“…The discordance in the molecular results may lead one to conclude that all these samples are conspecific. However, our results do not necessarily show the species complex behaving as if they were a single species and the morphological differences mentioned in Slowik and Sikes (2013) are certainly within the amount currently accepted for species delineation in other members of Pardosa and lycosid spiders (Dondale and Redner 1990;Dondale 1999;Correa-Ramirez et al 2010;Slowik and Sikes 2013).…”
Section: Discussioncontrasting
confidence: 64%
“…Before our analyses only one phylogenetic study addressed species limits in the genus Pardosa. The study of Pardosa sierra Banks, 1898 (Correa-Ramirez et al 2010) used morphology with genetic-distance comparisons to delimit species. They found morphologic species were supported using DNA barcode identification.…”
Section: Discussionmentioning
confidence: 99%
“…3-4). If we had restricted our sampling to the strategy used by Correa-Ramirez et al (2010), in all likelihood, we would have falsely concluded that most, or possibly all, of the species were monophyletic. This should be considered justification for showing caution in interpretation of results from studies using small sample sizes and limited molecular data.…”
Section: Discussionmentioning
confidence: 99%
“…Several population genetic studies have been conducted on species groups found in Europe and Asia (Muster and Berendonk 2006; Chang et al 2007; Muster et al 2009) but these have largely ignored taxonomic issues and have focused more on gene flow and evolution of morphological variation. To date only one pardosine taxonomic work used both morphological and molecular methods (Correa-Ramirez et al 2010) in making species delimitations. However, their sample sizes were small, with only two to three specimens sequenced per species and they used only a short section of mtDNA.…”
Relationships within the Pardosa groenlandica species complex (sensu Slowik and Sikes 2013) were analysed to test two competing hypotheses – the species complex consists of either four or seven species. We conducted a partitioned Bayesian analyses of the mitochondrial gene COI and the nuclear genes ITS1, 5.8S, and ITS2. These genes provided a dataset composed of 1874 nucleotides each from 144 specimens. Additional analyses included application of the DNA barcoding protocol and a phylogeographic study of a subset of specimens. Analyses found no support for either hypothesis and only one species was found monophyletic in a subset of analyses. Mitochondrial DNA yielded clades discordant with geography. Species in the P. groenlandica species complex show various amounts of genetic support, with a general lack of agreement between genetics and morphology for species boundaries.
“…The discordance in the molecular results may lead one to conclude that all these samples are conspecific. However, our results do not necessarily show the species complex behaving as if they were a single species and the morphological differences mentioned in Slowik and Sikes (2013) are certainly within the amount currently accepted for species delineation in other members of Pardosa and lycosid spiders (Dondale and Redner 1990;Dondale 1999;Correa-Ramirez et al 2010;Slowik and Sikes 2013).…”
Section: Discussioncontrasting
confidence: 64%
“…Before our analyses only one phylogenetic study addressed species limits in the genus Pardosa. The study of Pardosa sierra Banks, 1898 (Correa-Ramirez et al 2010) used morphology with genetic-distance comparisons to delimit species. They found morphologic species were supported using DNA barcode identification.…”
Section: Discussionmentioning
confidence: 99%
“…3-4). If we had restricted our sampling to the strategy used by Correa-Ramirez et al (2010), in all likelihood, we would have falsely concluded that most, or possibly all, of the species were monophyletic. This should be considered justification for showing caution in interpretation of results from studies using small sample sizes and limited molecular data.…”
Section: Discussionmentioning
confidence: 99%
“…Several population genetic studies have been conducted on species groups found in Europe and Asia (Muster and Berendonk 2006; Chang et al 2007; Muster et al 2009) but these have largely ignored taxonomic issues and have focused more on gene flow and evolution of morphological variation. To date only one pardosine taxonomic work used both morphological and molecular methods (Correa-Ramirez et al 2010) in making species delimitations. However, their sample sizes were small, with only two to three specimens sequenced per species and they used only a short section of mtDNA.…”
Relationships within the Pardosa groenlandica species complex (sensu Slowik and Sikes 2013) were analysed to test two competing hypotheses – the species complex consists of either four or seven species. We conducted a partitioned Bayesian analyses of the mitochondrial gene COI and the nuclear genes ITS1, 5.8S, and ITS2. These genes provided a dataset composed of 1874 nucleotides each from 144 specimens. Additional analyses included application of the DNA barcoding protocol and a phylogeographic study of a subset of specimens. Analyses found no support for either hypothesis and only one species was found monophyletic in a subset of analyses. Mitochondrial DNA yielded clades discordant with geography. Species in the P. groenlandica species complex show various amounts of genetic support, with a general lack of agreement between genetics and morphology for species boundaries.
“…in [ 44 ]). Moreover, disputed instances of synonymy may be resolved [ 45 ]. Not least, a considerable advantage of barcoding is the possibility to identify juvenile specimens [ 3 , 46 – 49 ].…”
As part of the German Barcode of Life campaign, over 3500 arachnid specimens have been collected and analyzed: ca. 3300 Araneae and 200 Opiliones, belonging to almost 600 species (median: 4 individuals/species). This covers about 60% of the spider fauna and more than 70% of the harvestmen fauna recorded for Germany. The overwhelming majority of species could be readily identified through DNA barcoding: median distances between closest species lay around 9% in spiders and 13% in harvestmen, while in 95% of the cases, intraspecific distances were below 2.5% and 8% respectively, with intraspecific medians at 0.3% and 0.2%. However, almost 20 spider species, most notably in the family Lycosidae, could not be separated through DNA barcoding (although many of them present discrete morphological differences). Conspicuously high interspecific distances were found in even more cases, hinting at cryptic species in some instances. A new program is presented: DiStats calculates the statistics needed to meet DNA barcode release criteria. Furthermore, new generic COI primers useful for a wide range of taxa (also other than arachnids) are introduced.
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