Genetic transformation of selected mature cork oak (Quercus suber L.) trees

Álvarez, Rafael Jiménez-Camino; Alonso, P. Vega; Cortizo, Millán; Celestino, C.; Hernández, Inmaculada; Toribio, M.; Ordás, Ricardo J.

doi:10.1007/s00299-004-0810-2

Cited by 38 publications

(25 citation statements)

References 32 publications

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“…Transformation systems of poplar species (Fillatti et al 1987;Leple et al 1992) have been available for 20 years and the first studies on EM of transformed poplars have been published (Kaldorf et al 2002). With the more recent transformation of larch (Gleeson et al 2005) and birch (Vauramo et al 2006), but also of episodic growing trees like pines (Tang et al 2001) or oak (Alvarez et al 2004), the number of transformable trees is increasing. For the model tree oak, embryogenesis from tissues of mature oak trees (Quercus suber) is possible (Valladares et al 2006;Lopes et al 2006).…”

Section: Discussionmentioning

confidence: 98%

Why and How Using Micropropagated Trees rather than Germinations for Controlled Synthesis of Ectomycorrhizal Associations?

Herrmann

Buscot

2008

Mycorrhiza

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Section: Discussionmentioning

confidence: 98%

Why and How Using Micropropagated Trees rather than Germinations for Controlled Synthesis of Ectomycorrhizal Associations?

Herrmann

Buscot

2008

Mycorrhiza

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“…Various studies show that members of the Fagaceae can be transformed using A. tumefaciens by manipulating several factors (Á lvarez et al 2004;Corredoira et al 2004Corredoira et al , 2006Polin et al 2005;Seabra and Pais 1998). In previous experiments (Á lvarez et al 2004), we observed that cork oak somatic embryos were susceptible to AGL1, a disarmed succinamopine-nopaline strain of A. tumefaciens (Lazo et al 1991).…”

Section: Introductionmentioning

confidence: 91%

Improved genetic transformation protocol for cork oak (Quercus suber L.)

Álvarez

Ordás

2007

Plant Cell Tiss Organ Cult

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An optimized protocol for Agrobacterium tumefaciens-mediated transformation of mature Quercus suber L. embryogenic masses is reported. In this work several variables were tested. Plant genotype, explant type and time elapsed between the last subculture and inoculation, i.e. the explant preculture period, were found to be very important. Interaction between inoculum density and cocultivation period influenced the transformation efficiency as well. A transformation efficiency (i.e. percentage of the inoculated explants that yielded independent transgenic embryogenic lines) of up to 43% was obtained, greatly improving the previously described method for plant transformation of adult-selected cork oak. It was also shown that this protocol could be applied to various genotypes.

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“…Genetic transformation protocols for cork oak embryogenic lines induced from both mature (Alvarez et al 2004) and juvenile (Sánchez et al 2005) materials through coculture with different strains of Agrobacterium tumefaciens harboring plasmids containing marker genes have been defined. An optimized protocol for Agrobacterium-mediated transformation of mature embryogenic masses of this species, in which variables such as the genotype, explant type, and the preculture period were studied, has also been reported (Alvarez and Ordás 2007).…”

Section: Genetic Transformationmentioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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The genus Quercus, which belongs to the family Fagaceae, is native to the northern hemisphere and includes deciduous and evergreen species. The trees of the different species are very important from both economic and ecological perspectives. Application of new technological approaches (which span the fields of plant developmental biology, genetic transformation, conservation of elite germplasm and discovery of genes associated with complex multigenic traits) to these long-rotation hardwoods may be of interest for accelerating tree improvement programs. This review provides a summary of the advances made in the application of biotechnological tools to specific oak species. Significant progress has been made in the area of clonal propagation via organogenesis and somatic embryogenesis (SE). Standardized procedures have been developed for micropropagating the most important European (Q. robur, Q. petarea, Q. suber) and American (Q. alba, Q. bicolor, Q. rubra) oaks by axillary shoot growth. Although regenerated plantlets are grown in experimental trials, large-scale propagation of oak species has not been carried out. The induction of SE in oaks from juvenile explants is generally not problematic, although the use of explants other than zygotic embryos is much less efficient. During the last decade, enormous advances have been made in inducing SE from selected adult trees, mainly specimens of pedunculate oak (Q. robur) and cork oak (Q. suber). Advances in the understanding of the maturation and germination steps are required for better use of embryogenic process in clonal forestry. Quercus species are late-maturing and late-flowering, exhibit irregular seed set, and produce seeds that are recalcitrant to storage by conventional procedures. Vitrification-based cryopreservation techniques were used successfully in somatic embryos of pedunculate oak and cork oak, and an applied genbank of cork oak selected genotypes is now under development. The feasibility of genetic transformation of pedunculate oak and cork oak somatic embryos by means of co-culture techniques with several strains of Agrobacterium tumefaciens has also been demonstrated. To date, most research on the genomics of Quercus species has concerned population genetics. Approaches using functional genomics to examine the molecular and cellular mechanisms that control organogenesis and or somatic embryogenesis are still scarce, and efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future, as this would help improve the practical application of clonal forestry in recalcitrant species such as oaks.

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Genetic transformation of selected mature cork oak (Quercus suber L.) trees

Cited by 38 publications

References 32 publications

Why and How Using Micropropagated Trees rather than Germinations for Controlled Synthesis of Ectomycorrhizal Associations?

Why and How Using Micropropagated Trees rather than Germinations for Controlled Synthesis of Ectomycorrhizal Associations?

Improved genetic transformation protocol for cork oak (Quercus suber L.)

Application of biotechnological tools to Quercus improvement

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