We investigate the equation of state of hadronic and quark-gluon matter at finite temperature and baryon density considering the possible formation of mixed phase in high energy heavy ion collisions. The analysis is performed by requiring the Gibbs conditions on the global conservation of baryon number, electric charge and strangeness number. For hadronic phase, we study an extended relativistic mean-field theoretical model with the inclusion of ∆-isobar, hyperons and lightest pseudoscalar and vector mesons degrees of freedom. For the quark sector, we employ a MIT-Bag model with a Bag "constant" depending on the baryon chemical potential. In this context, the behavior of strangeness densities and strangeness-antistrangeness separation in the hadron-quark-gluon mixed phase are analyzed.