Testicular testosterone: Estradiol ratio in domestic cats and its relationship to spermatogenesis and epididymal sperm morphology

Muller, G.; Martino‐Andrade, Anderson Joel; Santos, A. S.; Reghelin, Angela L.; Garcia, Dorival; Sant'Ana, G.R.; Spercoski, Katherinne Maria; Meyer, Katlyn Barp; Torres, Sandra Maria de; Júnior, Valdemiro Amaro Silva; Morais, Rosana Nogueira de

doi:10.1016/j.theriogenology.2012.05.018

Cited by 24 publications

(19 citation statements)

References 60 publications

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“…The present study demonstrates evident alterations in testicular volume (partial and total) and sperm quality (concentration) in the cauda epididymidis (R3), revealing important differences between teratozoospermic and normozoospermic feline donors. In contrast to the present study, Müller et al 6. found no differences in testicular size or sperm production between the two donor groups.…”

Section: Discussioncontrasting

confidence: 99%

“…After this complex maturation process, which involves chemical and physical alterations from the caput and through the corpus epididymidis, the spermatozoa reach the cauda epididymidis where they will be stored before ejaculation. Under normal physiological conditions, the morphological sperm changes that take place during passage through the epididymal duct primarily involve modifications to the sperm head, with final adjustment of sperm dimension and shape 56…”

Section: Introductionmentioning

confidence: 99%

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Normozoospermic versus teratozoospermic domestic cats: differential testicular volume, sperm morphometry, and subpopulation structure during epididymal maturation

Gutiérrez-Reinoso

García-Herreros

2016

Asian J Androl

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Teratozoospermia (<40% morphologically normal spermatozoa/ejaculate) is a frequent phenomenon in feline species. This research was carried out to study the possible differences in testicular volume, differential sperm morphometric traits, and potential differences regarding the sperm subpopulational structure during epididymal sperm maturation in teratozoospermic feline donors. Epididymal sperm samples were collected from the caput (R1), corpus (R2), and cauda (R3) epididymidis in two donor groups (N: normozoospermic; T: teratozoospermic). Aliquots were assessed for concentration, viability, motility, and acrosomal integrity. Sperm morphometric descriptors from CASA-Morph analysis were analyzed by the Principal Component Analysis (PCA) and clustering analyses. Irrespective of the group analyzed, PCA revealed two Principal Components (PCs) for each epididymal region explaining more than the 93% of the variance. Surprisingly, the number of subpopulations remained constant in regions R1-R2-R3 irrespective of the donor group analyzed. However, the distribution of these subpopulations was found to be structurally different and strongly influenced by the epididymal region and the donor group. In conclusion, testicular morphometry and the sperm subpopulation structure were different in N and T donors. The alterations in subpopulations during epididymal maturation could be used as a potential clinical indicator of teratozoospermic individuals since an important influence of teratozoospermia on sperm subpopulation structure has been demonstrated.

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Section: Discussioncontrasting

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Normozoospermic versus teratozoospermic domestic cats: differential testicular volume, sperm morphometry, and subpopulation structure during epididymal maturation

Gutiérrez-Reinoso

García-Herreros

2016

Asian J Androl

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“…According to Muller et al (2012), for semen samples to be considered morphologically normal, it is necessary that they contain over 60% of normal cells. Thus, samples of all groups evaluated in this study had a percentage of normal cells above the minimum established, characterizing them as normospermic.…”

Section: Discussionmentioning

confidence: 99%

Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders

Lima

Silva

Cortez

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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We aimed to compare fresh sperm and sperm cooled to 4ºC that had been recovered from the epididymides of cats using powdered coconut water (ACP-117c) and Tris extenders. Sixty epididymides were divided into 6 groups: 10 fresh epididymides were recovered using Tris (T0h); 10 were kept at 4°C/2h and recovered using Tris (T2h); 10 were kept at 4°C/4h and recovered using Tris (T4h); 10 fresh were recovered using ACP-117c (A0h); 10 were kept at 4°C/2h and recovered using ACP-117c (A2h), and 10 were kept at 4°C/4h and recovered using ACP-117c (A4h). The testis-epididymis complexes (TEC) control were not cooled. The others were cooled at 4°C for 2 or 4h. The epididymis was separated and the sperm was recovered by the modified flotation method. Sperm kinetic parameters were evaluated by a computer-system analysis, and vigor, viability, concentration, membrane function and morphology of the sperm were assessed under a light microscope. The progressive motility with ACP-117c declined after 2h of cooling, but did not differ between fresh and 4h. The vigor and membrane function were higher in A4h than A0h. The vigor at T2h and T4h were decreased compared to T0h. T0h was higher than A0h for vigor and sperm membrane function. However, after 4h of cooling, ACP-117c maintained a higher percentage of living cells. Feline epididymal sperm quality can be maintained to the degree necessary for artificial breeding programs following cooling and ACP-117c may be successfully used to recover cat sperm that have been cooled for up to 4h.

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“…A total of 200 cells with abnormalities (one per sperm) were examined, and the results are reported as percentages (DIC microscopy, oil immersion, 1000×, Olympus BX61). Spermatozoa in which cytoplasmic droplets were the only alteration were considered normal (Axnér et al, 2004;Müller et al, 2012) because such droplets move during epididymal transit and might be lost during ejaculation (Axnér et al, 1999).…”

Section: Sperm Morphologymentioning

confidence: 99%

Evaluation of sperm head dimensions and chromatin integrity of epididymal sperm from domestic cats using the toluidine blue technique

Alves

Cancelli

Grassi

et al. 2018

Animal Reproduction Science

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When using assisted reproductive technologies, there is seldom an evaluation of DNA integrity during sperm analysis, which is an important variable for proper embryo development. The toluidine blue staining technique allows the simultaneous evaluation of sperm chromatin and sperm head dimensions. The objectives of this study were to evaluate the applicability of the toluidine blue staining method for analyzing DNA abnormalities in epididymal sperm (from the caput, corpus, and cauda) of cats and to investigate the correlations among DNA condensation, morphology, and sperm head dimensions. The DNA alteration indexes were obtained using the toluidine blue and acridine orange techniques, and comparisons of these indexes indicated there was a 65.4% (r = 0.654; P < 0.001) correlation. The sperm from the cauda had greater chromatin stability (97.9%) than the sperm from the epididymal head (92.1%; P = 0.0023). There, however, was no difference in chromatin stability between sperm obtained from the corpus and cauda regions, indicating that these sperm were already mature. The sperm head dimension was correlated with chromatin decondensation, and the sperm head size decreased as the sperm were transported through the three epididymal regions (P < 0.0001). In addition, the percentage of sperm that were deficient in chromatin condensation decreased as the sperm were transported through the epididymal caput, corpus and cauda (26.4, 15.7, and 3.4%, respectively; P < 0.0001). Thus, the sperm head size predicts the quality of chromatin condensation in sperm cells.

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Testicular testosterone: Estradiol ratio in domestic cats and its relationship to spermatogenesis and epididymal sperm morphology

Cited by 24 publications

References 60 publications

Normozoospermic versus teratozoospermic domestic cats: differential testicular volume, sperm morphometry, and subpopulation structure during epididymal maturation

Normozoospermic versus teratozoospermic domestic cats: differential testicular volume, sperm morphometry, and subpopulation structure during epididymal maturation

Recovery of sperm after epididymal refrigeration from domestic cats using ACP-117c and Tris extenders

Evaluation of sperm head dimensions and chromatin integrity of epididymal sperm from domestic cats using the toluidine blue technique

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