Thai medicinal plants modulate nitric oxide and tumor necrosis factor-α in J774.2 mouse macrophages

Punturee, Khanittha; Wild, Christopher P.; Vinitketkumneun, U.

doi:10.1016/j.jep.2004.06.019

Cited by 60 publications

(40 citation statements)

References 24 publications

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“…6) In brief, cells were incubated MTT with solution (5 mg/ml in PBS) and incubated at 37°C in 5% CO 2 for 4 h. After incubation, the plates were centrifuged at 450ϫg and supernatants were removed. Acid/isopropanol was added to the wells and mixed to dissolve completely the crystalline material.…”

Section: )mentioning

confidence: 99%

“…5) In experimental studies, H. cordata exhibited antibacterial activity, antileukemic activity, antiinflammatory activity, anaphylactic reaction and mast cell activation, and modulated nitric oxide (NO) production. [6][7][8][9][10] However, to date, studies to understand the other antibacterial effects, including the intracellularly replicating pathogens of these plants, are lacking.The mammalian immune response is divided broadly into the innate and adaptive responses. Innate immune responses of mammals involve the first line of defense against invading microorganisms, including the release of antimicrobial peptides at epithelial surfaces, phagocytosis, and intracellular killing of microorganisms by phagocytes and the activation of the complement cascade.…”

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Biological and Antibacterial Activities of the Natural Herb Houttuynia cordata Water Extract against the Intracellular Bacterial Pathogen Salmonella within the RAW 264.7 Macrophage

Kim

Lim

et al. 2008

Biological & Pharmaceutical Bulletin

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Houttuynia cordata has a long history of use in indigenous systems of medicine as an antiseptic, febrifuge, diuretic, and deobstruant. It is frequently used in many traditional medicines for its antimicrobial, antiviral, and antiinflammatory properties. [1][2][3][4] H. cordata contains of methyl nonyl ketone, caryophyllene, bornyl acetate, a-pinene, b-pinene, limonene, and other components. These components have antibacterial properties against the Gram-positive bacteria Staphylococcus aureus and Sarcina ureae. 5) In experimental studies, H. cordata exhibited antibacterial activity, antileukemic activity, antiinflammatory activity, anaphylactic reaction and mast cell activation, and modulated nitric oxide (NO) production. [6][7][8][9][10] However, to date, studies to understand the other antibacterial effects, including the intracellularly replicating pathogens of these plants, are lacking.The mammalian immune response is divided broadly into the innate and adaptive responses. Innate immune responses of mammals involve the first line of defense against invading microorganisms, including the release of antimicrobial peptides at epithelial surfaces, phagocytosis, and intracellular killing of microorganisms by phagocytes and the activation of the complement cascade. In contrast, the adaptive immune response is antigen specific and modified by previous antigen exposure. 11) In the innate immune response, the reactive oxygen intermediates (ROIs) such as superoxide and reactive nitrogen intermediates (RNIs) such as NO have a key role in intracellular killing of microorganisms within phagocytes. 12)Several studies reported that RNIs are important for controlling Salmonella multiplication within macrophages. 13,14)Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infection in animals and food-borne infection and typhoid fever in humans.15) The etiologic agents of salmonellosis are Salmonella sp. characterized by motile, Gram-negative, rod-shaped bacteria and facultative intracellular pathogens that can multiply within professional and nonprofessional phagocytes. Recently, the emergence of multidrugresistant strains of Salmonella sp. has caused more serious problems in public health.16) Multidrug resistance is a worldwide problem that does not recognize international borders and can indiscriminately affect the treatment and prevention of various diseases.To kill intracellular bacteria including Salmonella sp., Brucella sp., Listeria sp., Shigella sp., etc., much effort is needed to develop antibiotics, determine treatment duration, and resolve other issues.In this study, we examined the antibacterial effects, and macrophage activation of H. cordata water extract (HCWE) such as morphologic changes, NO production, phagocytic activity, and effects against the Salmonella enterica serovar Typhimurium in vitro and in vivo. Salmonellosis is a major bacterial zoonosis that causes a variety of disease syndromes, from self-limiting enteritis to fatal infec...

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Biological and Antibacterial Activities of the Natural Herb Houttuynia cordata Water Extract against the Intracellular Bacterial Pathogen Salmonella within the RAW 264.7 Macrophage

Kim

Lim

et al. 2008

Biological & Pharmaceutical Bulletin

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“…A number of studies have demonstrated that some Thai vegetables and herbs exhibit anti-inflammation and antioxidant activities in vivo and in vitro (Punturee et al, 2004;Nanasombat et al, 2009;Tuntipopipat et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Eryngium foetidum Suppresses Inflammatory Mediators Produced by Macrophages

Mekhora

Muangnoi²,

Chingsuwanrote

et al. 2012

Asian Pacific Journal of Cancer Prevention

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“…After 10 min, the absorbance was measured using a microplate reader (Biohit BP 800, Helsinki, Finland) at 450 nm. The NO concentration was calculated from a sodium nitrite standard curve (10,11).…”

Section: Determination Of Nitric Oxide Released From An Alveolar Macrmentioning

confidence: 99%

Factors Affecting Enhanced Permeation of Amphotericin B Across Cell Membranes and Safety of Formulation

et al. 2015

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Abstract. The aim of this study was to determine amphotericin B (AmB) permeation across lipid bilayer membranes mounted on Transwell® and to observe the phagocytosis of the AmB and the AmB-lipid formulations by alveolar macrophage (AM) cell lines using a fluorescence microscope. The lipid bilayer membranes were prepared from phospholipid and ergosterol as well as phospholipid and cholesterol in a ratio (67:33 mol%). AmB-lipid formulations were prepared from AmB incorporated with four lipid derivatives during a lyophilization process. In vitro cytotoxicity studies were carried out on kidney cells by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The levels of nitric oxide production by AMs exposed to these AmB-lipid formulations were determined by the Griess reaction. Phagocytosis of the AmB-lipid formulations was carried out using AM cells. The lipid bilayer membranes and AmB-lipid formulations were successfully prepared. In vitro cytotoxicity results showed less toxicity to kidney cells than pure AmB, and a 1,000-fold less production of nitric oxide by NR8383 cell lines was obtained when compared to lipopolysaccharide. Permeation results were two-to fivefold higher than for pure AmB in the ergosterol containing lipid bilayer and two-to fourfold higher than AmB in the cholesterol containing compositions, both of which were enough to kill the fungi according to their MICs and MFCs. AM phagocytosed the AmB-lipid formulations. We suggest that these products especially the AmB-sodium deoxycholate sulfate are potential candidates for targeting AM cells for the treatment of invasive pulmonary aspergillosis.

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Thai medicinal plants modulate nitric oxide and tumor necrosis factor-α in J774.2 mouse macrophages

Cited by 60 publications

References 24 publications

Biological and Antibacterial Activities of the Natural Herb <i>Houttuynia cordata</i> Water Extract against the Intracellular Bacterial Pathogen <i>Salmonella</i> within the RAW 264.7 Macrophage

Biological and Antibacterial Activities of the Natural Herb <i>Houttuynia cordata</i> Water Extract against the Intracellular Bacterial Pathogen <i>Salmonella</i> within the RAW 264.7 Macrophage

Eryngium foetidum Suppresses Inflammatory Mediators Produced by Macrophages

Factors Affecting Enhanced Permeation of Amphotericin B Across Cell Membranes and Safety of Formulation

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