2015
DOI: 10.1088/1367-2630/17/8/083049
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Testing the differential adhesion hypothesis across the epithelial−mesenchymal transition

Abstract: We analyze the mechanical properties of three epithelial/mesenchymal cell lines (MCF-10A, MDA-MB-231, MDA-MB-436) that exhibit a shift in E-, N-and P-cadherin levels characteristic of an epithelial−mesenchymal transition associated with processes such as metastasis, to quantify the role of cell cohesion in cell sorting and compartmentalization. We develop a unique set of methods to measure cell-cell adhesiveness, cell stiffness and cell shapes, and compare the results to predictions from cell sorting in mixtur… Show more

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Cited by 97 publications
(127 citation statements)
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References 68 publications
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“…We designed the setup in a minimalistic approach to change as few parameters as possible to keep the systems comparable. In this regard, we used the same lasers, optical fibers, and reservoirs as in previous studies concerning cancer research [4,8,39,59,60], various research questions of single cell rheology [38,40,41,61,62], and the principle of the Optical Stretcher as an optical rheometer for living cells [36,37,50]. The microfluidic surroundings have been adopted as far as possible, keeping the channel dimensions and path lengths, as well as flow velocities and pressure in the same order of magnitude.…”
Section: Resultsmentioning
confidence: 99%
“…We designed the setup in a minimalistic approach to change as few parameters as possible to keep the systems comparable. In this regard, we used the same lasers, optical fibers, and reservoirs as in previous studies concerning cancer research [4,8,39,59,60], various research questions of single cell rheology [38,40,41,61,62], and the principle of the Optical Stretcher as an optical rheometer for living cells [36,37,50]. The microfluidic surroundings have been adopted as far as possible, keeping the channel dimensions and path lengths, as well as flow velocities and pressure in the same order of magnitude.…”
Section: Resultsmentioning
confidence: 99%
“…In the presumptive enveloping layer on the zebrafish embryonic surface, for example, cell divisions that are oriented in-plane versus out-of-plane contribute to variations in epithelial cell shape that are widely distributed and approximate a log-normal distribution Box 1. Caging and disorder, glassiness and jamming Appreciable experimental evidence now supports the idea that a confluent cellular collective can exhibit both a jammed, solid-like phase (left panel) and an unjammed, fluid-like phase (right panel) along with characteristic changes in cell shape, and underlying theoretical considerations have become well developed Bi et al, 2015Bi et al, , 2016Garcia et al, 2015;Henkes et al, 2011;Nnetu et al, 2013Nnetu et al, , 2012Park et al, 2015b;Pawlizak et al, 2015;Tambe et al, 2011;Trepat et al, 2009). In the familiar fluid-to-solid transition of liquid water freezing to solid ice, there is a spontaneous molecular ordering from the amorphous disorder that typifies a fluid to the long-range order that typifies a crystalline solid, whereas in the jamming transition of particulate matter no such spontaneous structural ordering occurs; disorder persists in fluid-like and solid-like phases alike, the latter of which is referred to as a glassy solid Garrahan, 2011).…”
Section: Cell Shape and Cell Proliferationmentioning
confidence: 86%
“…On the one hand, most investigators now concur that the cellular collective tends to evolve toward an amorphous solid-like glassy phase Garcia et al, 2015;Garrahan, 2011;Nnetu et al, 2013Nnetu et al, , 2012Pawlizak et al, 2015;Tambe et al, 2011). But on the other hand, the mechanism or mechanisms through which this solid-like glassy phase comes about remains a matter of some dispute.…”
Section: Cell Shape Cell Jamming and The Vertex Modelmentioning
confidence: 99%
“…We show that the ability of cells to independently regulate adhesivity and tension, together with neighbor-based interaction rules, lets them support strikingly unusual interfaces. In particular, we show that mechanical-and fluctuation-based measurements of the effective surface tension of a cellular aggregate yield different results, leading to mechanically soft interfaces that are nevertheless extremely sharp.The process of compartmentalizing different cell populations, and maintaining those boundaries, is of vital importance in processes ranging from early embryonic development to tumor metastasis [1,2]. A common paradigm, the differential adhesion hypothesis, treats each cell population as an immiscible fluid and suggests that cell sorting and compartmentalization are driven by an effective surface tension [3], which is in turn governed by a competition between the repulsive and adhesive interactions between cells.…”
mentioning
confidence: 99%