Tethered-bead, immune sandwich assay

Silver, Jonathan; Li, Zhenyu; Neuman, Keir C.

doi:10.1016/j.bios.2014.07.011

Cited by 10 publications

(10 citation statements)

References 21 publications

(21 reference statements)

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“…Detectable signal amplification and noise reduction are one of the most important strategies in lowering the detection limit and increasing the sensitivity of the assay method, particularly immunoassay development 1 2 . Typically, natural enzymes including horseradish peroxidase and alkaline phosphatase have shown great application potentials because of their unique advantages: high catalytic activity, high specificity and easy conjugation with proteins 3 4 5 . Despite these extensive developments, natural enzymes often suffer from inherent shortcomings such as high cost of preparation and purification, low operational stability, sensitivity of catalytic activity to environmental conditions and difficulties in recovering 6 .…”

mentioning

confidence: 99%

Cobalt-Porphyrin-Platinum-Functionalized Reduced Graphene Oxide Hybrid Nanostructures: A Novel Peroxidase Mimetic System For Improved Electrochemical Immunoassay

Shu

Qiu

Wei

et al. 2015

Sci Rep

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5,10,15,20-Tetraphenyl-21H,23H-porphine cobalt flat stacking on the reduced graphene oxide with platinum nanoparticles (PtNPs/CoTPP/rGO) were first synthesized and functionalized with monoclonal rabbit anti-aflatoxin B1 antibody (anti-AFB1) for highly efficient electrochemical immunoassay of aflatoxin B1 (AFB1) in this work. Transmission electron microscopy (TEM), atomic force microscope (AFM) and spectral techniques were employed to characterize the PtNPs/CoTPP/rGO hybrids. Using anti-AFB1-conjugated PtNPs/CoTPP/rGO as the signal-transduction tag, a novel non-enzymatic electrochemical immunosensing system was designed for detection of target AFB1 on the AFB1-bovine serum albumin-functionalized sensing interface. Experimental results revealed that the designed immunoassay could exhibit good electrochemical responses for target analyte and allowed the detection of AFB1 at a concentration as low as 5.0 pg mL−1 (5.0 ppt). Intra- and inter-assay coefficients of variation were below 10%. Importantly, the methodology was further validated for analyzing naturally contaminated or spiked blank peanut samples with consistent results obtained by AFB1 ELISA kit, thus providing a promising approach for quantitative monitoring of organic pollutants.

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mentioning

confidence: 99%

Cobalt-Porphyrin-Platinum-Functionalized Reduced Graphene Oxide Hybrid Nanostructures: A Novel Peroxidase Mimetic System For Improved Electrochemical Immunoassay

Shu

Qiu

Wei

et al. 2015

Sci Rep

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“…The motion of tethered beads in flow is recognizable with a low-power optical microscope and distinguishable from that of beads that stick nonspecifically to the surface. Recently, a proof-of-principle immune sandwich assay was described for detection of single immune complexes based on the above principle [55].…”

Section: Surface Functionalization Of Microbeadsmentioning

confidence: 99%

Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

Yue

Yang

2016

Microfluidic Methods for Molecular Biology

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Early screening and diagnosis of diseases require the development of sensitive, reliable, and inexpensive high-throughput assays. There is a growing need for innovative diagnostic technologies that provide accurate detection of a broad range of diseases and enhance laboratory productivity. Developments in micro-and nanotechnology have advanced the "lab-on-a-chip" concept towards a new generation of point-of-care diagnostic devices that enable parallel detection of multiple analytes in small-volume samples with high sensitivity in a short time. These features fulfill some of the important criteria of bioanalysis used for biochemical studies, environmental analyses, and clinical diagnostics. However, the multiplexing capability of microfluidic-based assay is limited compared to conventional flow cytometric assays, particularly for encoded microbead-based assays, which are capable of simultaneously analyzing multiple analyte. It is possible to incorporate the microbead-based assays into microfluidic devices in order to retain all the advantages of the microdevice and significantly improve multiplexing capability. In this chapter, we summarize the latest development in microbeadbased assays and discuss the integration of the microbead technology with microfluidic devices. Applications of the integrated approach in multiplexed protein and DNA analysis are growing rapidly in the areas of biomarker research, cancer screening, and disease diagnosis. The prospects for future development and commercialization of these microbead-based microfluidic devices are also discussed.

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“…Silver et al [137] developed asandwich immunoassay for the detection of PSA based on tethered particle monitoring. Magnetic beads (2.8 mm) with immobilized antibody captured Figure 16.…”

Section: Dark-field Microscopymentioning

confidence: 99%

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

et al. 2020

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The ability to detect low concentrations of analytes and in particular low‐abundance biomarkers is of fundamental importance, e.g., for early‐stage disease diagnosis. The prospect of reaching the ultimate limit of detection has driven the development of single‐molecule bioaffinity assays. While many review articles have highlighted the potentials of single‐molecule technologies for analytical and diagnostic applications, these technologies are not as widespread in real‐world applications as one should expect. This Review provides a theoretical background on single‐molecule—or better digital—assays to critically assess their potential compared to traditional analog assays. Selected examples from the literature include bioaffinity assays for the detection of biomolecules such as proteins, nucleic acids, and viruses. The structure of the Review highlights the versatility of optical single‐molecule labeling techniques, including enzymatic amplification, molecular labels, and innovative nanomaterials.

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Tethered-bead, immune sandwich assay

Cited by 10 publications

References 21 publications

Cobalt-Porphyrin-Platinum-Functionalized Reduced Graphene Oxide Hybrid Nanostructures: A Novel Peroxidase Mimetic System For Improved Electrochemical Immunoassay

Cobalt-Porphyrin-Platinum-Functionalized Reduced Graphene Oxide Hybrid Nanostructures: A Novel Peroxidase Mimetic System For Improved Electrochemical Immunoassay

Microfluidics for DNA and Protein Analysis with Multiplex Microbead-Based Assays

Advances in Optical Single‐Molecule Detection: En Route to Supersensitive Bioaffinity Assays

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