Tetracycline and its analogues protect Caenorhabditis elegans from β amyloid-induced toxicity by targeting oligomers

Multivariate Analysis for Fourier Transform Infrared Spectra of Complex Biological Systems and Processes

“…For these reasons, it became recently a very attracting tool for biomedical research [20,[22][23][24], being successfully employed for the study of several biological systems, from intact cells [6,7,25] to tissues [11,26,27] and whole model organisms (i.e. the nematode Caenorhabditis elegans) [9,28].…”

“…In this way, FTIR microspectroscopy provides detailed information on several biological processes in situ, among which stem cell differentiation [1][2][3][4][5], somatic cell reprogramming [6], cell maturation [7,8], amyloid aggregation [9][10][11][12] and cancer onset and progression [13][14][15], making it possible to disclose the infrared response not only from single cells, but also from subcellular compartments [8,16,17].…”

Supplementation of S-allyl cysteine improves health span in Caenorhabditis elegans

“…Using the same C. elegans genetic AD animal model, an insulin/IGF-1-like signal modulates anti-oxidative responses and lifespan and ameliorates toxicity caused by the human Aβ transgene (COHEN et al, 2006). Supplementation of tetracycline significantly retards paralysis by the expression of Aβ (DIOMEDE et al, 2010). These findings suggest that SAC has preventive activity against Aβ toxicity and can be a strong candidate compound for the development of therapeutic agents for AD.…”

Fourier Transform Infrared Microspectroscopy as a Tool for Embryonic Stem Cell Studies

“…For these reasons, a spectrum of good quality can be collected by an area larger than 20 μm x 20 μm -when using a nitrogen cooled Mercury Cadmium Telluride (MCT) detector (Orsini et al, 2000). Thanks to the variable aperture of the microscope, it is possible to select a small area of few tens of microns within the sample, enabling the study of intact cells (Tanthanuc et al, 2010;Thumanu et al, 2009;Ami et al, 2008;Wood et al, 2008), tissues (Choo et al, 1996) and whole model organisms, as nematodes (Diomede et al, 2010;Ami et al, 2004). Instead, more advanced IR microscopes employ a focal plane array (FPA) detection to collect the IR chemical imaging of the sample, with a spatial resolution improved compared to that of the conventional microscope.…”

“…The potential of FTIR and Raman spectroscopies has been widely exploited to monitor biological processes in-situ, for instance in cancer diagnosis (Krafft et al, 2009;Baker et al, 2008;Wang et al, 2007), in protein aggregation (Diomede et al, 2010;Doglia et al, 2008;Ami et al, 2005;Choo et al, 1996) and in stem cell research Heraud & Tobin, 2009;Ami et al, 2008;Notingher et al, 2004a;Notingher et al, 2004b). Indeed, these vibrational approaches have been shown to be a promising tool for the characterization of cellular mechanisms, providing not only structural information, but also details on the dynamics of the structures (Miller & Dumas, 2010).…”