The A0 blood group genotype modifies the jejunal glycomic binding pattern profile of piglets early associated with a simple or complex microbiota1

Abstract

The intestinal epithelium glycocalyx sugar motif is an important determinant of the bacterial-host interaction and may be affected in pigs by gut microbiota and by blood group genotype. The aim was to study the effect of intestinal association with different microbiota and A0 blood group genotypes on the expressed glycomic pattern in the small intestine. Twelve caesarean-derived pigs previously associated with a simple association (SA) or complex association (CA) microbiota were selected at 26 to 37 d of age. In each subject, different jejunal loops were perfused for 8 h with enterotoxigenic K88 (ETEC), ETEC fimbriae (F4), (LAM), or a saline control. The piglets were genotyped for A0 blood group and the glycomic profile was evaluated by microscopic screening of lectin binding: peanut agglutinin (PNA), which is galactose specific; agglutinin I (UEA), which is fucose specific; lectin II (MALii), which is sialic acid specific; concavalin A, which is mannose specific; soybean agglutinin (SBA), which is -acetyl-galactosamine specific; and wheat germ agglutinin (WGA), which is -acetyl-glucosamine specific. A0 pigs had fewer UEA-positive cells, MALii-positive cells ( < 0.001), and SBA-positive cells ( < 0.10) than 00 pigs. Simple association pigs had more SBA positive cells ( < 0.01) than CA pigs. Enterotoxigenic K88-perfused intestinal loops had fewer UEA-positive cells ( < 0.01) and WGA positive cells ( < 0.001) cells and more PNA positive cells (only in SA pigs, < 0.01). No effects of introduction of F4 and LAM in the intestinal lumen were observed. The porcine A0 blood group genotype and the luminal presence of ETEC strongly affected the jejunal mucosa glycomic pattern profile whereas an early oral simple or complex microbial association had limited effects. Pig genetic background has relevance on the cross talk between intestinal epithelium glycocalyx sugar motif and ETEC and, ultimately, on the gut microbial colonization in later life.

Effect of feed supplementation with live yeast on the intestinal transcriptome profile of weaning pigs orally challenged with Escherichia coli F4

“…ETEC endotoxins affect also the morphology of the intestinal mucosa (Priori et al, 2016). In 28-day-old healthy pigs, the administration of S. boulardii improved the ileal morphology and increased the cell mitosis rate (Bontempo et al, 2006).…”

Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs

“…Transverse (5 µm thick) sections were obtained from jejunum and analysed for Ulex europaeus agglutinin I (UEA; Vector Laboratories, Peterborough, UK; catalogue n. B‐1065‐ fucose‐specific) and peanut agglutinin (PNA; Vector Laboratories, Peterborough, UK; catalogue n. B‐1075‐ galactose‐specific) following the protocol described by Priori et al (). For each slide, 20 villi and 20 crypts were randomly observed.…”

Molecular networks affected by neonatal microbial colonization in porcine jejunum, luminally perfused with enterotoxigenic Escherichia coli, F4ac fimbria or Lactobacillus amylovorus

“…Villus height and crypt depth are considered markers of the health status of the mucosa. In our previous report, based on the same study [ 31 ], no effect of the early association type was seen on villus height and crypt depth nor a correlation of association treatment with loop perfusion treatments. Conversely, we showed a significant decrease of villous height and villous surface in ETEC challenged loops compared to CTRL, indicating a worsening of functional status of the jejunal mucosa.…”

“…Thus, the triggering of the SLC26A3 gene by LAM may compensate at least in part the endotoxin-induced activation of chloride channels and may reduce the occurrence of diarrhea. This can explain the small improvement of villus height in LAM-perfused loops [ 31 ]. Also Lactobacillus acidophilus stimulated the expression of SLC26A3 via a transcriptional mechanism [ 47 ].…”