2016
DOI: 10.1590/1678-4162-8653 View full text |Buy / Rent full text
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Abstract: We aimed to compare fresh sperm and sperm cooled to 4ºC that had been recovered from the epididymides of cats using powdered coconut water (ACP-117c) and Tris extenders. Sixty epididymides were divided into 6 groups: 10 fresh epididymides were recovered using Tris (T0h); 10 were kept at 4°C/2h and recovered using Tris (T2h); 10 were kept at 4°C/4h and recovered using Tris (T4h); 10 fresh were recovered using ACP-117c (A0h); 10 were kept at 4°C/2h and recovered using ACP-117c (A2h), and 10 were kept at 4°C/4h a… Show more

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“…Estudos mostram que o uso do ACP como diluente seminal é uma alternativa promissora e eficaz na conservação de importantes características espermáticas em várias espécies animais [2,9,14,18,22]. Atualmente, poucos são os estudos sobre resfriamento seminal de peixes que envolvem o uso do ACP-104 como diluente, existindo apenas estudos com Prochilodus lineatus [22] e Colossoma macropomum [15], sendo os resultados de cinética semelhantes aos encontrados na presente pesquisa.…”
Section: Discussionunclassified
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“…Estudos mostram que o uso do ACP como diluente seminal é uma alternativa promissora e eficaz na conservação de importantes características espermáticas em várias espécies animais [2,9,14,18,22]. Atualmente, poucos são os estudos sobre resfriamento seminal de peixes que envolvem o uso do ACP-104 como diluente, existindo apenas estudos com Prochilodus lineatus [22] e Colossoma macropomum [15], sendo os resultados de cinética semelhantes aos encontrados na presente pesquisa.…”
Section: Discussionunclassified
“…Atribui-se o sucesso do ACP como diluente seminal à sua composição, com poucos fosfolipídeos e rica em proteínas, sais, açúcares, vitaminas e fatores de crescimento, como o ácido indol-3-acético (AIA) [2]. Estudos demonstraram que o AIA estimula e mantém uma maior motilidade para sêmen de caprinos e ovinos [14], além de proporcionar um maior número de espermatozoides com membrana íntegra [9] e com morfologia normal [2].…”
Section: Discussionunclassified
“…In prepubertal animals, different techniques may be used to obtain sperm, as semen collection with an artificial vagina (Lambo et al., ), electroejaculation (Henry et al., ) or urethral catheterization (Lueders, Luther, Scheepers, & Horst, ), as well as the recovery of epididymal spermatozoa (Lima et al., ). On the other hand, cryopreservation of testicular fragments represents the only possibility to preserve the fertility of prepubertal animals.…”
Section: Introductionmentioning
“…The recovered epididymal was transferred to a 1.5‐ml conical tube, preheated at 37°C. Each sample was previously evaluated for total motility and progressive motility by computer system analysis (CASA), using the Sperm Class Analyser ® software (SCA Microptic SL, version 5.3.0.1), previously configured for the species (Filliers et al,; Lima et al, ).…”
Section: Methodsmentioning