The 1H NMR Profile of Healthy Dog Cerebrospinal Fluid

Musteață, Mihai; Nicolescu, Alina; Solcan, G.; Deleanu, Călin

doi:10.1371/journal.pone.0081192

Cited by 18 publications

(17 citation statements)

References 58 publications

(59 reference statements)

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“…The most representative molecules, consistently throughout the analyzed groups, are glucose and lactate, related to energy metabolism. This finding is coherent with the data already available for dogs [ 10 ] and humans [ 32 ]. Glucose represents the most important energy supply, and, within the CNS, plays an important role in the synthesis of pivotal neurotransmitters as glutamate, GABA and aspartate [ 33 ], so that high concentrations are highly common.…”

Section: Discussionsupporting

confidence: 92%

“…Recently, canine CSF small organic molecules profile, referred to as metabolome [ 9 ], was investigated using proton nuclear magnetic resonance ( 1 H NMR) spectroscopy [ 10 ], in order to outline a fingerprint of healthy status useful for designing and interpreting clinical trials. 1 H NMR is indeed ideally tailored for metabolomics investigations on biofluids, due to its high reproducibility, its intrinsic quantitative nature and the minimum sample preparation required [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

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Age-Related 1H NMR Characterization of Cerebrospinal Fluid in Newborn and Young Healthy Piglets

et al. 2016

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When it comes to neuroscience, pigs represent an important animal model due to their resemblance with humans’ brains for several patterns including anatomy and developmental stages. Cerebrospinal fluid (CSF) is a relatively easy-to-collect specimen that can provide important information about neurological health and function, proving its importance as both a diagnostic and biomedical monitoring tool. Consequently, it would be of high scientific interest and value to obtain more standard physiological information regarding its composition and dynamics for both swine pathology and the refinement of experimental protocols. Recently, proton nuclear magnetic resonance (1H NMR) spectroscopy has been applied in order to analyze the metabolomic profile of this biological fluid, and results showed the technique to be highly reproducible and reliable. The aim of the present study was to investigate in both qualitative and quantitative manner the composition of Cerebrospinal Fluid harvested form healthy newborn (5 days old-P5) and young (30-P30 and 50-P50 days old) piglets using 1H NMR Spectroscopy, and to analyze any possible difference in metabolites concentration between age groups, related to age and Blood-Brain-Barrier maturation. On each of the analyzed samples, 30 molecules could be observed above their limit of quantification, accounting for 95–98% of the total area of the spectra. The concentrations of adenine, tyrosine, leucine, valine, 3-hydroxyvalerate, 3-methyl-2-oxovalerate were found to decrease between P05 and P50, while the concentrations of glutamine, creatinine, methanol, trimethylamine and myo-inositol were found to increase. The P05-P30 comparison was also significant for glutamine, creatinine, adenine, tyrosine, leucine, valine, 3-hydroxyisovalerate, 3-methyl-2-oxovalerate, while for the P30-P50 comparison we found significant differences for glutamine, myo-inositol, leucine and trimethylamine. None of these molecules showed at P30 concentrations outside the P05 –P50 range.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Age-Related 1H NMR Characterization of Cerebrospinal Fluid in Newborn and Young Healthy Piglets

et al. 2016

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“…One of the advantages of 1 H-NMR over other methods, is that it can generate a large quantity of information concerning the metabolic composition of samples (its spectrum represents the global profile of the analyzed sample), making possible the simultaneous identification and quantification of structurally diverse metabolites, without the need for individual isolation or no special sample preparation. [30] , [31] . The main drawback of this technique is the relatively low sensitivity.…”

Section: Introductionmentioning

confidence: 99%

MSCs Conditioned Media and Umbilical Cord Blood Plasma Metabolomics and Composition

et al. 2014

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Human mesenchymal stem cells (hMSCs) from umbilical cord (UC) blood (UCB) and matrix are tested clinically for a variety of pathologies but in vitro expansion using culture media containing fetal bovine serum (FBS) is essential to achieve appropriate cell numbers for clinical use. Human UCB plasma (hUCBP) can be used as a supplement for hMSCs culture, since UCB is rich in soluble growth factors and due to worldwide increased number of cryopreserved UCB units in public and private banks, without the disadvantages listed for FBS. On the other hand, the culture media enriched in growth factors produced by these hMSCs in expansion (Conditioned medium - CM) can be an alternative to hMSCs application. The CM of the hMSCs from the UC might be a better therapeutic option compared to cell transplantation, as it can benefit from the local tissue response to the secreted molecules without the difficulties and complications associated to the engraftment of the allo- or xeno-transplanted cells. These facts drove us to know the detailed composition of the hUCBP and CM, by 1H-NMR and Multiplexing LASER Bead Technology. hUCBP is an adequate alternative for the FBS and the CM and hUCBP are important sources of growth factors, which can be used in MSCs-based therapies. Some of the major proliferative, chemotactic and immunomodulatory soluble factors (TGF-β, G-CSF, GM-CSF, MCP-1, IL-6, IL-8) were detected in high concentrations in CM and even higher in hUCBP. The results from 1H-NMR spectroscopic analysis of CM endorsed a better understanding of hMSCs metabolism during in vitro culture, and the relative composition of several metabolites present in CM and hUCBP was obtained. The data reinforces the potential use of hUCBP and CM in tissue regeneration and focus the possible use of hUCBP as a substitute for the FBS used in hMSCs in vitro culture.

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“…The CSF analysis in dogs with spinal pathology is useful only when the results are correlated with the history, clinical findings and imaging studies (Dewey 2008;Lorenz et al 2011). To establish the diagnosis by this method is possible only in situations where microorganisms or neoplastic cells are identified in CSF samples (Musteata et al 2013;Hugo et al 2014).…”

Section: Lumbar Cerebrospinal Fluid Analysismentioning

confidence: 99%

Cauda equina syndrome in dogs - a review

et al. 2018

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A lesion of sacrococcygeal spinal nerve roots forming a structure that resembles a horse's tail results in the development of clinical entity identified as the cauda equina syndrome (CES). The disease can evolve slowly and symptomatology can be incomplete, but the fully developed CES is characterized by pain and altered sensation in the pelvic extremities, tail, perianogenital region, paresis or plegia of hind limbs, incontinence and impotence. Major causes of CES in dogs are degenerative changes of the lumbosacral vertebral column, haematoma, inflammation, neoplasm or trauma. The diagnosis is based on history, clinical presentation, neurological symptomatology, spinal röntgenography, computed tomography, and magnetic resonance imaging. In animals experiencing initial episodes of CES, conservative therapy can be attempted. But the only rational treatment of patients with severe neurological deficit is surgical decompression of the neural structures. The outcome depends on the underlying aetiology and the degree of sensory, motor and autonomic dysfunction. Canine and porcine experimental models mimicking the CES showed the involvement of intrinsic spinal cord structures. This points out the need for an early diagnosis followed by aggressive management before irreversible neuronal lesions develop. The search strategy involved the PubMed, Medline, Embase and ISI Web of Science from January 2000 to August 2017 using the terms 'cauda equina syndrome' and 'lumbosacral stenosis' in the English language literature; also references from selected papers were scanned and relevant articles included.

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The 1H NMR Profile of Healthy Dog Cerebrospinal Fluid

Cited by 18 publications

References 58 publications

Age-Related 1H NMR Characterization of Cerebrospinal Fluid in Newborn and Young Healthy Piglets

Age-Related 1H NMR Characterization of Cerebrospinal Fluid in Newborn and Young Healthy Piglets

MSCs Conditioned Media and Umbilical Cord Blood Plasma Metabolomics and Composition

Cauda equina syndrome in dogs - a review

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