TGF-α/HA complex promotes tympanic membrane keratinocyte migration and proliferation via ErbB1 receptor

Teh, Bing Mei; Redmond, Sharon L.; Shen, Yi; Atlas, Marcus D.; Marano, Robert J.; Dilley, Rodney J.

doi:10.1016/j.yexcr.2013.01.015

Cited by 9 publications

(3 citation statements)

References 48 publications

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“…An earlobe fat graft can achieve anatomic and functional success for small perforations, but the results are less satisfactory for large perforations [26][27][28]. Over the past decades, alternatives to resolve the limitations of conventional tympanoplasty have been widely explored [29], including the use of scaffold materials [6], bioactive molecules [5,30] and cells [31,32]. These new technologies should ideally yield more advantages than conventional tympanoplasty.…”

Section: Discussionmentioning

confidence: 99%

Comparative efficacy of platelet-rich plasma applied in myringoplasty: A systematic review and meta-analysis

Huang

Shi

et al. 2021

PLoS ONE

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Background Tympanic membrane (TM) perforation is quite common in the clinical setting. Chronic TM perforations require surgical treatments such as myringoplasty. Currently, platelet-rich plasma (PRP) is a novel, effective substance that is increasingly utilized for TM perforation repair. This study aims to evaluate the effectiveness of PRP in the application of TM perforation repair. Methods A systematic search was conducted to screen the Medline, Embase, Cochrane, Scopus and Web of Science databases up to July 2020. Studies were identified in accordance with the selection criteria by two coauthors independently. Data regarding the healing and hearing outcomes were pooled and analyzed via Review Manager version 5.3 and STATA version 12.0 software. Odds ratio (OR) was utilized to compare the closure rate. Furthermore, the results of hearing improvements and incidence of complications were also compared to evaluate the effectiveness of PRP. Results A total of eight studies with 455 participants were eligible according to the selection criteria. Compared to conventional surgery, the OR of closure was 2.70 (95% CI: 1.27 to 5.76, P = 0.01, I2 = 0%) in randomized controlled trial (RCT) subgroup and 6.18 (95% CI: 2.22 to 17.25, P = 0.0005, I2 = 0) in non-RCT subgroup. The overall OR of closure was 3.69 (95% CI: 2.02 to 6.74, P<0.0001, I2 = 0%), suggesting a significant effect on the healing of TM perforation. Between preoperative and postoperative hearing results, there is no statistical difference between the PRP and the control groups. Additionally, the use of PRP resulted in a lower incidence of complication than the use of conventional approaches. Conclusion The application of PRP during the TM surgeries can enhance the closure rate, provide similar hearing improvements and decrease the incidence of postoperative complications. Given these advantages, PRP can be considered an effective treatment for TM regeneration.

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Section: Discussionmentioning

confidence: 99%

Comparative efficacy of platelet-rich plasma applied in myringoplasty: A systematic review and meta-analysis

Huang

Shi

et al. 2021

PLoS ONE

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“…Materials and media were used as received, including milled silk powder (average particle size of 5 μm, Smiss Natural, China), chitosan powder (medium molecular weight, Sigma-Aldrich, St. Louis, MO, USA), acetic acid and ethanol (analytical grade, Chem-Supply, Australia), sodium hydroxide (Chem-Supply), two-part silicone elastomer (ACEO, Germany), Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco, MA, USA), fetal bovine serum (FBS, Bovogen, Australia), penicillin (10,000 U/mL) and streptomycin (10 000 g/mL, Aldrich), phosphate buffered saline (PBS, 10 mM, pH 7.4, Aldrich), MTS-kit bioassay (Promega Corporation), triton X-100 (Aldrich), 4,6-diamidino-2-phenylindole (DAPI, Molecular Probes), and Phalloidin Alexa568 (Molecular Probes). Human skin fibroblasts (passage number >20) were used from stock cultures . Cells were incubated in DMEM supplemented with 10% (v/v) FBS, 1% (v/v) penicillin and 1% (v/v) streptomycin in a humidified atmosphere of 5% CO 2 at 37 °C.…”

Section: Methodsmentioning

confidence: 99%

3D Printing of Silk Particle-Reinforced Chitosan Hydrogel Structures and Their Properties

Zhang

Allardyce

Rajkhowa

et al. 2018

ACS Biomater. Sci. Eng.

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Hydrogel bioprinting is a major area of focus in the field of tissue engineering. However, 3D printed hydrogel scaffolds often suffer from low printing accuracy and poor mechanical properties because of their soft nature and tendency to shrink. This makes it challenging to process them into structural materials. In this study, natural chitosan hydrogel scaffolds were, for the first time, reinforced with milled silk particles and fabricated by 3D printing. Compared with pure chitosan scaffolds, the addition of silk particles resulted in up to a 5-fold increase in compressive modulus as well as significantly better printing accuracy and improved scaffold stability. The chitosan/silk inks flowed well during printing; loading of up to 300% silk (w/w) resulted in only minor changes in the rheological properties of the ink. Particle loading also enabled tuning of the surface roughness of the scaffolds and improved scaffolds’ biodegradability. The printed composite hydrogel scaffolds showed no cytotoxicity and supported adherence and growth of human fibroblast cells.

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“…The use of cultured cells for treatment of patients with chronic otitis was reported in 1997 (Somers et al 1997), but since then, there have been no reports on clinical use. However, an increasing number of papers have described culturing of TMKs for the purpose of potential transplantation (Levin et al 2009;Mei Teh et al 2013). The rationale for cell therapy must be dependent on the presence of TM-specific stem cells, and consequently that a stem cell deficiency may prevent regeneration.…”

Section: Introductionmentioning

confidence: 99%

Transcription and microRNA Profiling of Cultured Human Tympanic Membrane Epidermal Keratinocytes

Aabel

Utheim

Olstad

et al. 2018

JARO

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The human tympanic membrane (TM) has a thin outer epidermal layer which plays an important role in TM homeostasis and ear health. The specialised cells of the TM epidermis have a different physiology compared to normal skin epidermal keratinocytes, displaying a dynamic and constitutive migration that maintains a clear TM surface and assists in regeneration. Here, we characterise and compare molecular phenotypes in keratinocyte cultures from TM and normal skin. TM keratinocytes were isolated by enzymatic digestion and cultured in vitro. We compared global mRNA and microRNA expression of the cultured cells with that of human epidermal keratinocyte cultures. Genes with either relatively higher or lower expression were analysed further using the biostatistical tools g:Profiler and Ingenuity Pathway Analysis. Approximately 500 genes were found differentially expressed. Gene ontology enrichment and Ingenuity analyses identified cellular migration and closely related biological processes to be the most significant functions of the genes highly expressed in the TM keratinocytes. The genes of low expression showed a marked difference in homeobox (HOX) genes of clusters A and C, giving the TM keratinocytes a strikingly low HOX gene expression profile. An in vitro scratch wound assay showed a more individualised cell movement in cells from the tympanic membrane than normal epidermal keratinocytes. We identified 10 microRNAs with differential expression, several of which can also be linked to regulation of cell migration and expression of HOX genes. Our data provides clues to understanding the specific physiological properties of TM keratinocytes, including candidate genes for constitutive migration, and may thus help focus further research.

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TGF-α/HA complex promotes tympanic membrane keratinocyte migration and proliferation via ErbB1 receptor

Cited by 9 publications

References 48 publications

Comparative efficacy of platelet-rich plasma applied in myringoplasty: A systematic review and meta-analysis

Comparative efficacy of platelet-rich plasma applied in myringoplasty: A systematic review and meta-analysis

3D Printing of Silk Particle-Reinforced Chitosan Hydrogel Structures and Their Properties

Transcription and microRNA Profiling of Cultured Human Tympanic Membrane Epidermal Keratinocytes

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