The 1964 Nobel Prize for the discovery of the biosynthesis of cholesterol

Zetterström, Rolf

doi:10.1111/j.1651-2227.2009.01282.x

Cited by 4 publications

(2 citation statements)

References 20 publications

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“…Most prominent in this endeavor were Konrad Bloch and Feodor Lynen who shared the 1964 Nobel Prize in Physiology or Medicine for their discoveries (Bloch, 1965; Zetterström, 2009). Of particular importance was the identification by Bucher and Lynen of the 6-carbon compound 3-hydroxy-3-methylglutarate attached to CoA (HMG CoA) as the first intermediate committed solely to synthesis of cholesterol and other isoprenoids (Bucher et al, 1960).…”

Section: Introductionmentioning

confidence: 99%

A Century of Cholesterol and Coronaries: From Plaques to Genes to Statins

Goldstein

Brown

2015

Cell

904

670

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One-fourth of all deaths in industrialized countries result from coronary heart disease. A century of research has revealed the essential causative agent – cholesterol-carrying low density lipoprotein (LDL). LDL is controlled by specific receptors (LDLRs) in liver that remove it from blood. Mutations that eliminate LDLRs raise LDL and cause heart attacks in childhood, whereas mutations that raise LDLRs reduce LDL and diminish heart attacks. If we are to eliminate coronary disease, lowering LDL should be the primary goal. Effective means to achieve this goal are currently available. The key questions are: Who to Treat, When to Treat, and How Long to Treat.

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Section: Introductionmentioning

confidence: 99%

A Century of Cholesterol and Coronaries: From Plaques to Genes to Statins

Goldstein

Brown

2015

Cell

904

670

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“…As a major membrane lipid, cholesterol has numerous biological functions. Many human diseases such as cardiovascular diseases are correlated with enzyme defects, which may lead to the abnormal regulation of cholesterol biosynthesis and the accumulation of its intermediate products [1] , [2] , [3] . Mevalonate (MVA) pathway, a cholesterol biosynthetic pathway, is proceeded in the endoplasmic reticulum [4] .…”

Section: Introductionmentioning

confidence: 99%

Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

Wang

Sun

Sha

et al. 2015

Journal of Pharmaceutical Analysis

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A stable HMG-CoA reductase (HMGR) reaction in vitro was developed by a sensitive, selective and precise liquid chromatography–tandem mass spectrometry (LC–MS/MS) method. The optimized enzyme reaction condition contained 1.5 μg of HMGR, 20 nM of NADPH with 50 min of reaction time. The method was validated by several intra- and inter-day assays. The production transitions of m/z 147.0/59.1 and m/z 154.0/59.1 were used to detect and quantify mevalonolactone (MVAL) and MVAL-D7, respectively. The accuracy and precision of the method were evaluated over the concentration range of 0.005–1.000 μg/mL for MVAL and 0.010–0.500 μg/mL for lovastatin acid in three validation batch runs. The lower limit of quantitation was found to be 0.005 μg/mL for MVAL and 0.010 μg/mL for lovastatin acid. Intra-day and inter-day precision ranged from 0.95% to 2.39% and 2.26% to 3.38% for MVAL, 1.46% to 3.89% and 0.57% to 5.10% for lovastatin acid, respectively. The results showed that the active ingredients in Xuezhikang capsules were 12.2 and 14.5 mg/g, respectively. This assay method could be successfully applied to the quality control study of Xuezhikang capsule for the first time.

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