Optical stretching in continuous flows

Morawetz, Erik; Stange, Roland; Kießling, T.; Schnauß, Jörg; Käs, Josef A.

doi:10.1088/2057-1739/aa6eb1

Cited by 10 publications

(8 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This particular type of cell disorder leads to larger tensions between adhered cells, which in turn gives rise to a percolating cluster of rigid cells responsible for the increase in the tissue rigidity. This result sheds light on the dynamics of cancer propagation, for cancer cells are usually softer than healthy ones [48][49][50]. Here, we show that the opposite behavior is observed when the disorder is on the substrate (position dependent).…”

Section: B)supporting

confidence: 53%

Substrate disorder promotes cell motility in confluent tissues

Pinto¹,

Gama²,

Araújo³

2021

Preprint

View full text Add to dashboard Cite

Section: B)supporting

confidence: 53%

Substrate disorder promotes cell motility in confluent tissues

Pinto¹,

Gama²,

Araújo³

2021

Preprint

View full text Add to dashboard Cite

“…With the two-beam optical stretcher single cells in suspension were initially trapped with a laser power of 100 mW using two facing and slightly divergent laser beams [9,11,20,23,32,33]. The cell suspension was given into a microfluidic system to channel a single cell into the optical trap.…”

Section: Optical Stretchermentioning

confidence: 99%

Changing cell mechanics—a precondition for malignant transformation of oral squamous carcinoma cells

Meinhövel

Stange

Schnauß

et al. 2018

Converg. Sci. Phys. Oncol.

Self Cite

View full text Add to dashboard Cite

Oral squamous cell carcinomas (OSCC) are the 6 th most common cancer and the diagnosis is often belated for a curative treatment. The reliable and early differentiation between healthy and diseased cells is the main aim of this study in order to improve the quality of the treatment and to understand tumour pathogenesis.Here, the optical stretcher is used to analyse mechanical properties of cells and their potential to serve as a marker for malignancy. Stretching experiments revealed for the first time that cells of primary OSCCs were deformed by 2.9 % rendering them softer than cells of healthy mucosa which were deformed only by 1.9 %. Furthermore, the relaxation behaviour of the cells revealed that these malignant cells exhibit a faster contraction than their benign counterparts. This suggests that deformability as well as relaxation behaviour can be used as distinct parameters to evaluate emerging differences between these benign and malignant cells. Since many studies in cancer research are performed with cancer cell lines rather than primary cells, we have compared the deformability and relaxation of both types, showing that long time culturing leads to softening of cells. The higher degree of deformability and relaxation behaviour can enable cancer cells to traverse tissue emphasizing that changes in cell architecture may be a potential precondition for malignant transformation. Respecting the fact that even short culture times have an essential effect on the significance of the results, the use of primary cells for further research is recommended. The distinction between malignant and benign cells would enable an early confirmation of cancer diagnoses by testing cell samples of suspect oral lesions.

show abstract

“…Recent experimental evidence [15][16][17][18] have revealed that tumor cells exhibit a broad distribution of various biomechanical properties. These include intra-tumor heterogeneity in cell stiffnesses [17,[19][20][21][22], stresses, and cell-cell interactions [21,23].…”

mentioning

confidence: 99%

“…However, there is no consensus on how these heterogeneities affect the mechanical behavior at the tissue level. For example, while biophysical techniques [24] such as AFM [22] and optical stretcher [18] show that individual cancer cells are softer than healthy cells, there is an apparent paradox with measurements at the tissue level, which show that tumors are more rigid than healthy tissues [25,26].…”

mentioning

confidence: 99%

Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

Das

2019

Phys. Rev. Lett.

View full text Add to dashboard Cite

We study the influence of cell-level mechanical heterogeneity in epithelial tissues using a vertex-based model. Heterogeneity in single cell stiffness is introduced as a quenched random variable in the preferred shape index(p 0 ) for each cell. We uncovered a crossover scaling for the tissue shear modulus, suggesting that tissue collective rigidity is controlled by a single parameter f r , which accounts for the fraction of rigid cells. Interestingly, the rigidity onset occurs at f r = 0.21, far below the contact percolation threshold of rigid cells. Due to the separation of rigidity and contact percolations, heterogeneity can enhance tissue rigidity and gives rise to an intermediate solid state. The influence of heterogeneity on tumor invasion dynamics is also investigated. There is an overall impedance of invasion as the tissue becomes more rigid. Invasion can also occur in the intermediate heterogeneous solid state and is characterized by significant spatial-temporal intermittency.

show abstract

Optical stretching in continuous flows

Cited by 10 publications

References 60 publications

Substrate disorder promotes cell motility in confluent tissues

Substrate disorder promotes cell motility in confluent tissues

Changing cell mechanics—a precondition for malignant transformation of oral squamous carcinoma cells

Mechanical Heterogeneity in Tissues Promotes Rigidity and Controls Cellular Invasion

Contact Info

Product

Resources

About