Testing the suitability of different high-performance liquid chromatographic methods to determine aflatoxin M1 in a soft fresh Italian cheese

Optimisation, validation and comparison of methods for aflatoxin M1 determination in cheese

“…Although some research (Cattaneo et␣al . 2011) has shown that better efficiency is achieved by enzymatic digestion, our results have shown high efficiency of extraction by blending with an organic solvent. It should be taken into account that the efficiency test was done using spiked samples.…”

Enzyme-assisted extraction for the HPLC determination of aflatoxin M1 in cheese

“…The suitability of the P-HCl method was also tested by participating in a collaborative study for AFM 1 determination in soft fresh cheese (Cattaneo et al, 2011). In this study, most of the participants used chloroform or dichloromethane to extract the toxin; however, the P-HCl method gave accurate results and the highest recovery values.…”

“…This method has been widely used and has been adopted as an official AOAC method (AOAC, 2005a). It showed a detection limit as low as 0.005 lg kg À1 (Pietri, Bertuzzi, Bertuzzi, & Piva, 1997) and sufficient repeatability, but AFM 1 recovery values were generally lower in comparison with milk, ranging from 51% to 88% (Cattaneo, Marinoni, Barzaghi, Cremonesi, & Monti, 2011;Dragacci et al, 1995;Govaris, Roussi, Koidis, & Botsoglou, 2001;Iha, Barbosa, Okada, & Trucksess, 2013;Oruc, Cibik, Yilmaz, & Kalkanli, 2006), probably because of the more complex clean-up step. Finally, this method is time consuming and uses a chlorinated solvent.…”

Inocuidad microbiológica de los alimentos para animales en Costa Rica

“…Por ejemplo, las aflatoxinas que son toxinas producidas por hongos filamentosos como producto de su metabolismo secundario son también contaminantes muy importantes de los alimentos para animales. Las aflatoxinas AFB 1 y AFG 1 son producidas por hongos ubicuos (naturales del ambiente) principalmente por Aspergillus parasiticus, A. flavus y A. nomius (Cattaneo et al 2011;Fallah et al, 2011). La producción de estas toxinas se ve favorecida en climas tropicales y subtropicales (Bbosa et al 2013;Britzi et al 2013) problemas tanto a humanos como a los animales.…”