Testosterone-induced permanent changes of hepatic gene expression in female mice sustained during Plasmodium chabaudi malaria infection

Delić, Denis; Gailus, Nicole; Vohr, Hans-Werner; Dkhil, Mohamed A.; Al‐Quraishy, Saleh; Wunderlich, Frank

doi:10.1677/jme-10-0026

Cited by 28 publications

(10 citation statements)

References 56 publications

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“…Hyperprolactinemia Did Not Influence the Serum Levels of GH or TT. Since expression of these sex-specific hepatic genes is regulated by GH, and some of them are also affected by testosterone (Deli c et al, 2010;Knopf et al, 1983), we verified the influence of hyperprolactinemia on serum levels of GH and TT. In both male and female mice treated with pCAGGS-mPrl, the serum levels of GH were not significantly Fig.…”

Section: Resultsmentioning

confidence: 90%

Prolactin Upregulates Female-PredominantP450Gene Expressions and Downregulates Male-Predominant Gene Expressions in Mouse Liver

et al. 2017

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Prolactin is a polypeptide hormone with over 300 separate biologic activities. Its serum level is increased during pregnancy and lactation, and it has been reported that pregnancy and lactation affect drug and steroid metabolism in mice and humans. Several studies reported that pregnancy or lactation influences liver cytochrome P450 (P450) expression and its activity, affecting the biosynthesis of steroids and xenobiotics through growth hormone or sex hormones; however, the role of prolactin as the regulator of liver expression has not been elucidated so far. In the present study, we focused on prolactin as the regulator of expression of liver sex-predominant genes, including To investigate the role of prolactin in the hepatic gene expressions, pCAGGS expression vector containing mouse prolactin cDNA was transfected by hydrodynamic injection into both male and female mice. Hyperprolactinemia phosphorylated signal transducer and activator of transcription 5 in the liver and augmented female mouse liver mRNA expression of ,, ,, and prolactin receptor genes, whose expressions were female-predominant in hepatocytes. Moreover, liver expression of male-predominant genes such as ,, , and were reduced in male mice with hyperprolactinemia. The serum levels of conventional regulators of hepatic gene expressions, growth hormone, and testosterone were not affected by hyperprolactinemia. We demonstrated that prolactin upregulated female-predominant genes in female mice and downregulated male-predominant genes in male mice. We conjecture that higher concentration of prolactin would alter steroid and xenobiotic metabolisms by modulating hepatic gene expressions during pregnancy and lactation.

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Section: Resultsmentioning

confidence: 90%

Prolactin Upregulates Female-PredominantP450Gene Expressions and Downregulates Male-Predominant Gene Expressions in Mouse Liver

et al. 2017

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“…The quantitative evaluation was performed with aqman7500 system software (Applied Biosystems, Foster, MA, USA). Expression of genes was normalized to that of 18S rRNA .…”

Section: Methodsmentioning

confidence: 99%

Immunoprotective Effect of Chitosan Particles on Hymenolepis nana – Infected Mice

et al. 2017

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Hymenolepis nana is the most commonly known intestinal cestode infecting mainly human. This study aimed to investigate the potential effect of chitosan particles (CSP) to enhance the immune system against H. nana infection. Determination of worm burden, egg output, histopathological changes, oxidative stress markers (lipid peroxidation and reduced glutathione), goblet (GCs) and mucosal mast cells (MMCs) counts in intestinal ileum was performed. In addition, levels of intestinal mRNA expression of interleukin (IL)-4, IL-9, stem cell factor (SCF), type I and II interferons (IFN)-α/ γ, tumour necrosis factor (TNF)-α, mucin 2 (MUC2) and inducible nitric oxide synthase (iNOs) were investigated using real-time PCR. The results indicated induced reductions in adult worm and egg counts in infected mice after CSP treatment. This was associated with improvement in tissue morphometric measurements and oxidative stress which were altered after infection. Expression levels of iNOs, IFN-α, IFN-γ, TNF-α and IL-9 were decreased by CSP. Conversely, expression levels of MUC2, IL-4 and SCF increased compared to infected untreated group. In addition, GCs and MMCs counts were normalized by CSP. In conclusion, this study could indicate the immunoprotective effect of CSP against H. nana infection. This was characterized with Th2 anti-inflammatory responses.

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“…Quality and integrity of RNA were determined using the Agilent RNA 6000 Nano Kit on the Agilent 2100 Bioanalyzer (Agilent Technologies). RNA was quantified by measuring A 260nm on the ND-1000 spectrophotometer (NanoDrop Technologies) (Delic et al 2010). All RNA samples were treated with DNase (Applied Biosystems, Darmstadt, Germany) for at least 1 h and were then converted into cDNA using the reverse transcription kit following the manufacturer's protocol (Qiagen, Hilden, Germany).…”

Section: Quantitative Pcrmentioning

confidence: 99%

Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice

2015

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Malaria is still one of the most common infectious diseases and leads to various public health problems worldwide. Medicinal plants are promising sources for identifying novel agents with potential antimalarial activity. This study aimed to investigate the antimalarial and the antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice. Mice were divided into five groups. The first group served as a vehicle control; the second, third, fourth, and fifth groups were infected with 1 × 10(6) P. chabaudi-parasitized erythrocytes. Mice of the last three groups were gavaged with 100 μl of I. oblongifolia leave extract (IOLE) at a dose of 100, 200, and 300 mg IOLE/kg, respectively, once daily for 7 days. IOLE was significantly able to lower the percentage of parasitemia. The most effective dose was the 100 mg IOLE/kg, which could reduce the parasitemia from about 38 to 12 %. The infection induced spleen injury. This was evidenced by disorganization of spleen white and red pulps, appearance of hemozoin granules and parasitized erythrocytes. These changes in spleen led to the increased histological score. Also, the infection increased the spleen oxidative damage where the levels of nitrite/nitrate, malondialdehyde, and catalase were significantly altered. All these infection-induced parameters were significantly improved during IOLE treatment. In addition, the mRNA expression of inflammatory cytokines interleukin-1beta, interleukin-6, and tumor necrosis factor-alpha were upregulated after infection with P. chabaudi, whereas IOLE significantly reduced the expression of these genes. Our results indicate that I. oblongifolia leaves extract exhibits a significant antimalarial and antioxidant effects, and protects host spleen tissue from injuries induced by P. chabaudi.

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Testosterone-induced permanent changes of hepatic gene expression in female mice sustained during Plasmodium chabaudi malaria infection

Cited by 28 publications

References 56 publications

Prolactin Upregulates Female-PredominantP450Gene Expressions and Downregulates Male-Predominant Gene Expressions in Mouse Liver

Prolactin Upregulates Female-PredominantP450Gene Expressions and Downregulates Male-Predominant Gene Expressions in Mouse Liver

Immunoprotective Effect of Chitosan Particles on Hymenolepis nana – Infected Mice

Antimalarial and antioxidant activities of Indigofera oblongifolia on Plasmodium chabaudi-induced spleen tissue injury in mice

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