The −44 C/G (rs1800972) polymorphism of the β‐defensin 1 is associated with increased risk of developing type 2 diabetes mellitus

Abstract

Background The aim of this study was to establish the association of two polymorphisms of the β‐defensin 1 gene (DEFB1, OMIM#602056) with the risk of developing type 2 diabetes mellitus (T2DM) in a group of Mexican patients. Methods The 5′UTR −20 G/A, and −44 C/G polymorphisms of DEFB1 gene were genotyped by 5′ exonuclease TaqMan assays in a group of 252 patients with T2DM and 522 healthy control. Results Under dominant and additive models adjusted for the risk factors, the C allele of the −44 C/G polymorphism was associated with increased risk of T2DM (OR = 1.63, 95% CI = 1.07–2.48, pCdom = 0.021 and OR = 1.42, 95% CI = 1.05–1.91, pCadd = 0.023, respectively). In addition, the linkage disequilibrium analysis showed that AC haplotype was associated with an increased risk of developing T2DM (OR = 4.39, p = 0.04). The in‐silico analysis showed that the −44 C allele produces a binding site for the transcription factor Ikaros (IK). Conclusion This study demonstrates that the C allele of −44 C/G polymorphism, as well as haplotype AC are associated with the presence of T2DM in the Mexican population. The variation in this polymorphism of the DEFB1 gene could increase the migration of the macrophages to pancreatic islets accelerate the β‐cell dysfunction in T2DM.

GHRL Gene Leu72Met Polymorphism and Type 2 Diabetes Mellitus: A Meta-Analysis Involving 8,194 Participants

“…T2DM is a multi-genic disease caused by the synergistic interactions between hereditary and environmental factors. Recently multiple genes determining the presence of T2DM have been reported as ATP binding cassette transporter 1 (ABCA 1) (2), β -defensin 1 (3), 5- lipoxygenase (ALOX5) (4), solute carrier family 30 member 8 (SLC30A8) (5), ADIPOQ, KCNJ11, TCF7L2 (6), superoxide dismutase 2 (SOD2) (7), interleukin-10 (IL-10) (8), paraoxonase 2 (PON2) gene (9), and GHRL (Ghrelin) (10). While hereditary factors play an important role in the T2DM development, clarification of their exact mechanisms depend on the identification of T2DM susceptibility genes.…”