Testing Somaclonal Variation Using RAPDs in Quercus suber L. Somatic Embryos

Gallego, Francisco Javier; Martinez, I.; Celestino, C.; Toribio, M.

doi:10.1086/297468

Cited by 36 publications

(18 citation statements)

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“…These results support the hypothesis that chimeric embryos were not present in the last stages of culture. On the other hand, although the cork oak recurrent system is genetically quite stable and hence the risk of somaclonal variation is low (Gallego et al 1997;Hornero et al 2001), the specific culture conditions used to transform cells and to select transgenic embryos may induce changes that should be monitored.…”

Section: Resultsmentioning

confidence: 99%

Genetic transformation of selected mature cork oak (Quercus suber L.) trees

et al. 2004

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A transformation system for selected mature cork oak (Quercus suber L.) trees using Agrobacterium tumefaciens has been established. Embryos obtained from recurrent proliferating embryogenic masses were inoculated with A. tumefaciens strains EHA105, LBA4404 or AGL1 harbouring the plasmid pBINUbiGUSint [carrying the neomycin phosphotransferase II (nptII) and beta-glucuronidase (uidA) genes]. The highest transformation efficiency (4%) was obtained when freshly isolated explants were inoculated with A. tumefaciens strain AGL1. Evidence of stable transgene integration was obtained by PCR for the nptII and uidA genes, Southern blotting and expression of the uidA gene. The transgenic embryos were germinated and successfully transferred to soil.

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Section: Resultsmentioning

confidence: 99%

Genetic transformation of selected mature cork oak (Quercus suber L.) trees

et al. 2004

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“…In micropropagated Q. serrate no aberrations in the banding patterns were detected by RAPD markers . Also, RAPDS were used to assess putative occurrence somaclonal variation in Q. suber embryogenic lines, but no molecular changes were found (Gallego et al, 1997, Sanchez et al, 2003. More recently, RAPDS were also used to evaluate genetic instability of somatic embryos of Q. suber obtained by the above described protocols ( Figure 6; Fernandes et al, 2011).…”

Section: Tissuementioning

confidence: 99%

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Santos¹

2012

Current Frontiers in Cryopreservation

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“…Aunque hay diversos estudios que señalan la estabilidad genética de embriones somá-ticos y de las plantas por ellos generadas (Heinze y Schmidt, 1995;Gallego et al, 1997;Engelborghs et al, 1998), otros trabajos indican la aparición de variaciones entre embriones somáticos (Vendrame et al 1999;Hornero et al, 2001). Se da el caso de encontrar variación entre embriones somáticos, pero no entre las plantas obtenidas de su germinación (Wilhelm et al, 2005).…”

Section: Fidelidad Clonalunclassified

Somatic embryogenesis as a main tool in forest biotechnology

Celestino¹,

Hernández²,

Carneros³

et al. 2005

For. syst.

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ResumenPara dar sentido agronómico a diferentes biotecnologías de aplicación, esto es, para poder transferir las posibilidades que ofrecen a la actividad operativa, es necesario disponer de una técnica fiable de regeneración de plantas. El cultivo de tejidos de genotipos valiosos, de meristemos libres de virus, de células transformadas, etc., no sirve de nada desde un punto de vista aplicado si de ellos no es posible conseguir la multiplicación en masa de dichos genotipos selectos, obtener plantas saneadas o lograr plantas transgénicas, en condiciones económicamente viables. La embriogénesis somática está ampliamente considerada como la mejor vía de regeneración que utiliza las técnicas de cultivo de tejidos vegetales en biotecnología forestal. Los avances actuales en biotecnología-genómica están teniendo un gran impacto en el uso de material selecto en muchos programas de mejora. La embriogénesis somática está contribuyendo a la producción de plantas transgénicas y a la consecución de la silvicultura clonal de alta productividad, con calidad de madera mejorada y menor impacto de enfermedades, en plantaciones forestales. Este artículo revisa la situación actual de la embriogénesis somática aplicada a especies forestales.Palabras clave: Regeneración de plantas, cultivo in vitro de tejidos vegetales. Summary Somatic embryogenesis as a main tool in forest biotechnologyTo give agronomic value, that is, to be able to transfer the benefits of different biotechnologies of application to operational purposes, a reliable plant regeneration technique is needed. Tissue culture of valuable genotypes, of virus-free meristems, of transformed cells, etc., is not worth if we are not able to mass propagating clonal plants, to obtain healthy plants or to achieve transgenic plants. Somatic embryogenesis is widely assumed to be the best tissue culture-based way of regenerating plants in forest biotechnology. Recent advances in biotechnology-genomics are having an important effect on the deployment of selected genetic plant materials in many breeding programs. Somatic embryogenesis is contributing to the release of transformed plants and to the implementation of clonal forestry with improved productivity, enhanced wood quality and reduced diseases in forest plantations. This article review the current status of somatic embryogenesis applied to forest species.

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Testing Somaclonal Variation Using RAPDs in Quercus suber L. Somatic Embryos

Cited by 36 publications

References 0 publications

Genetic transformation of selected mature cork oak (Quercus suber L.) trees

Genetic transformation of selected mature cork oak (Quercus suber L.) trees

Somatic Embryogenesis and Cryopreservation in Forest Species: The Cork Oak Case Study

Somatic embryogenesis as a main tool in forest biotechnology

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