The 9cis,11trans,13cisIsomer of Conjugated Linolenic Acid Reduces Apolipoprotein B100 Secretion and Triacylglycerol Synthesis in HepG2 Cells

Arao, Keisuke; Yotsumoto, Hiroaki; Han, Sang Hoon; Nagao, Koji; Yanagita, Teruyoshi

doi:10.1271/bbb.68.2643

Cited by 55 publications

(53 citation statements)

References 21 publications

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“…Current data on the effect of PSO on lipid concentration and metabolism are controversial (8,9,17) . Similar to our findings, the liver accumulation of TAG was reduced by PSO administration (8,18) ; a plausible mechanism, demonstrated in an in vitro study, that 9-cis, 11-trans, 13-cis CLN suppressed cellular synthesis of TAG in HepG2 cells (19) .…”

Section: Discussionsupporting

confidence: 90%

Effect of pomegranate seed oil on hyperlipidaemic subjects: a double-blind placebo-controlled clinical trial

et al. 2010

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In vitro and in vivo studies have shown that punicic acid, a type of conjugated fatty acid and the main constituent of pomegranate seed oil (PSO), has anti-atherogenic effects. The present study aimed at determining the effect of PSO treatment on serum lipid profiles. This double-blind placebo-controlled randomised clinical trial included fifty-one hyperlipidaemic subjects, diagnosed according to National Cholesterol Education Program definition, and randomly assigned to the PSO and the control groups. The PSO and placebo groups received 400 mg PSO and placebo twice daily, respectively and were followed up for 4 weeks. Serum concentrations of lipids and lipoproteins were measured before and 4 weeks after intervention. Mean concentration of TAG and the TAG:HDL cholesterol (HDL-C) ratio were significantly decreased after 4 weeks in the PSO group as compared with baseline values (2·75 (SD 1·40) v. 3·45 (SD 1·56) mmol/l, P¼ 0·009 and 5·7 (SD 4·6) v. 7·5 (SD 5·0), P¼ 0·031, respectively). The treatment effect was statistically significant in the PSO group as compared with controls in diminution of cholesterol:HDL-C ratio (5·4 (SD 1·5) v. 5·9 (SD 1·4), P, 0·05) adjusted for baseline values. We found a mean difference for PSO v. placebo in HDL-C concentration (0·13 v. 20·02 mmol/l) and cholesterol:HDL-C ratio (20·42 v. 0·01, P,0·05). Serum cholesterol, LDL cholesterol and glucose concentrations and body composition variables remained unchanged. It is concluded that administration of PSO for 4 weeks in hyperlipidaemic subjects had favourable effects on lipid profiles including TAG and TAG:HDL-C ratio. Pomegranate seed oil: Hyperlipidaemia: Randomised clinical trialsNormalisation of dyslipidaemia, a modifiable risk factor, can prevent or reduce the risk of CVD, which is a leading cause of death worldwide (1,2) .In order to treat dyslipidaemia, different pharmacologic and non-pharmacologic approaches have been used. Statins are the first-line therapy for management of increased LDL cholesterol (LDL-C), despite adverse effects such as gastrointestinal symptoms and muscle aches (3) . Although fibrates may decrease serum TAG and increase serum HDL cholesterol (HDL-C), more powerful drugs for normalisation of these lipids need to be investigated.Conjugated fatty acids are well-known hypolipidaemic agents (4,5) documented for their effect on lipid metabolism (6) . PSO (Punica granatum) consists of about 80 % conjugated octadecatrienoic fatty acids, with a high content of 9-cis, 11-trans, 13-cis acid or punicic acid (PA), one of the isomers of conjugated linolenic acid (CLN) (7) ; animal studies report controversial results for the hypolipidaemic role of PSO (8,9) .Although Arao et al. (8) showed that PSO supplementation in obese hyperlipidaemic Otsuka-Long-Evans-Tokushima fatty rats reduces TAG accumulation, Yang et al. (9) demonstrated that PSO does not alter serum cholesterol concentration.Considering the lack of data on the effect of PSO on lipid profiles in human subjects, the present study investigated the effect of PSO ...

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Section: Discussionsupporting

confidence: 90%

Effect of pomegranate seed oil on hyperlipidaemic subjects: a double-blind placebo-controlled clinical trial

et al. 2010

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“…LA treatments tended to increase [1-14 C] oleate incorporation into cellular TG, which meant enhanced TG synthesis, but the increase was significantly reduced by LAME as compared with control medium. This result is consistent with our previous report showing that conjugated fatty acids reduced apo B100 secretion through the suppression of TG synthesis in HepG2 cells 26,27) . Hence we consider that the reduction of apo B100 secretion by LAME is at least in part attributable to the suppression of cellular TG synthesis in HepG2 cells.…”

Section: Discussionsupporting

confidence: 94%

Linoleic Acid-Menthyl Ester Reduces the Secretion of Apolipoprotein B100 in HepG2 Cells

et al. 2009

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The effect of linoleic acid-menthyl ester (LAME) on lipid metabolism were assessed in HepG2 cells. It is well known that high level of apolipoprotein (apo) B100 in the serum is risk for atherosclerosis. Although linoleic acid (LA) treatment and LA plus L-mentol treatment increased apo B100 secretion, LAME treatment significantly decreased apo B100 secretion in HepG2 cells compared with control medium. The hypolipidemic effect of LAME was attributable to the suppression of triglyceride synthesis in HepG2 cells. It is also known that the risk of coronary heart disease is negatively related to the concentration of serum apo A-1. In the present study, LAME treatment increased apo A-1 secretion as compared with LA treatment in HepG2 cells. These results suggest that mentyl-esterification of fatty acids may be beneficial in anti-atherogenic dietary therapy.

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“…The three double bonds present in CLNAs are primarily in positions Δ9,11,13 and Δ8,10,12 and exist in both cis and trans geometrical isomer forms (SASSANO et al, 2009). CLNAs have been reported to occur in terrestrial plant lipids, especially seed oils (KINAMI et al, 2007), present in high amounts accounting for 40-80% of total fatty acids (YANG et al, 2005;ARAO et al, 2004b). Five CLNA isomers occur as components of major seed oils of several plants: α-eleostearic acid cis9,trans11,, punicic acid (PA,cis9,trans11,calendic acid (trans8,trans10,jacaric acid (cis8,trans10,, and catalpic acid (trans9, trans11, cis13-18:3) (IWABUCHI; KOHNO-MURASE; IMAMURA, 2003; further studies are needed to provide a clearer understanding of this mechanism of CLNAs metabolism (YUAN, G. F.; YUAN, J. Q.; LI, 2009).…”

Section: Structure Isomers and Sourcesmentioning

confidence: 99%

Chemical and physiological aspects of isomers of conjugated fatty acids

Carvalho¹,

Melo²,

Mancini‐Filho³

2010

Ciênc. Tecnol. Aliment.

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The 9cis,11trans,13cisIsomer of Conjugated Linolenic Acid Reduces Apolipoprotein B100 Secretion and Triacylglycerol Synthesis in HepG2 Cells

Cited by 55 publications

References 21 publications

Effect of pomegranate seed oil on hyperlipidaemic subjects: a double-blind placebo-controlled clinical trial

Effect of pomegranate seed oil on hyperlipidaemic subjects: a double-blind placebo-controlled clinical trial

Linoleic Acid-Menthyl Ester Reduces the Secretion of Apolipoprotein B100 in HepG2 Cells

Chemical and physiological aspects of isomers of conjugated fatty acids

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