Testicular development, ultrasonographic and histological appearance of the testis in ram lambs immunized against recombinant LHRH fusion proteins

Ülker, Hasan; Kanter, Mehmet; Gökdal, Özdal; Aygün, Turgut; Karakuş, Ferda; Sakarya, Mehmet Emin; deAvila, D M; Reeves, Jerry J.

doi:10.1016/j.anireprosci.2004.07.011

Cited by 33 publications

(27 citation statements)

References 29 publications

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“…The suppression of reproductive function using an anti-GnRF vaccine has been demonstrated in numerous studies in cattle [3][4][5][6][7][8][9][10][11][12][13], sheep [10,[14][15][16][17][18][19][20][21], pig [22][23][24][25][26][27][28], horse [29][30][31][32][33][34] and in wild animals [35][36][37][38][39].…”

Section: Introductionmentioning

confidence: 99%

Vaccination against gonadotropin-releasing factor (GnRF) with Bopriva significantly decreases testicular development, serum testosterone levels and physical activity in pubertal bulls

Janett

Gerig

Tschuor³

et al. 2012

Theriogenology

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The aim of this study was to evaluate the effects of vaccination against gonadotropin-releasing factor (GnRF) on testicular development, testosterone secretion, and physical activity in pubertal bulls. The experiment was performed using 44 bulls aged between 6 and 7 mo. Twenty-three animals were vaccinated twice 4 wk apart with 1 mL of Bopriva (Pfizer, Animal Health, Parkville, Australia) and 21 bulls served as matched controls. Serum GnRF antibody titer and testosterone concentration as well as body weight and scrotal circumference were determined in all bulls for 24 wk from the first vaccination. In addition, physical activity was analyzed in 11 vaccinated and in 10 control animals using the ALPRO DeLaval activity meter system (DeLaval AG, Sursee, Switzerland). The results show that vaccination significantly (P < 0.05) influenced all parameters evaluated except body weight. Antibody titers to GnRF began to rise 2 wk after the first vaccination and reached peak values 2 wk after the second injection. Significant group differences in anti-GnRF titer were present for 22 wk following the first vaccination. Testosterone concentrations were significantly lower between weeks 6 to 24 after first vaccination in bulls with Bopriva compared with control animals. In vaccinated bulls testicular development was impaired after the second injection and scrotal circumference was significantly smaller between weeks 8 to 24 after first vaccination. Physical activity of vaccinated bulls was reduced after the booster injection with significant group differences for a continuous period of 106 days. In conclusion, vaccination against GnRF with Bopriva in pubertal bulls decreased testosterone levels in peripheral blood, testicular development, and physical activity but did not affect weight gain. AbstractThe aim of this study was to evaluate the effects of vaccination against gonadotropinreleasing factor (GnRF) on testicular development, testosterone secretion and physical activity in pubertal bulls. The experiment was performed using 44 bulls aged between 6 and 7 months. Twenty-three animals were vaccinated twice 4 weeks apart with 1 mL of Bopriva ® (Pfizer Animal Health, Australia) and 21 bulls served as matched controls. Serum GnRF antibody titer and testosterone concentration as well as body weight and scrotal circumference were determined in all bulls for 24 weeks from the first vaccination. In addition, physical activity was analyzed in 11 vaccinated and in 10 control animals using the ALPRO  DeLaval activity meter system (DeLaval AG, Sursee, Switzerland). The results show that vaccination significantly (P < 0.05) influenced all parameters evaluated except body weight. Antibody 2 titers to GnRF began to rise 2 weeks after the first vaccination and reached peak values 2 weeks after the second injection. Significant group differences in anti-GnRF titer were present for 22 weeks following the first vaccination. Testosterone concentrations were significantly lower between weeks 6 to 24 after first vaccination in bulls w...

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Section: Introductionmentioning

confidence: 99%

Vaccination against gonadotropin-releasing factor (GnRF) with Bopriva significantly decreases testicular development, serum testosterone levels and physical activity in pubertal bulls

Janett

Gerig

Tschuor³

et al. 2012

Theriogenology

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“…The reproductive status of various animals, including rams, rabbits, and cetaceans, has been evaluated using testicular echodensity [23,30,31]. Even though tissue densities and tissue characteristics were assessed ultrasonographically, these characteristics were not readily quantifiable.…”

Section: Discussionmentioning

confidence: 99%

B-Mode ultrasonographic evaluation of the testis in relation to serum testosterone concentration in male Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis) during the breeding season

Wu¹,

Hao²,

Li³

et al. 2010

Theriogenology

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“…Active immunization against GnRH and its analogues has a significant effect on fertility in males and females of different species (7)(8)(9)(10). Immune neutralization of GnRH decreased gonadotroph hormones and sex steroids, with eventual gonadal atrophy.…”

Section: Discussionmentioning

confidence: 99%

Histological Investigation of Testicular and Accessory Sex Glands in Ram Lambs Immunized Against Recombinant GNRH Fusion Proteins

Odabaş

Kanter

2008

ELECTRON J GEN MED

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Aim:The aim of the study was to investigate the histological appearance of testis, epididymis, prostate and seminal vesicles of ram lambs immunized against gonadotrophin releasing hormone (GnRH).Methods: The study was performed on 14 male lambs, 7 in the study group and the other 7 in the control group. Another 5 lambs were included from the study after biopsy samples were taken from their testes at age 10 weeks to determine testicular histology at the start of the study. Seven male lambs aged 10 weeks were immunized against GnRH using a recombinant GnRH fusion protein vaccine. The immunization was repeated twice monthly until slaughter at 9 months of age. Testes and epididymis biopsies were taken at the 6th month. After slaughter, testis, epididymis, prostate and seminal vesicles were extracted for histopathological investigation.Results: At the 6th month and at slaughter histopathology of all the sex glands, most evidently in the testes, indicated secondary infertility. In the study group, tubule diameter decreased and spermatogenetic activity disappeared. The ball of sperm cells seen in the epididymis tubules of the control group at the 9th month was not seen in the study group. Changes in prostate tissues between the study and control groups were not evident but stromal tissue in the seminal vesicles increased in the study group.Conclusion: In conclusion, the histomorphological changes showed that recombinant GnRH fusion proteins provided immune castration in male lambs when used at an early age.

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Testicular development, ultrasonographic and histological appearance of the testis in ram lambs immunized against recombinant LHRH fusion proteins

Cited by 33 publications

References 29 publications

Vaccination against gonadotropin-releasing factor (GnRF) with Bopriva significantly decreases testicular development, serum testosterone levels and physical activity in pubertal bulls

Vaccination against gonadotropin-releasing factor (GnRF) with Bopriva significantly decreases testicular development, serum testosterone levels and physical activity in pubertal bulls

B-Mode ultrasonographic evaluation of the testis in relation to serum testosterone concentration in male Yangtze finless porpoise (Neophocaena phocaenoides asiaeorientalis) during the breeding season

Histological Investigation of Testicular and Accessory Sex Glands in Ram Lambs Immunized Against Recombinant GNRH Fusion Proteins

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