The 245 kb amplified chromosome of Leishmania (V.) braziliensis contains a biopterin transporter gene

Sampaio, Michelle Cristina; Traub-Csekö, Yasra Maria

doi:10.1590/s0074-02762003000300014

Cited by 4 publications

(2 citation statements)

References 12 publications

(9 reference statements)

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“…However, the results obtained by Dias et al (2007) suggest that in L. braziliensis the generation of amplicons is not a common phenomenon when this specie is subjected to drug pressure. Nevertheless, our results in antimony-resistant L. braziliensis line indicate that extrachromosomal amplification of the MRPA gene may occur, corroborating the data of Sampaio and Traub-Cseko (2003). A possible explanation for the lack of amplification in the samples of L. braziliensis analyzed by Dias et al (2007) may be due to differences in the antimony-resistance level and the protocol used to obtain the resistant parasites when compared to our study.…”

Section: Discussionsupporting

confidence: 85%

“…Recently, Monte Neto et al (2011) observed that the MRPA gene is present in extrachromosomal linear amplicons in two independent mutants of antimony-resistant L. amazonensis . Sampaio and Traub-Cseko (2003) reported the presence of amplification of the biopterin transporter in a linear and stable chromosome of 245 kb in L. braziliensis (M2903 strain). However, the results obtained by Dias et al (2007) suggest that in L. braziliensis the generation of amplicons is not a common phenomenon when this specie is subjected to drug pressure.…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization of the MRPA transporter and antimony uptake in four New World Leishmania spp. susceptible and resistant to antimony

Moreira

Monte-Neto

Andrade

et al. 2013

International Journal for Parasitology: Drugs and Drug Resistan

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Section: Discussionsupporting

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Molecular characterization of the MRPA transporter and antimony uptake in four New World Leishmania spp. susceptible and resistant to antimony

Moreira

Monte-Neto

Andrade

et al. 2013

International Journal for Parasitology: Drugs and Drug Resistan

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Organization of H locus conserved repeats in Leishmania (Viannia) braziliensis correlates with lack of gene amplification and drug resistance

et al. 2007

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Resistance to antimonials is a major problem when treating visceral leishmaniasis in India and has already been described for New World parasites. Clinical response to meglumine antimoniate in patients infected with parasites of the Viannia sub-genus can be widely variable, suggesting the presence of mechanisms of drug resistance. In this work, we have compared L. major and L. braziliensis mutants selected in different drugs. The cross-resistance profiles of some cell lines resembled those of mutants bearing H locus amplicons. However, amplified episomal molecules were exclusively detected in L. major mutants. The analysis of the L. braziliensis H region revealed a strong conservation of gene synteny. The typical intergenic repeats that are believed to mediate the amplification of the H locus in species of the Leishmania sub-genus are partially conserved in the Viannia species. The conservation of these non-coding elements in equivalent positions in both species is indicative of their relevance within this locus. The absence of amplicons in L. braziliensis suggests that this species may not favour extra-chromosomal gene amplification as a source of phenotypic heterogeneity and fitness maintenance in changing environments.

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A 245 kb mini-chromosome impacts on Leishmania braziliensis infection and survival

Sampaio

Barbosa

Este

et al. 2009

Biochemical and Biophysical Research Communications

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Leishmania (V.) braziliensis, the causative agent of mucocutaneous leishmaniasis in the New World, may present an LD1 type genomic amplification that appears as a small 245 kb linear chromosome, and is not clearly associated to the presence of a selection agent. A bt1 gene, codifying for a biopterin transporter protein, was identified in this small chromosome. Leishmania are auxotrophic for pterins and one of the proposed explanations for the appearance of this amplification is the improvement of biopterin capture by the parasite. We analyzed some biological aspects of two lineages of L. braziliensis strain M2903, with and without the small amplified chromosome. We showed differences in infectivity of these lineages, in macrophages and the insect vector Lutzomyia longipalpis, as well as in the uptake and metabolization of intermediates of the Leishmania biopterin salvage pathway. Our results suggest that the genomic amplification favors survival due to improved biopterin capture and at the same time hinders the infective capability, suggesting that within a population different parasites can perform different roles.

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The 245 kb amplified chromosome of Leishmania (V.) braziliensis contains a biopterin transporter gene

Cited by 4 publications

References 12 publications

Molecular characterization of the MRPA transporter and antimony uptake in four New World Leishmania spp. susceptible and resistant to antimony

Molecular characterization of the MRPA transporter and antimony uptake in four New World Leishmania spp. susceptible and resistant to antimony

Organization of H locus conserved repeats in Leishmania (Viannia) braziliensis correlates with lack of gene amplification and drug resistance

A 245 kb mini-chromosome impacts on Leishmania braziliensis infection and survival

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