Tetraspanin 1 is involved in survival, proliferation and carcinogenesis of pancreatic cancer

Hou, Feng-Qiang; Lei, Xi-Feng; Yao, Jian-Long; Wang, Yijin; Zhang, Wei

doi:10.3892/or.2015.4272

Cited by 31 publications

(28 citation statements)

References 25 publications

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“…TSPANs are a large family of four-pass transmembrane proteins that are ubiquitously expressed in mammals, and emerging evidence supports their crucial role in human cancer [32]. Since TSPANs were first identified in human leukocytes, numerous studies reported that their expression correlates with tumor type, stage, and clinical outcome of patients [33].…”

Section: E C Bmentioning

confidence: 99%

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC)

Dong

Wang

2018

Bosn J of Basic Med Sci

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Previous studies demonstrated dysregulation of different microRNAs in thyroid cancer. Tetraspanins (TSPANs) are cell surface proteins with critical roles in many cellular processes, and implications in tumor development. Here we investigated the role of miR-369-3p in papillary thyroid cancer (PTC) and its association with TSPAN13. miR-369-3p and the TSPAN13 gene expression profiles of 513 thyroid cancer and 59 normal thyroid tissues were downloaded from the Cancer Genome Atlas database. Thyroid cancer tissues were classified according to the histological type, grouped based on low and high median miR-369-3p and TSPAN13 expression, and analyzed in relation to overall survival (OS) of patients. Human PTC cell lines (TPC-1 and GLAG-66) and human embryonic kidney 293T (HEK293T) cells were used for in vitro analysis. Transfection experiments were performed with synthetic miRNA mimics for miR-369-3p and small interfering RNAs for TSPAN13. Relative expression of miR-369-3p and TSPAN13 mRNA was determined by RT-qPCR. Protein levels of TSPAN13 were determined by western blotting. Cell proliferation (CCK-8 assay), colony formation, and apoptosis (flow cytometry) were analyzed in transfected cells. Binding sites of miR-369-3p in TSPAN13 mRNA were determined by bioinformatics analysis and dual luciferase reporter assay. miR-369-3p was downregulated and TSPAN13 upregulated in PTC, follicular thyroid cancer, and tall cell variant tissues. Both low expression of miR-369-3p and high expression of TSPAN13 were associated with shorter OS in thyroid cancer patients. Overexpression of miR-369-3p significantly suppressed proliferation and promoted apoptosis in PTC cells. TSPAN13 was a direct target of miR-369-3p, and silencing of TSPAN13 phenocopied the effect of miR-369-3p mimics in PTC cells. Overall, the downregulation of miR-369-3p and consequent upregulation of its target TSPAN13 appear to be involved in pathophysiology of PTC.

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Section: E C Bmentioning

confidence: 99%

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC)

Dong

Wang

2018

Bosn J of Basic Med Sci

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“…Quantitative PCR of miRNA was performed using an Applied Biosystems 7300 Sequence Detection system. The 10 µl PCR reaction contained 0.67 µl reverse transcription product, 1X TaqMan Universal PCR Master Mix, and 1 µl of the primer and probe mix, according to the TaqMan MicroRNA assay protocol (Applied Biosystems) (16). The relative expression levels of genes were determined using the 2 -∆∆Ct analysis method.…”

Section: Sample Preparationmentioning

confidence: 99%

miR-768-3p is involved in the proliferation, invasion and migration of non-small cell lung carcinomas

Xie

Chen

et al. 2017

International Journal of Oncology

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Altered microRNA expression has been found to be a common feature of several cancers, including lung carcinomas. However, the possible roles of miR-768-3p in the pathological changes of lung carcinomas are still unknown. The aim of the present study was to investigate the expression and possible effects of miR-768-3p in human non-small cell lung carcinomas (NSCLC). Eighty-three NSCLC patients attending the clinic of Kunming Hospital were invited to participate in the study. Their tumor samples were obtained for qRT-PCR analysis. Human NSCLC cell lines, A549 and HCC4006, were employed and transfected with either miR-768-3p mimics or miR-768-3p antagomir. Following transfection, the in vitro and in vivo proliferation, apoptosis fractions, migration and invasion of NSCLC cells were evaluated. The data revealed that: i) upregulated miR-768-3p in tumors were associated with the clinicopathological features of NSCLC patients; ii) inhibiting miR-768-3p function by miR-768-3p antagomir induced distinctly apoptosis and Fas/FasL expressional alteration of NSCLC cells; iii) miR-768-3p antagomir transduction also decreased the viability, migration and invasion, as well as MMP-2 and MMP-9 activities in A549 and HCC4006 cells; and iv) miR-768-3p antagomir transfection also inhibited the growth and proliferation of NSCLC xenografts in nude mice. The present results suggested that abnormal elevated miR-768-3p in NSCLC tumors and cell lines played important roles in NSCLC carcinogenic progression, and the targeting of miR-768-3p might be a potential therapeutic strategy for the treatment of NSCLC.

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“…Characterization of these mutants revealed an involvement of plant TETs in the control of cell proliferation, cell differentiation and cell identity, as well as tissue patterning. Such developmentally important functions have been described for tetraspanins earlier in other model organisms (Kazarov et al, 2002; Hemler, 2008; Franco et al, 2010; Anderson et al, 2011; Hou et al, 2015), suggesting that TET functions are partially conserved between kingdoms.…”

Section: Tetraspanin Mutants In Plantsmentioning

confidence: 53%

TETRASPANINs in Plants

2017

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Tetraspanins are small transmembrane proteins that laterally associate with each other and cluster with numerous partner proteins as well as lipids. These interactions result in the formation of a distinct class of membrane domains, the tetraspanin-enriched microdomains (TEMs), which influence numerous cellular processes such as cell adhesion and fusion, intracellular membrane trafficking, signaling, morphogenesis, motility as well as interaction with pathogens and cancer development. The majority of information available about tetraspanins is based on studies using animal models or cell lines, but tetraspanins are also present in fungi and plants. Recent studies indicate that tetraspanins have important functions in plant development, reproduction and stress responses. Here we provide a brief summary of the current state of tetraspanin research in plants.

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Tetraspanin 1 is involved in survival, proliferation and carcinogenesis of pancreatic cancer

Cited by 31 publications

References 25 publications

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC)

Downregulation of TSPAN13 by miR-369-3p inhibits cell proliferation in papillary thyroid cancer (PTC)

miR-768-3p is involved in the proliferation, invasion and migration of non-small cell lung carcinomas

TETRASPANINs in Plants

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