Tethered non-ionic micelles: a matrix for enhanced solubilization of lipophilic compounds

Patchornik, Guy; Namboothiri, Irishi N. N.; Nair, Divya K.; Wachtel, Ellen; Persky, Rachel

doi:10.1039/c2sm25708d

Cited by 17 publications

(20 citation statements)

References 28 publications

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“…The General Procedure of the Triton-X based MCs formation procedures is described in [1] and is based on the works [2] , [3] , [4] , [5] , [6] , [7] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on peptidyl platform-based anticancer drug synthesis and triton-x-based micellar clusters (MCs) self-assembly peculiarities for enhanced solubilization, encapsulation of hydrophobic compounds and their interaction with HeLa cells

et al. 2019

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The data presented here refer to a research article entitled “Self-Assembled Micellar Clusters Based on Triton-X-family Surfactants for Enhanced Solubilization, Encapsulation, Proteins Permeability Control, and Anticancer Drug Delivery” Solomonov et al., 2019. The present article provides the General Procedure for clusterization of Triton-X-based micelles and the effect of (i) metal ion, surfactant, and chelator concentration on the developed clusters formation, (ii) surfactant-chelator relation change, (iii) metal ion-micelles concertation ratio variation, (iv) metal ion replacement, (v) solvent replacement, (vi) kinetics of clusters formation, (vii) hydrophobic fluorescent dye (Coumarin 6) solubilization in aqueous MCs media, (viii) novel anticancer peptidyl drug synthesis and characterization and (ix) the viability of HeLa cells with and without the presence of drug-free Triton-X-based family MCs. These data provide additional insights useful for understanding all aspects of the micellar clusters formation, optimization, and control.

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“…The General Procedure of the Triton-X based MCs formation procedures is described in [1] and is based on the works [2] , [3] , [4] , [5] , [6] , [7] .…”

Section: Experimental Design Materials and Methodsmentioning

confidence: 99%

Data on peptidyl platform-based anticancer drug synthesis and triton-x-based micellar clusters (MCs) self-assembly peculiarities for enhanced solubilization, encapsulation of hydrophobic compounds and their interaction with HeLa cells

et al. 2019

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“…However, we have found that it is possible to exploit the micellar moiety for a new objective, i.e., directing PDCs to assemble via specifically conjugated micelles under controlled conditions. Such an objective builds on recent studies that have shown how a variety of amphiphilic [metal:chelator] complexes presumably located at the micelle/water interface, are able to promote large-scale micellar conjugation 22 , 23 . Here we demonstrate that successful implementation of this approach on PDCs (outlined in the cartoon in Fig.…”

Section: Introductionmentioning

confidence: 99%

Promoting crystallization of intrinsic membrane proteins with conjugated micelles

Truong

Ghosh

Wachtel

et al. 2020

Sci Rep

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A new technique for promoting nucleation and growth of membrane protein (MP) crystals from micellar environments is reported. It relies on the conjugation of micelles that sequester MPs in protein detergent complexes (PDCs). Conjugation via amphiphilic [metal:chelator] complexes presumably takes place at the micelle/water interface, thereby bringing the PDCs into proximity, promoting crystal nucleation and growth. We have successfully applied this approach to two light-driven proton pumps: bacteriorhodopsin (bR) and the recently discovered King Sejong 1–2 (KS1–2), using the amphiphilic 4,4′-dinonyl-2,2′-dipyridyl (Dinonyl) (0.7 mM) chelator in combination with Zn 2+ , Fe 2+ , or Ni 2+ (0.1 mM). Crystal growth in the presence of the [metal–chelator] complexes leads to purple, hexagonal crystals (50–75 µm in size) of bR or pink, rectangular/square crystals (5–15 µm) of KS1–2. The effects of divalent cation identity and concentration, chelator structure and concentration, ionic strength and pH on crystal size, morphology and process kinetics, are described.

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“…First, the hydrophobic chelator, bathophenanthroline (batho), is added to a dispersion of Tween-20 micelles and transform the latter into what we have called 'Engineeredmicelles'. [15][16][17] These contain, in addition to the detergent, a hydrophobic chelator that positions itself at the micelle/water interface ( Figure 1). In the following step, Fe 2+ ions are added as FeSO 4 .…”

Section: Introductionmentioning

confidence: 99%

A general platform for antibody purification utilizing engineered-micelles

Dhandapani

Howard

Truong

et al. 2019

mAbs

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We introduce a new concept and potentially general platform for antibody (Ab) purification that does not rely on chromatography or specific ligands (e.g., Protein A); rather, it makes use of detergent aggregates capable of efficiently capturing Ab while rejecting hydrophilic impurities. Captured Ab are then extracted from the aggregates in pure form without co-extraction of hydrophobic impurities or aggregate dissolution. The aggregates studied consist of conjugated "Engineered-micelles" built from the nonionic detergent, Tween-20; bathophenanthroline, a hydrophobic metal chelator, and Fe 2+ ions. When tested in serum-free media with or without bovine serum albumin as additive, human or mouse IgGs were recovered with good overall yields (70-80%, by densitometry). Extraction of IgGs with 7 different buffers at pH 3.8 sheds light on possible interactions between captured Ab and their surrounding detergent matrix that lead to purity very similar to that obtained via Protein A or Protein G resins. Extracted Ab preserve their secondary structure, specificity and monomeric character as determined by circular dichroism, enzyme-linked immunosorbent assay and dynamic light scattering, respectively.

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Tethered non-ionic micelles: a matrix for enhanced solubilization of lipophilic compounds

Cited by 17 publications

References 28 publications

Data on peptidyl platform-based anticancer drug synthesis and triton-x-based micellar clusters (MCs) self-assembly peculiarities for enhanced solubilization, encapsulation of hydrophobic compounds and their interaction with HeLa cells

Data on peptidyl platform-based anticancer drug synthesis and triton-x-based micellar clusters (MCs) self-assembly peculiarities for enhanced solubilization, encapsulation of hydrophobic compounds and their interaction with HeLa cells

Promoting crystallization of intrinsic membrane proteins with conjugated micelles

A general platform for antibody purification utilizing engineered-micelles

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