Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

Wang, Jing; Sun, Ji-Ye; Sha, Chunjie; Shao, Yu-Feng; Liu, Yanhong; Li, Youxin; Duan, Zhenwen; Liu, Wanhui

doi:10.1016/j.jpha.2015.06.002

Cited by 3 publications

(1 citation statement)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…HPLC and MS in combination provide easy separation with qualitative identification or qualitative analysis of crude plant samples or any other biological fluids such as plasma, urine, and blood [89] . Significantly, LC–MS is more readily used to analyze or identify polar compounds in plant samples with high accuracy and multiplexing options [90] . Higher sensitivity and specificity can be achieved by LC–MS in comparison to direct injection methods [91] .…”

Section: Advantages Of Lc–ms Methodsmentioning

confidence: 99%

Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs)

Kumar

2017

Journal of Pharmaceutical Analysis

129

View full text Add to dashboard Cite

Diets containing high proportions of fruits and vegetables reduce the risk of onset of chronic diseases. The role of herbal medicines in improving human health is gaining popularity over the years, which also increases the need for safety and efficiency of these products. Green leafy vegetables (GLVs) are the richest source of phenolic compounds with excellent antioxidant properties. Increased consumption of diets containing phenolic compounds may give positive and better results to human health and significantly improves the immune system. Highly selective, susceptible and versatile analytical techniques are necessary for extraction, identification, and quantification of phenolic compounds from plant extracts, which helps to utilize their important biological properties. Recent advances in the pre-treatment procedures, separation techniques and spectrometry methods are used for qualitative and quantitative analysis of phenolic compounds. The online coupling of liquid chromatography with mass spectrometry (LC–MS) has become a useful tool in the metabolic profiling of plant samples. In this review, the separation and identification of phenolic acids and flavonoids from GLVs by LC–MS have been discussed along with the general extraction procedures and other sources of mass spectrometer used. The review is devoted to the understanding of the structural configuration, nature and accumulation pattern of phenolic acids and flavonoids in plants and to highlighting the recent developments in the chemical investigation of these compounds by chromatographic and spectroscopic techniques. It concludes with the advantages of the combination of these two methods and prospects.

show abstract

Section: Advantages Of Lc–ms Methodsmentioning

confidence: 99%

Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs)

Kumar

2017

Journal of Pharmaceutical Analysis

129

View full text Add to dashboard Cite

show abstract

A comprehensive review of pre- and post-treatment approaches to achieve sustainable desalination for different water streams

Poirier,

Lotfi,

Garg

et al. 2023

Desalination

View full text Add to dashboard Cite

Comparison of the elution characteristics of individual forms of lovastatin in both isocratic and gradient modes and HPLC-PDA method development for pure and fermentation-derived lovastatin

Seenivasan

Gummadi

Panda

2017

Preparative Biochemistry & Biotechnology

View full text Add to dashboard Cite

The elution characteristics of lovastatin were studied by varying the composition of mobile phase in both isocratic and gradient elution modes to comprehend the role of organic modifier and acidifier on the overall analysis time and retention time of individual forms of lovastatin. Acetonitrile has influenced on the overall analysis time, whereas the acidifier determines the retention time of hydroxy acid form of lovastatin and the retention time gap between the individual forms. A combination of acetonitrile and 0.1% trifluoroacetic acid (TFA) (60:40, v/v) in isocratic elution mode eluted both hydroxy acid and lactone forms of lovastatin at 4.5 and 5.4 min, respectively. This appears to be a better approach for the separation of pharmaceutical and clinical lovastatin samples. At isocratic elution mode, a mixture of acetonitrile and either 0.05% TFA or 0.1% HPO of 60:40 (v/v) has eluted both hydroxy acid and lactone forms of lovastatin at 10 ± 0.5 and 17 ± 0.5 min, respectively. This is suitable for the fermentation-derived samples or for the complex mixtures of structural analogs. The fermentation broth (pH not adjusted) extracted with ethyl acetate at a ratio of 1:1 (v/v) at 60°C for 30 min was the optimal extraction condition for lovastatin.

show abstract

Optimization, validation and application of an assay for the activity of HMG-CoA reductase in vitro by LC–MS/MS

Cited by 3 publications

References 36 publications

Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs)

Application of HPLC and ESI-MS techniques in the analysis of phenolic acids and flavonoids from green leafy vegetables (GLVs)

A comprehensive review of pre- and post-treatment approaches to achieve sustainable desalination for different water streams

Comparison of the elution characteristics of individual forms of lovastatin in both isocratic and gradient modes and HPLC-PDA method development for pure and fermentation-derived lovastatin

Contact Info

Product

Resources

About