Testing for Anti-Human Transglutaminase Antibodies in Saliva Is Not Useful for Diagnosis of Celiac Disease

Baldas, Valentina; Tommasini, Alberto; Santon, Daniela; Not, Tarcisio; Gerarduzzi, Tania; Clarich, G; Sblattero, Daniele; Marzari, Roberto; Florian, Fiorella; Martellossi, Stefano; Ventura, Alessandro

doi:10.1373/clinchem.2003.023523

Cited by 14 publications

(10 citation statements)

References 17 publications

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“…In a previous study, 11 analysing the serum of CD patients at diagnosis and during the follow‐up on a GFD, we demonstrated that, for detection of CD‐related autoimmunity, a fluid‐phase RIA method is more sensitive than solid‐phase ELISA and IF EMA assays. Subsequently, using a similar fluid‐phase RIA format and in contrast with data obtained with a salivary ELISA immunoassay, 22 we found that it is possible to detect with high sensitivity, the tTG autoantibodies at the CD diagnosis also in human saliva 17 . In this study, we confirm and extend our previous results, demonstrating that it is possible to detect salivary tTG autoantibodies with high sensitivity also in coeliac patients on a GFD, when the autoantibody titres tend to decrease quite dramatically.…”

Section: Discussionmentioning

confidence: 99%

Radioimmunological detection of anti‐transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow‐up

Bonamico

Nenna²,

Luparia³

et al. 2008

Aliment Pharmacol Ther

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Section: Discussionmentioning

confidence: 99%

Radioimmunological detection of anti‐transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow‐up

Bonamico

Nenna²,

Luparia³

et al. 2008

Aliment Pharmacol Ther

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“…In particular, over the whole sample, 31 children have showed correspondence with the anti-tTG both in the serum and the salivary fluid, thus con- firming the hypothesis that the saliva can be a valuable help for diagnostic screening of such pathology (11). However, a previous study, conducted by Baldas, sayed that the saliva, because of its low sensitivity, couldn't be considered as a source of the typical auto-antibodies of the CD and for this reason such biological fluid couldn't be used for diagnostic purposes for the earlier interception of the disease (17). Before the discover of the salivary anti-tTG, several studies have been carried out in order to seek the specific salivary antibodies for the diagnosis of the CD; in particular anti-gliadin deaminated antibodies (AGA) and anti-endomysium antibodies (EMA) have been evaluated and analyzed (18,25).…”

Section: Discussionmentioning

confidence: 99%

The anti-transglutaminase auto-antibodies in children�s saliva with a suspect coeliac disease: clinical study

Condò

Costacurta²,

Docimo³

2013

ORL

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SUMMARYThe coeliac disease is an immune-mediated enteropathy triggered by an ingestion of gluten in genetically susceptible individuals. Like some other systemic diseases (Crohn's disease, Sjögren's syndrome) the celiac disease is able to alter the oral ecosystem and the composition of the saliva. Aim. The aim of this retrospective study has been to examine the incidence of coeliac disease (CD) in paediatric population and to search the presence of anti-transglutaminase auto-antibodies (anti-tTG) in saliva, comparing and quantifying the concentration regard to the serum values of the anti-tTG auto-antibodies, before and after six months from the beginning of the free gluten diet. Materials and Methods. 105 children (G 0 ), aged between 5 and 13 years, belonging to the Paediatric GastroenterologyEndoscopy Unit of PTV Hospital, University of Rome "Tor Vergata", have been examined for a diagnosis of suspected CD. Results. Of a total of 105 pediatric patients (G 0 ), only the 16.2% (G 1 ) has showed to be positive. About the evaluation of the anti-tTG auto-antibodies in the serum, obtained from the second blood sample (T 1 ), we can observe that 10 (G 2 ) out of 17 children (G 1 ) show positivity and for this reason they have been subjected to a sampling of intestinal villi to confirm the diagnosis of CD; in addition the 6.7% has been resulted positive at the first sampling of serum (T 0 ), but negative to the second one (T 1 ). The incidence of the CD has been resulted to be equal to 9.5%. About the evaluation of anti-tTG in the G1, we can observe that 58.8% of children are "definitely positive" to the salivary anti-tTG, while 11.8% appear to be weakly positive. About the correspondence of serum and salivary anti-tTG in Group G1, we can observe, that children positive to the anti-tTG in the serum have also the anti-tTG in the salivary fluid (sensibility 100%, specificity 71.4%). The results show that the anti-tTG salivary are present in children with CD, even though they have continued to follow the gluten free diet for 6 months. Conclusions. The presence of anti-tTG in the saliva may be considered, an additional and useful diagnostic dental marker for an initial, reproducible, non invasive, inexpensive and highly sensitive screening of CD having a predictive and precocious value compared to anti-tTG contained in the serum, as it has been already demonstrated.

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“…Baldas et al. demonstrated that salivary IgA transglutaminase antibody has low sensitivity and is therefore not particularly useful for diagnosing Crohn's disease. By contrast, several studies have demonstrated that salivary detection of IgA transglutaminase antibody is a sensitive, reproducible and reliable method for screening for Crohn's disease .…”

Section: Salivary Changes In Systemic Diseases With Possible Oral Mucmentioning

confidence: 99%

Salivary changes in oral mucosal diseases

Hassona¹,

Scully²

2015

Periodontology 2000

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Saliva is a unique biological fluid that can be easily collected and analyzed with low cost and low morbidity. Therefore, there is a growing attention for using salivary biomarkers in the diagnosis and monitoring of disease progress and response to treatment. Salivary changes have been described in relation to oral mucosal diseases. This article discusses the causes and consequences of salivary hypofunction and presents a review of the literature related to changes in salivary parameters in various oral mucosal diseases and in systemic diseases with possible oral mucosal involvement.

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Testing for Anti-Human Transglutaminase Antibodies in Saliva Is Not Useful for Diagnosis of Celiac Disease

Cited by 14 publications

References 17 publications

Radioimmunological detection of anti‐transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow‐up

Radioimmunological detection of anti‐transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow‐up

The anti-transglutaminase auto-antibodies in children�s saliva with a suspect coeliac disease: clinical study

Salivary changes in oral mucosal diseases

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