The 8q24 region hosts miRNAs altered in biospecimens of colorectal and bladder cancer patients

Gagliardi, Amedeo; Francescato, Giulia; Ferrero, Giulio; Birolo, Giovanni; Tarallo, Sonia; Francavilla, A.; Piaggeschi, Giulia; Battista, Carla Di; Gallo, Gaetano; Luc, A. Realis; Sacerdote, Carlotta; Matullo, Giuseppe; Víneis, Paolo; Naccarati, Alessio; Pardini, Barbara

doi:10.1002/cam4.5375

Cited by 4 publications

(4 citation statements)

References 50 publications

(115 reference statements)

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“…Pearson correlation coefficients (r p ) are shown on title of each plot separately. (F) Correlations of signature genes with risk score for the dataset from Gagliardi et al ( n = 76) [26]. Pearson correlation coefficients (r p ) are shown on title of each plot separately.…”

Section: Resultsmentioning

confidence: 99%

“…Overall significance was determined by One‐Way ANOVA ( p < 0.001) followed by Tukey's post hoc test (significance bars). (H) Risk score values and the corresponding CMS extracted from the Gagliardi et al dataset ( n = 76) [26]. Overall significance was determined by one‐way ANOVA ( p < 0.001) followed by Tukey's post hoc test (significance bars).…”

Section: Resultsmentioning

confidence: 99%

“…Details for the generation of the three-gene signature on GSE39582 and its validation on three additional datasets (GSE17536 [24], an RNA-Seq dataset [25,26], and the TCGA COAD data set [27,28]), as well as gene signature association with clinical data and the EMTome database are explained in detail in Supplementary materials and methods. Differential gene expression was investigated between high-and low-risk group samples of the GSE39582 dataset using the limma package [29], followed by Gene Set Enrichment Analysis (GSEA).…”

Section: In Silico Analysesmentioning

confidence: 99%

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A partial epithelial‐mesenchymal transition signature for highly aggressive colorectal cancer cells that survive under nutrient restriction

Pastorino,

Sheraj,

Huebner

et al. 2024

The Journal of Pathology

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Partial epithelial‐mesenchymal transition (p‐EMT) has recently been identified as a hybrid state consisting of cells with both epithelial and mesenchymal characteristics and is associated with the migration, metastasis, and chemoresistance of cancer cells. Here, we describe the induction of p‐EMT in starved colorectal cancer (CRC) cells and identify a p‐EMT gene signature that can predict prognosis. Functional characterisation of starvation‐induced p‐EMT in HCT116, DLD1, and HT29 cells showed changes in proliferation, morphology, and drug sensitivity, supported by in vivo studies using the chorioallantoic membrane model. An EMT‐specific quantitative polymerase chain reaction (qPCR) array was used to screen for deregulated genes, leading to the establishment of an in silico gene signature that was correlated with poor disease‐free survival in CRC patients along with the CRC consensus molecular subtype CMS4. Among the significantly deregulated p‐EMT genes, a triple‐gene signature consisting of SERPINE1, SOX10, and epidermal growth factor receptor (EGFR) was identified. Starvation‐induced p‐EMT was characterised by increased migratory potential and chemoresistance, as well as E‐cadherin processing and internalisation. Both gene signature and E‐cadherin alterations could be reversed by the proteasomal inhibitor MG132. Spatially resolving EGFR expression with high‐resolution immunofluorescence imaging identified a proliferation stop in starved CRC cells caused by EGFR internalisation. In conclusion, we have gained insight into a previously undiscovered EMT mechanism that may become relevant when tumour cells are under nutrient stress, as seen in early stages of metastasis. Targeting this process of tumour cell dissemination might help to prevent EMT and overcome drug resistance. © 2024 The Authors. The Journal of Pathology published by John Wiley & Sons Ltd on behalf of The Pathological Society of Great Britain and Ireland.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: In Silico Analysesmentioning

confidence: 99%

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A partial epithelial‐mesenchymal transition signature for highly aggressive colorectal cancer cells that survive under nutrient restriction

Pastorino,

Sheraj,

Huebner

et al. 2024

The Journal of Pathology

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“…Small RNA-seq libraries were prepared by a slightly different approach than the Barcelona cohort and according to the protocol described by Gagliardi and colleagues. 38 Briefly, NEBNext Multiplex Small RNA Library Prep for Illumina (New England Biolabs, Ipswich, MA, USA) was used to process 6 μL of RNA as starting material following manufacturers’ instructions. cDNA was purified with QIAQick PCR Purification Kit (QIAGEN) after amplification.…”

Section: Methodsmentioning

confidence: 99%

SnoRNA profiling in colorectal cancer and assessment of non-invasive biomarker capacity by ddPCR in fecal samples

Gómez-Matas,

Duran-Sanchon,

Lozano

et al. 2024

iScience

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