Tetra-functional multilayer coatings for cardiovascular stent materials

Saadatlou, Ghazaleh Azizi; Ijaz, Aatif; Sipahioğlu, Dilara; Surme, Saliha; Kavaklı, İbrahim Halil; Gurpinar, Yunus; Yalçın, Özlem; Motallebzadeh, Amir; Güner, Pınar Tatar

doi:10.1016/j.colsurfa.2023.131571

Cited by 7 publications

(4 citation statements)

References 53 publications

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“…Blood clot formation time was shown to increase significantly after the substrates were coated with 5 bilayers. In a previous study by our group, LbL coatings of partially hydrolyzed poly(2-ethyl-2-oxazoline) and heparin on 316L stainless steel or nitinol alloys were shown to decrease the relative coagulation rate by approximately 50% compared to uncoated substrates 14 . In another study, LbL coatings of PEI, epigallocatechin gallate (EGCG), or tannic acid with heparin on polydopamine modified polylactic acid surfaces were investigated for their hemocompatibility.…”

Section: Introductionmentioning

confidence: 88%

“…With increasing hydrolysis degree of the copolymers, a bigger frequency shift indicating larger amounts of deposited molecules was observed. Increase in the hydrolysis degree results in the formation of more positively charged amine groups on the backbone of the polymer 14 . As binding sites for the polyanionic https://doi.org/10.26434/chemrxiv-2024-q4tws ORCID: https://orcid.org/0000-0003-0984-0375 Content not peer-reviewed by ChemRxiv.…”

Section: Growth Of Peox-co-pei/heparin Multilayersmentioning

confidence: 99%

“…Dry copolymers were obtained by freeze drying at 0.08 mbar with Labconco Freezone lyophilizer. The resulting copolymers (PEOX-co-PEI) were characterized by NMR to determine the hydrolysis degree (number of monomers hydrolyzed in a chain) 14 . A hydrolysis degree range of 5% to 60% was obtained by this procedure.…”

Section: Copolymer Preparationmentioning

confidence: 99%

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Control of Heparin Surface Density in Multilayers of Partially Hydrolyzed Poly(2-ethyl-2-oxazoline) by Degree of Hydrolysis

Azizi Saadatlou,

Tatar Guner,

Demirel

2024

Preprint

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Controlling the surface density of heparin in active anticoagulant coatings is important in applications where an optimum is required. An approach based on tuning the degree of hydrolysis of poly(2-ethyl-2-oxazoline) (PEOX) is presented to control the surface density of heparin in layer-by-layer (LbL) assembled films. Multilayers are prepared at pH5 in 0.5M aqueous NaCl solutions by electrostatic interactions between negatively charged heparin and the positively charged amine groups in hydrolyzed PEOX. Characterization of the multilayers by QCM-D, TBO assay and XPS all show that the amount of heparin deposited increases with the degree of hydrolysis. While non-hydrolyzed PEOX/Heparin multilayers do not grow, the average deposited mass per area per bilayer, as determined by QCM-D measurements, increases with the degree of hydrolysis. At 50% hydrolysis, ITC measurements exhibit an exothermic enthalpy below -500 kJ/mol, TBO assay gives a heparin surface density of 1.03 ug/cm2 and atomic % of sulfur as determined by XPS leveled off at ~14%. These results show the potential of acidic hydrolysis of PEOX combined with LbL assembly of heparin as a reproducible method for controlling the surface density of heparin in anticoagulant coatings.

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Section: Introductionmentioning

confidence: 88%

Section: Growth Of Peox-co-pei/heparin Multilayersmentioning

confidence: 99%

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Control of Heparin Surface Density in Multilayers of Partially Hydrolyzed Poly(2-ethyl-2-oxazoline) by Degree of Hydrolysis

Azizi Saadatlou,

Tatar Guner,

Demirel

2024

Preprint

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“…The construction of polymeric drug-loaded coatings on the surface of nickel–titanium alloy stents is a promising modification strategy. ,, However, the introduced polymers are usually complex in composition, and their continuous degradation can bring about serious inflammatory reactions. − At the same time, the toxicity of the drug itself can also bring potential toxic side effects to normal organs with a blood circulation. Therefore, the development of polymer-free coatings with selective cancer inhibition and antimicrobial properties is a promising research direction.…”

Section: Introductionmentioning

confidence: 99%

Selective Tumor Inhibition Effect of Drug-Free Layered Double Hydroxide-Based Films via Responding to Acidic Microenvironment

Xing,

Zhang,

Liu

et al. 2024

ACS Biomater. Sci. Eng.

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Nickel−titanium alloy stents are widely used in the interventional treatment of various malignant tumors, and it is important to develop nickel−titanium alloy stents with selective cancer-inhibiting and antibacterial functions to avoid malignant obstruction caused by tumor invasion and bacterial colonization. In this work, an acid-responsive layered double hydroxide (LDH) film was constructed on the surface of a nickel−titanium alloy by hydrothermal treatment. The release of nickel ions from the film in the acidic tumor microenvironment induces an intracellular oxidative stress response that leads to cell death. In addition, the specific surface area of LDH nanosheets could be further regulated by heat treatment to modulate the release of nickel ions in the acidic microenvironment, allowing the antitumor effect to be further enhanced. This acid-responsive LDH film also shows a good antibacterial effect against S. aureus and E. coli. Besides, the LDH film prepared without the introduction of additional elements maintains low toxicity to normal cells in a normal physiological environment. This work offers some guidance for the design of a practical nickel−titanium alloy stent for the interventional treatment of tumors.

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Control of heparin surface density in multilayers of partially hydrolyzed poly(2‐ethyl‐2‐oxazoline) by degree of hydrolysis

Saadatlou,

Guner,

Demirel

2024

Journal of Polymer Science

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Controlling the surface density of heparin in active coatings is important in providing anticoagulation while preventing bleeding. An approach based on tuning the degree of hydrolysis of poly(2‐ethyl‐2‐oxazoline) (PEOX) is presented to control the surface density of heparin in layer‐by‐layer (LbL) assembled films. Multilayers are prepared at pH 5 in 0.5 M aqueous NaCl solutions by electrostatic interactions between negatively charged heparin and the positively charged amine groups in hydrolyzed PEOX. Characterization of the multilayers by quartz crystal microbalance with dissipation (QCM‐D), toluidine blue (TBO) assay and X‐ray Photoelectron Spectroscopy (XPS) all shows that the amount of heparin deposited increases with the degree of hydrolysis. While non‐hydrolyzed PEOX/heparin multilayers do not grow, the average deposited mass per area per bilayer as determined by QCM‐D measurements increases with the degree of hydrolysis. At 50% hydrolysis, TBO assay gives a heparin surface density of 1.03 μg/cm2 and atomic % of sulfur as determined by XPS leveled off at ~14%. These results show the potential of acidic hydrolysis of PEOX combined with LbL assembly of heparin as a reproducible method for controlling the surface density of heparin in anticoagulant coatings.

show abstract

Tetra-functional multilayer coatings for cardiovascular stent materials

Cited by 7 publications

References 53 publications

Control of Heparin Surface Density in Multilayers of Partially Hydrolyzed Poly(2-ethyl-2-oxazoline) by Degree of Hydrolysis

Control of Heparin Surface Density in Multilayers of Partially Hydrolyzed Poly(2-ethyl-2-oxazoline) by Degree of Hydrolysis

Selective Tumor Inhibition Effect of Drug-Free Layered Double Hydroxide-Based Films via Responding to Acidic Microenvironment

Control of heparin surface density in multilayers of partially hydrolyzed poly(2‐ethyl‐2‐oxazoline) by degree of hydrolysis

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