Teste de tetrazólio para as sementes de milho: períodos de hidratação e de coloração das sementes

Chamma, Helena Maria Carmignani Pescarin; Novembre, Ana Dionisia da Luz Coelho

doi:10.1590/s0101-31222007000200017

Cited by 14 publications

(6 citation statements)

References 3 publications

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“…Diversos autores têm abordado a adequação da metodologia do teste para várias espécies, como Oliveira et al (2005), em sementes de canafístula; Chamma & Novembre (2007), em milho; Kak et al (2009) e Pinto et al (2009, em pinhão-manso; Souza et al (2010), em triticale; Costa & Santos (2010), em leucena; e Clemente et al (2011) (Castro & Farias, 2005), e os resultados foram expressos em percentagem de plântulas emergidas. Para determinação das classes de vigor das sementes, foram utilizadas 400 sementes por lote, submetidas à melhor combinação de pré-condicionamento e coloração obtida na etapa anterior do experimento, ou seja: pré-condicionamento por imersão direta em água durante 16 horas, a 25°C, e coloração por imersão na solução de tetrazólio a 0,1%, durante 3 horas, a 30°C.…”

Section: Introductionunclassified

Adaptação do teste de tetrazólio para avaliação da viabilidade e do vigor de sementes de girassol

Silva

Grzybowski

Neto

et al. 2013

Pesq. agropec. bras.

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Resumo -O objetivo deste trabalho foi avaliar metodologias para realização do teste de tetrazólio, na avaliação da viabilidade e do vigor de sementes de girassol, e estabelecer classes de qualidade para interpretação do teste. Foram avaliadas diferentes formas de extração do pericarpo e do tegumento das sementes, além do pré-condicionamento em água por 16 e 18 horas (25°C) e da coloração em solução de tetrazólio (30ºC), nas concentrações de 0,1 e 0,5%, por 2, 3 e 4 horas; e de 1,0%, por 1, 2 e 3 horas. Após a definição da metodologia mais adequada para realização do teste, classes de vigor foram estabelecidas de acordo com a emergência das plântulas no campo. Foram identificadas cinco classes, as quais apresentaram correlação perfeita com a emergência. Para avaliar a viabilidade das sementes de girassol, o pericarpo e o tegumento devem ser retirados após corte longitudinal com até 1/3 do comprimento da semente, o pré-condicionamento deve ser feito pela imersão em água por 16 horas, e a coloração deve ser realizada em solução de tetrazólio a 0,1%, durante 3 horas.Termos para indexação: Helianthus annuus, dormência, qualidade fisiológica. Adaptation of the tetrazolium test for assessment of sunflower seed viability and vigorAbstract -The objective of this work was to evaluate methodologies for performing the tetrazolium test in the evaluation of the viability and vigor of sunflower seeds, and to establish seed quality classes for test interpretation. Different methodologies were evaluated for extracting the pericarp and seed coat, besides water priming for 16 and 18 hours (25°C), and staining with tetrazolium solution (30ºC) at the concentrations of 0.1 and 0.5%, for 2, 3, and 4 hours; and of 1.0%, for 1, 2, and 3 hours. After the definition of the most adequate methodology for performing the test, vigor classes were established according to seedling emergence on field. Five vigor classes were established, which had perfect correlation with seedling emergence. For the viability assessment of sunflower seeds, the pericarp and the seed coat should be removed after a lengthwise cut of up to 1/3 seed length, water priming should be carried out for 16 hours, and staining should be done with tetrazolium solution at 0.1%, during 3 hours.

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Section: Introductionunclassified

Adaptação do teste de tetrazólio para avaliação da viabilidade e do vigor de sementes de girassol

Silva

Grzybowski

Neto

et al. 2013

Pesq. agropec. bras.

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“…Also, it is an alternative for species whose seeds have dormancy or slow germination, especially native and forest species (Lamarca and Barbedo, 2014). It has also been used efficiently in seeds from agricultural crops such as soybean (Pereira et al, 2019), chickpeas (Paraíso et al, 2019), beans (Bhering et al, 1999), cotton (Santos et al, 1992), corn (Chamma and Novembre, 2007), coffee (Dias and Silva, 1986) and barley (Grzybowski et al, 2012). In forage species such as Brachiaria spp.…”

Section: Introductionmentioning

confidence: 99%

Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds

et al. 2019

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The tetrazolium test has been recommended for evaluating the viability of seeds with slow and ununiform germination, especially agroforestry species. The aim of this study was to propose a methodology to evaluate the viability of J. curcas L. seeds using the tetrazolium test and to generate visual aids to support the interpretation of the test. In the first trial, methods of seed preparation and staining were evaluated. The most promising methodologies were used to conduct the second test, which consisted of the evaluation of different concentrations (0.10 and 0.25%) and times (2 and 3 h) of immersion of the seeds in the tetrazolium solution, using five lots. The seeds were analyzed individually and classified as viable or non-viable. At the same time, germination, first count, emergence and seedling emergence speed index tests were conducted. The tetrazolium test is efficient for evaluating the viability of seeds of J. curcas L. providing results correlated with germination and seedling emergence. Pre-soaked seeds should have their integument removed and then sectioned longitudinally and medially lengthwise for immersion in 0.10% solution of 2,3,5-triphenyl chloride tetrazolium for three hours at 35 °C.

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“…This method was later improved by adding a parameter called the black layer which is a thin layer in the kernel basal area that develops simultaneously to physiological maturity [3]. Other researchers also studied the black layer and added information about the correlation between seed water content, dry matter, period of formation and environmental influence [3][4][5][6][7][8]. Following, researchers related the solidification of the endosperm through the seed from the top to the base and created a new parameter, the milk line [9].…”

Section: Introduction mentioning

confidence: 99%

Physiological Maturity of Corn Seeds (Zea mays) by Tetrazolium Salt

Torrezan¹,

Novembre²

2018

JFSE

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The purpose of this study was to evaluate the relationship of tetrazolium salt testing to common indicators (milk line, black layer, dry matter) used to determine corn seed physiological maturity. The seeds used were the hybrids Pioneer 4285 and Dow 2B587. These hybrids were harvested 40 days after polinization (DAP), with 4-day intervals until 68 DAP. The harvested seeds were evaluated for viability and vigor (germination test, accelerated aging test and cold test). The parameters used to determine the physiological seed's maturity were the black layer, the milk line, the seed dry matter, seed water content and the evaluation of seed tissues using the tetrazolium salt to evaluate the endosperm cells. The germination of both materials was greater than 95% and there was no difference between harvest times. Corn seed physiological maturity (PM) was identified at 56 DAP for P4285 (F2) and between 48 and 56 DAP for 2B587 (F2). The maximum vigor was detected by accelerated aging tests and cold tests before PM for the two genotypes. The activity of endosperm cells is related to the other PM indicators (milk line, black layer, dry matter and water content). The transport of nutrients from plant to the seed ceases at seed physiological maturity. Use of tetrazolium salt allows identifying cell death of the basal region and this parameter corresponds to the others evaluated, thus demonstrating that the tetrazolium salt is effective for corn seed physiological maturity characterization.

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Teste de tetrazólio para as sementes de milho: períodos de hidratação e de coloração das sementes

Cited by 14 publications

References 3 publications

Adaptação do teste de tetrazólio para avaliação da viabilidade e do vigor de sementes de girassol

Adaptação do teste de tetrazólio para avaliação da viabilidade e do vigor de sementes de girassol

Adequacy of the tetrazolium test to evaluate the viability of Jatropha curcas L. seeds

Physiological Maturity of Corn Seeds (Zea mays) by Tetrazolium Salt

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