Tetrazolium test adjustment for wheat seeds

Abstract

-The assessment of the germination test in wheat seeds varies from 4 to 15 days, because the species normally presents dormancy in freshly harvested seeds. The tetrazolium test can characterize seed viability in less than 24 hours including lots with dormancy seeds. The objective of this study was to develop a practical and efficient procedure for evaluating the viability of wheat seeds using the tetrazolium test. Five seed lots of the BRS 208 cultivar were used, where the following were tested: a) pre-conditioning between moist paper towels or direct immersion in water for 18 hours, at 20 °C; b) longitudinal section of the embryo and the endosperm; c) coloration on paper or by immersion for 2 and 3 hours, at 30 and 40 °C; and d) concentrations of tetrazolium solution at 0.075%, 0.1%, 0.5% and 1.0%. The tetrazolium test may be efficiently used to evaluate wheat seed viability by pre-conditioning the seeds between paper towels (18 hours, at 20 °C) and adopting the following combinations of preparation and coloration: coloration of both halves of the seed on paper (2 hours, at 30 °C), in a 1.0% tetrazolium solution; or coloration of one half of the seed by immersion (3 hours, at 30 °C), in a 0.1% tetrazolium solution; or coloration of one half of the seed by immersion (2 hours, at 40 °C), in a 0.075% tetrazolium solution. This latter procedure is recommended for identifying and discarding lots with lower viability.Index terms: Triticum aestivum, germination, viability.Adequação do teste de tetrazólio para sementes de trigo RESUMO -A avaliação do teste de germinação em sementes de trigo varia de 4 a 15 dias, uma vez que a espécie normalmente apresenta dormência em sementes recém-colhidas. O teste de tetrazólio pode caracterizar a viabilidade das sementes em menos de 24 horas, inclusive para lotes com sementes dormentes. O trabalho objetivou propor procedimento prático e eficiente para avaliação da viabilidade de sementes de trigo pelo teste de tetrazólio. Utilizaram-se cinco lotes de sementes da cultivar BRS 208, testando-se: a) pré-condicionamento entre papel toalha umedecido ou por imersão direta em água, por 18 horas, a 20 °C; b) corte em bissecção longitudinal ao longo do embrião e do endosperma; c) coloração sobre papel ou por imersão, durante 2 e 3 horas, a 30 e 40 °C; e d) concentrações de solução de tetrazólio a 0,075%, 0,1%, 0,5% e 1,0%. O teste de tetrazólio pode ser empregado com eficiência para avaliação da viabilidade de sementes de trigo, por meio do pré-condicionamento das sementes entre papel (18 horas, a 20 °C), adotando-se as seguintes combinações de preparo e coloração: coloração das duas metades da semente sobre papel (2 horas a 30 °C), em solução de tetrazólio a 1,0%; ou coloração de uma das metades da semente por imersão (3 horas a 30 °C), em solução de tetrazólio a 0,1%; ou coloração de uma das metades da semente por imersão (2 horas a 40 °C), em solução de tetrazólio a 0,075%, sendo este procedimento indicado para a identificação e descarte de lotes de viabilidade inferior.Termos pa...

Morphological aspects of fruits, seeds, seedlings and in vivo and in vitro germination of species of the genus Cleome

“…This test is routinely used in quality control programs for various species (Carvalho et al, 2013). The TTC test results showed seed viability ranging from 80 to 100% and the high viability of the lots was confirmed in germination tests.…”

Increasing Seed Viability of Maize Haploid Inducing Lines by Genetic and Non‐Genetic Approaches

“…In this study, two inducer genotypes (RWS/RWK‐76 and RWK‐76/RWS) differed significantly for seed viability, total germination and speed of germination. The assessment of seed viability by TZ tests is routinely used for determining seed quality in several species, including sorghum (Noll et al, 2010), wheat (Carvalho et al, 2013), and Triticale (Souza et al, 2010). Here, 88% of the seed of RWS/RWK‐76 were viable as compared with 41% in RWK‐76/RWS.…”

TETRAZOLIUM TEST TO ESTIMATE OKRA (Abelmoschus esculentus (L.) MOENCH) SEED VIABILITY

“…The tetrazolium test has been used to assess the physiological quality of various species, such as soybean (FRANÇA-NETO; KRYZANOWSKI; COSTA, 1998), Brachiaria (NOVEMBRE; CHAMMA; GOMES, 2006), maize (CHAMMA; , cotton (CERVI; MENDONÇA, 2009), sunflower (SILVA et al, 2013) and wheat (CARVALHO et al, 2013), because of its quality and fast results. Among vegetables, this test has been used to assess the viability of seeds of zucchini (BARROS et al, 2005), tomato (SANTOS; NOVEMBRE; MARCOS FILHO, 2007), watermelon (NERY; CARVALHO; OLIVEIRA, 2007) and cucumber (LIMA; PINTO; NOVEMBRE, 2010).…”