tetA and tetB Genes in Klebsiella Pneumoniae Isolated From Clinical Samples

Abstract

Background:The emergence of antibiotic resistance among clinical and nonclinical bacteria is a global public health problem. Klebsiella Pneumoniae is one of the most pathogens that contains a variety of gens and shows resistance to many antibiotics. Perpetual monitoring of the resistant bacteria is an important in order to limit the development of resistance among these pathogens. Objectives: The current study aimed to monitor the prevalence of tetA and tetB resistance genes in Klebsiella Pneumoniae species isolated from the patients with urinary tract infection who hospitalized in Mir Hospital of Zabol, Iran from 2011 to 2012. Materials and Methods: In the present cross-sectional study, a total of 30 strains of K. pneumoniae were isolated from urine cultures of hospitalized patients in Mir Hospital (Zabol, south-east of Iran) who had urinary tract infections from 2011 to 2012. Antibiotic susceptibility of isolates was evaluated for four antibiotics including ceftazidime, cefixime, tetracycline and erythromycin using standard Kirby-Bauer disk diffusion method. The K. pneumoniae genome was extracted by simple boiling method, and polymerase chain reaction (PCR) method also was used to detect tetA and tetB genes by specific pair of primers. Results: The K. pneumonia isolates were resistant to erythromycin (70%), cefixime (53.3%), tetracycline (50%) and ceftazidime (36.6%). The amplification of tetA and tetB genes of K. pneumonia revealed that all of the isolates harbored these genes. Conclusions: Resistance to tetracycline and other antibiotics, and the presence of various resistance genes in K. pneumonia strains are alarming signs in Zabol area. The current study strongly recommends limiting the consumption of antibiotics including tetracycline. Further studies should be conducted in order to find out the extent of the problem in other areas.

Molecular identification of antibiotic-resistant bacteria isolated from used contact lens cases

“…According to Cumurcu et al (2011), a case was reported where K. pneumoniae was identified in an eye infection associated to CL wear from corneal scrap of a 19 years old boy, where the CL worn by the patient with an extended period. A study by Bokaeian et al (2014) also supports the finding in this study where 70% of K. pneumoniae isolated was also resistant towards erythromycin. The emergence of this antibiotic-resistant bacteria might be due to the ability of this bacteria to encoded a gene called erythromycin ribosome methylase (erm), where it leads to methylation of antibiotic ribosomal target and impaired the binding ability of the erythromycin to its target site (Depardieu et al, 2007;Sun et al, 2018).…”

Prevalence of Tetracycline Resistance Genes tet (A, B, C, 39) in Klebsiella pneumoniae Isolated from Tehran, Iran

“…The study by Bokaeian et al (2014) was conducted on 30 samples of K. pneumoniae. The results of this group showed that the isolates were resistant to erythromycin (70%), cefixime (53%), tetracycline (50%), and ceftazidime (36%).…”

Revisiting ESKAPE Pathogens: virulence, resistance, and combating strategies focusing on quorum sensing

“…Tet(K) and Tet(L) are expressed in Gram-positive pathogens, which are antiporters of monovalent H + having 14 transmembrane segments of α and β domains. In both K. pneumoniae and A. baumannii , tetA and tetB are present and encode the H + antiporters having 12 transmembrane segments of α and β domains ( Bokaeian et al., 2014 ). Tet(A) and Tet(B) are present mainly in Gram-negative pathogens ( Maleki et al., 2014 ).…”