis MDN1 Is Involved in the Establishment of a Normal Seed Proteome and Seed Germination

Arabidopsis MDN1 Is Involved in the Establishment of a Normal Seed Proteome and Seed Germination

Arabidopsis NOTCHLESS plays an important role in root and embryo development

Coordination between MIDASIN 1-mediated ribosome biogenesis and auxin modulates plant development

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