Improved genetic transformation protocol for cork oak (Quercus suber L.)

Álvarez, Rafael Jiménez-Camino; Ordás, Ricardo J.

doi:10.1007/s11240-007-9276-6

Cited by 22 publications

(18 citation statements)

References 36 publications

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“…The transformation frequencies obtained in the present study are sufficient for transformation of recalcitrant species (Matsunaga et al 2012). Within the genus Quercus, Q. suber is the only other species in which genetic transformation on semi-solid medium has been investigated (Álvarez and Ordás 2007). In the present study, the transformation efficiencies achieved with TIS were similar to those reported for transformed embryogenic lines of cork oak, although transformation of some lines of the latter species was not successful (Álvarez and Ordás 2007).…”

Section: Discussionsupporting

confidence: 75%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

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As the limiting factor for recovery of plants from oak embryogenic lines is the low embryo conversion rate, axillary shoot lines were established from transformed germinated embryos.Transformed embryos and shoots were cultured in medium with or without kanamycin and the responses to several morphogenetic processes (recovery after cryopreservation, germination, shoot proliferation, and rooting) were evaluated.2

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Section: Discussionsupporting

confidence: 75%

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Mallón

Viéitez

Vidal

2013

Plant Cell Tiss Organ Cult

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“…Mukhopadhyay et al (1992) reported that, in oilseed Brassica campestris, hypocotyl explants were more amenable to transformation when compared with cotyledons. Similarly, differences in transformation with regard to explant type have been reported in other plant species such as tomato (Ö ktem et al 1999), Vigna radiata (Saima and Bushra 2004), oak (Rubén and Ricardo 2007) and Capsicum (Sanatombi and Sharma 2008). The variation in the transformation efficiency of different explants may be related to differences in the regenerative potential of the explants, which in turn is regulated by levels of endogenous hormones.…”

Section: Explant Typementioning

confidence: 64%

Agrobacterium-mediated genetic transformation of safflower (Carthamus tinctorius L.)

Shilpa

Kumar

Sujatha

2010

Plant Cell Tiss Organ Cult

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Reproducible and highly efficient protocols for shoot regeneration and genetic transformation mediated by Agrobacterium have been established for safflower (Carthamus tinctorius L.). Agrobacterium tumefaciens strain LBA 4404 with gus reporter gene and hygromycin (hpt gene) as plant selection marker was used as the plant transformation vector. Genetic transformation experiments were carried out to evaluate the efficacy of various parameters such as genotype, seedling age, co-cultivation period, bacterial titer, enzymatic pre-treatment of target tissues, use of compounds that induce vir-gene enhancer, acetosyringone (AS), explant type and explant injury to enhance transformation efficiency. Transformation frequency was high when root and hypocotyl explants of 8-day-old seedlings of safflower cv. HUS-305 were co-cultivated with bacterial cell density of 0.5 OD 600 during a period of 2 days followed by selection regime of 10-15-15 mg/l hygromycin. The frequency of rooting of the primary transformants was low (18.0%) when compared with the regenerated shoots (70.0%), and seven shoots survived on transfer to soil. The putative transformants were confirmed by b-glucuronidase (GUS) histochemical assay, polymerase chain reaction (PCR), reverse-transcriptase PCR (RT-PCR) and Southern blot analysis. With the optimized transformation protocol, putative transformed shoots were obtained with frequency of 51.0% within 8-10 weeks of culture initiation.

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“…An optimized protocol for Agrobacterium-mediated transformation of mature embryogenic masses of this species, in which variables such as the genotype, explant type, and the preculture period were studied, has also been reported (Alvarez and Ordás 2007). Genotype has a strong influence on the transformation frequency, as only 3 out of the 6 embryogenic lines tested were kanamycin-resistant after 4 months of culture in selective media.…”

Section: Genetic Transformationmentioning

confidence: 99%

Application of biotechnological tools to Quercus improvement

et al. 2011

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The genus Quercus, which belongs to the family Fagaceae, is native to the northern hemisphere and includes deciduous and evergreen species. The trees of the different species are very important from both economic and ecological perspectives. Application of new technological approaches (which span the fields of plant developmental biology, genetic transformation, conservation of elite germplasm and discovery of genes associated with complex multigenic traits) to these long-rotation hardwoods may be of interest for accelerating tree improvement programs. This review provides a summary of the advances made in the application of biotechnological tools to specific oak species. Significant progress has been made in the area of clonal propagation via organogenesis and somatic embryogenesis (SE). Standardized procedures have been developed for micropropagating the most important European (Q. robur, Q. petarea, Q. suber) and American (Q. alba, Q. bicolor, Q. rubra) oaks by axillary shoot growth. Although regenerated plantlets are grown in experimental trials, large-scale propagation of oak species has not been carried out. The induction of SE in oaks from juvenile explants is generally not problematic, although the use of explants other than zygotic embryos is much less efficient. During the last decade, enormous advances have been made in inducing SE from selected adult trees, mainly specimens of pedunculate oak (Q. robur) and cork oak (Q. suber). Advances in the understanding of the maturation and germination steps are required for better use of embryogenic process in clonal forestry. Quercus species are late-maturing and late-flowering, exhibit irregular seed set, and produce seeds that are recalcitrant to storage by conventional procedures. Vitrification-based cryopreservation techniques were used successfully in somatic embryos of pedunculate oak and cork oak, and an applied genbank of cork oak selected genotypes is now under development. The feasibility of genetic transformation of pedunculate oak and cork oak somatic embryos by means of co-culture techniques with several strains of Agrobacterium tumefaciens has also been demonstrated. To date, most research on the genomics of Quercus species has concerned population genetics. Approaches using functional genomics to examine the molecular and cellular mechanisms that control organogenesis and or somatic embryogenesis are still scarce, and efforts on the isolation and characterization of genes related to other specific traits should be intensified in the near future, as this would help improve the practical application of clonal forestry in recalcitrant species such as oaks.

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Improved genetic transformation protocol for cork oak (Quercus suber L.)

Cited by 22 publications

References 36 publications

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

High-efficiency Agrobacterium-mediated transformation in Quercus robur: selection by use of a temporary immersion system and assessment by quantitative PCR

Agrobacterium-mediated genetic transformation of safflower (Carthamus tinctorius L.)

Application of biotechnological tools to Quercus improvement

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