Arabidopsis NOTCHLESS plays an important role in root and embryo development

Abstract

Ribosome biogenesis is a fundamental process in eukaryotic cells. NOTCHLESS (NLE) is involved in 60S ribosome biogenesis in yeast, but its role in Arabidopsis ( A. thaliana ) remains exclusive. Here, we found that Arabidopsis NLE ( AtNLE ) is highly conservative in phylogeny, which encoding a WD40-repeat protein. AtNLE is expressed in actively dividing tissues. AtNLE-GFP is localized in the nucleus. AtNLE physically interacts with the MIDAS domain of AtMDN1, a protein involved in the biogenesis of the 60S ribosomal subunit in Arabidopsis. The underexpressing mutant nle-2 shows short roots and reduced cell number in the root meristem. In addition, the null mutant nle-1 is embryo lethal, and defective embryos are arrested at the early globular stage. This work suggests that AtNLE interacts with AtMDN1, and AtNLE functions in root and embryo development.

Three pollen QTLs are sufficient to partially break stylar unilateral incompatibility ofSolanum pennellii

“…WD40 domains typically assemble into a βpropellers to provide binding sites for other proteins and foster transient interactions for these proteins (Jain & Pandey, 2018). In plants, WDR proteins are reported to be involved in a variety of cellular and developmental process, e.g., cell fate determination, cell division and cytokinesis, light signaling, flowering, and meristem organization (Airoldi et al, 2019, Schenk et al, 2021, Li et al, 2023a. Although the two homologous WDR proteins are supposed to play a role in UI, further validation (e.g., functional analysis of LA0716 pollen with double-knockout pui WDR genes) is required to elucidate their function.…”