Radioimmunological detection of anti‐transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow‐up

Bonamico, Margherita; Nenna, Raffaella; Luparia, R.P.L.; Perricone, Carlo; Montuori, Monica; Lucantoni, Federica; Castronovo, Antonella; Mura, Silvia; Turchetti, A; Strappini, P. M.; Tiberti, Claudio

doi:10.1111/j.1365-2036.2008.03720.x

Cited by 25 publications

(27 citation statements)

References 32 publications

(38 reference statements)

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“…This result is similar to those seen in previous studies. [5][6][7]9,22 In contrast, Mohiti-Ardekani et al demonstrated that s-IgA levels in diabetic patients were higher than in non-diabetics. 1 Other studies have also found higher s-IgA levels in diabetic patients.…”

Section: Discussionmentioning

confidence: 99%

“…1,5 Other studies have also reported conflicting results with regards to s-IgA levels and their relationship to various diseases. 6,7 The current study aimed to compare s-IgA levels between healthy subjects and T2DM patients and determine the association between s-IgA levels and various oral and dental manifestations in diabetic patients. This relationship could help determine the prognosis of oral diseases in diabetic patients and serve as a guide for their dental care.…”

Section: Advances In Patient Care -Determination Of the Salivary Compmentioning

confidence: 99%

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Evaluation of Salivary Secretory Immunoglobulin A Levels in Diabetic Patients and Association with Oral and Dental Manifestations

Kakoei

Hosseini

Haghdoost

et al. 2015

SQUMJ

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abstract:Objectives: Oral and dental manifestations in diabetic patients can arise due to numerous factors, including elevated salivary secretory immunoglobulin A (s-IgA) levels. This study aimed to evaluate s-IgA concentrations in patients with type 2 diabetes mellitus (T2DM) and to investigate the association between s-IgA levels and oral and dental manifestations of T2DM. Methods: This cross-sectional descriptive study was carried out between October 2011 and September 2012 in Kerman, Iran, and included 260 subjects (128 patients with T2DM and 132 healthy controls). Unstimulated salivary samples were collected from all subjects and s-IgA levels were determined using the immunoturbidimetric method. The oral cavities and teeth of T2DM patients were evaluated for oral and dental manifestations. Results: Both diabetic and control subjects with higher concentrations of s-IgA had significantly higher numbers of decayed, missing or filled teeth (DMFT) and periodontal index (PDI) scores (P <0.050). s-IgA levels were significantly higher in subjects with oral candidiasis (P <0.050). Among diabetic patients, significantly higher s-IgA levels were concomitant with xerostomia and denture stomatitis (P ≤0.050). There were no significant differences between s-IgA concentrations and other oral or dental manifestations in either group. Conclusion: Individuals with a greater number of DMFT, a higher PDI score and oral candidiasis had significantly higher s-IgA levels. s-IgA levels were not significantly higher among diabetic patients in comparison to the control group. However, significantly higher s-IgA levels occurred with xerostomia and denture stomatitis in diabetic patients. In addition, s-IgA was significantly higher in patients with uncontrolled diabetes compared to those with controlled diabetes.

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Section: Discussionmentioning

confidence: 99%

Section: Advances In Patient Care -Determination Of the Salivary Compmentioning

confidence: 99%

Evaluation of Salivary Secretory Immunoglobulin A Levels in Diabetic Patients and Association with Oral and Dental Manifestations

Kakoei

Hosseini

Haghdoost

et al. 2015

SQUMJ

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“…Salivary biomarkers are used for screening purposes in epidemiological studies [13] and are being used to monitor and detect various diseases, such as breast cancer [14], lung cancer [15], celiac disease [16], and chronic renal failure [11]. With regard to oral health, three important salivary variables should be investigated: pH, flow velocity, and buffering capacity.…”

Section: Discussionmentioning

confidence: 99%

Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

Buche¹,

Gusso²,

Torres³

et al. 2017

Rev. Odonto Ciênc. (Online)

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OBJECTIVE: Human saliva has numerous components that are important for maintaining oral health. We investigated the association between iron levels in saliva in children and their caries experience. METHODS: We conducted a case-control study that included 186 healthy children, aged 11-14 years. The decayed-filled teeth/decayed-missing-filled teeth (DMFT) score was estimated according to the Brazilian Oral Health Project. The children were divided into two groups: caries-free group (DMFT=0, n=55) and caries experience group (DMFT≥1, n=131). Saliva was collected from each child using the spitting method and analyzed for stimulated saliva flow (SSF), pH, and iron levels. The SSF, pH, and salivary iron levels were analyzed for statistical assumptions of normality and homoscedasticity and compared between groups. All of the statistical tests were performed using R and SPSS software, with a level of significance of 0.05. RESULTS: No statistically significant differences in mean pH were found between groups (t=-0.0488, p=0.51). Children with caries experience had significantly lower salivary iron levels than caries-free children (w=5088, p<0.0001). The SSF values were significantly different between children with caries experience and children without caries (w=4198, p=0.03). CONCLUSION: Salivary iron levels were significantly lower in children with dental caries experience, suggesting that salivary iron plays a role in maintaining oral health.Key words: Saliva; Salivary iron; Caries Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças RESUMO OBJETIVO: Investigar a associação entre os níveis de ferro na saliva das crianças e sua experiência de cárie. MÉTODOS: Realizamos um estudo caso-controle envolvendo 186 crianças saudáveis de 11 a 14 anos. O número de dentes cariados, perdidos e obturados (CPO-D) foi estimado de acordo com o Projeto Saúde Bucal Brasil (SBBrasil) e as crianças foram divididas em dois grupos: grupo sem cárie (CPO-D=0; n=55) e experiência Grupo de cárie (CPO-D≥1; n=131). A saliva foi coletada de cada indivíduo usando o método de cuspir e analisada quanto ao fluxo estimado de saliva (SSF), pH e concentração de ferro. As variáveis SSF, pH e ferro salivar foram analisadas pelos pressupostos estatísticos de normalidade e homocedasticidade e comparados entre os grupos. Todos os testes foram realizados utilizando os programas estatísticos R (R Core Team, 2016) e SPSS (IBM, EUA), assumindo um nível de significância de 0,05. RESULTADOS: Não houve diferenças estatisticamente significativas entre os meios de pH entre os grupos (t=-0,0488; p=0,51). Em relação ao ferro salivar, as crianças com experiência em cárie apresentaram valores significativamente mais baixos de ferro salivar do que aqueles que não apresentavam caries (w=5088; p<0,0001). Além disso, os valores de SSF foram diferentes entre as crianças com experiência de cárie e aqueles sem cárie (w=4198; p=0,03). CONCLUSÃO: os níveis de ferro salivar foram estatisticamente inferiores em crianças com cárie den...

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“…The research on anti-tTG IgA is highly sensitive to CD diagnosis and for the follow up of patients under gluten-free diet. 19 …”

Section: Anti-tissue Transglutaminase (Anti-ttg Iga)mentioning

confidence: 99%

Diagnóstico de doença celíaca em adultos

Silva

Furlanetto

2010

Rev. Assoc. Med. Bras.

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Celiac disease (CD) is found in genetically predisposed individuals, and characterized by intolerance to gluten ingestion, contained in cereals such as barley, rye, wheat and malt. Clinical manifestations vary from asymptomatic patients to severe forms of malabsorption syndromes, which may involve multiple systems and increase the risk of some neoplasias. Diagnosis of CD often requires a high degree of suspicion. There is not a single test for the diagnosis, which is reached after a combination of clinical and laboratory data. The first step may be a serum test, such as the antibodies anti-tissue transglutaminase, or antiendomisio. If serum result is positive, duodenal biopsy is necessary for diagnostic confirmation. IgA deficiency, which occurs in 3% of patients with DC, may lead to false-negatives because serology is based on IgA antibodies. Another cause of false-negative tests is diet restriction of gluten; therefore diagnostic investigation must be carried out during a diet containing gluten. The screening for CD in asymptomatic individuals is not indicated.

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Radioimmunological detection of anti‐transglutaminase autoantibodies in human saliva: a useful test to monitor coeliac disease follow‐up

Abstract: SUMMARY BackgroundSerum radioimmunoassay (RIA) tissue transglutaminase autoantibodies (tTG-Abs) proved to be a sensitive test also during coeliac disease (CD) follow-up. We demonstrated that RIA tTG-Abs could be detected in human saliva.

Cited by 25 publications

References 32 publications

Evaluation of Salivary Secretory Immunoglobulin A Levels in Diabetic Patients and Association with Oral and Dental Manifestations

Evaluation of Salivary Secretory Immunoglobulin A Levels in Diabetic Patients and Association with Oral and Dental Manifestations

Associação entre a diminuição do nível de ferro salivar e a experiência de cárie em crianças

Diagnóstico de doença celíaca em adultos

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