Test-strip method for measuring lactate in whole blood.

Shimojo, Nobuo; Naka, Keiichi; Nakajima, Chiaki; Yoshikawa, Chikao; Okuda, Kiyoshi; Okada, Kunio

doi:10.1093/clinchem/35.9.1992

Cited by 76 publications

(22 citation statements)

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“…Iron (Fe) was determined using the photometric method with ferene (ferroin‐type reagent) without deproteination (Higgins 1981). The concentration of lactate (LACT) in plasma was measured using the enzymatic method according to Shimojo, Nakajima, Yoshikawa, Okuda and Okada (1989). Albumin (ALB) was determined using the photometric method with bromocresol green (Doumas, Watson & Biggs 1971).…”

Section: Methodsmentioning

confidence: 99%

Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpioL.) from a hypertrophic pond with cyanobacterial water bloom

Kopp

Mareš

Palíková

et al. 2009

Aquaculture Research

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The aim of this study was to evaluate the dynamics of the blood plasma parameters and the content of microcystins in the tissues of common carp (Cyprinus carpio L.) in relation to the toxic cyanobacterial water bloom. Fish (average body mass 2176 AE 697 g) in the hypertrophic pond were exposed to natural water bloom (dominated by Planktothrix agardhii, Pseudanabaena limnetica and Limnothrix redekei), which contained microcystins (concentration in biomass 20^181 mg g À 1 dry wt, concentration in water 0.3^9.5 mg L À 1 ). Biochemical parameters in ¢sh blood plasma were analysed in 89 ¢sh at 14-day intervals during the whole season (nine sampling periods). Our results demonstrated high variability and £uctuations in the investigated parameters. The content of microcystins and density of cyanobacterial cells correlated with some haematological indices as lipase, alanine^aminotransferase, albumin, magnesium and chlorides. The concentrations of microcystins in the muscle and liver of the ¢sh (determined by high-performance liquid chromatography with mass spectrometer) were below the limit of detection during the monitored period [0.31ng g À 1 fresh weight (f.w.) for the liver and 0.13 ng g À 1 f.w. for muscle]. Our study demonstrates that although known cyanobacterial toxin microcystins were not detected in the ¢sh tissues, several biochemical parameters important for the ¢sh physiology were modulated by natural cyanobacterial bloom.Results show two-way Spearman's R with the P-value in parentheses (presented are only the combinations of parameters that demonstrated statistical signi¢cance, Po0.05). NS, nonsigni¢cant correlation.Aquaculture Research, 2009 Biochemical parameters and microcystins in carp R Kopp et al.

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Section: Methodsmentioning

confidence: 99%

Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpioL.) from a hypertrophic pond with cyanobacterial water bloom

Kopp

Mareš

Palíková

et al. 2009

Aquaculture Research

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“…Detailed information on principles of the POC lactate meters is described elsewhere. 11,12 Statistical Analysis Data were first subjected to the PROC MEANS and UNI-VARIATE procedures to determine descriptive statistics parameters (mean, range, standard deviation, range, median, and percentiles) as well as to assess normality. h One observation was an outlier and was deleted from the dataset.…”

Section: Blood L-lactate Measurement By the Poc Lactate Metersmentioning

confidence: 99%

Evaluation of 4 Point‐of‐Care Units for the Determination of Blood l‐Lactate Concentration in Cattle

Karapinar

Kaynar

Hayırlı

et al. 2013

Veterinary Internal Medicne

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Background: Despite being used commonly in bovine medicine, information on reliability of point-of-care (POC) lactate meters is limited.Objective: To determine the validity of 4 commercially available POC lactate meters in cattle. Animals: Cattle with various diseases (n = 120).Methods: Blood samples collected from the jugular vein were processed immediately on POC lactate meters. Plasma Llactate concentration was measured by the enzymatic-colorimetric method (ELISA). Data were subjected to Friedman's test for comparison, Passing-Bablok regression and Bland-Altman plot analyses for reliability, and receiver operating characteristics analysis for sensitivity (Se) and specificity (Sp).Results: The POC lactate meters were highly correlated with ELISA (r = 0.98-0.99) despite disagreements among units. When regressed on ELISA, blood L-lactate concentrations generated from Accutrend Plus and Lactate Pro were linear up to 16.6 and 15.7 mmol/L, respectively, whereas those generated from i-STAT and Lactate Scout were linear up to 19.5 and 19.7 mmol/L, respectively. All POC lactate meters had a Se of 100% with Sp of 95.7-98.6% at a plasma L-lactate cut-off concentration of 4 mmol/L. i-STAT had the best accuracy (99.0%) and precision (99.8%), the best linear fit (y = À0.13 + 1.04X) yielding the lowest bias (À6.6%) as well as the highest Se (100%) and Sp (98.6%).Conclusions and Clinical Importance: Despite high correlation with the reference method, dilution is needed for Accutrend Plus/Lactate Pro and i-STAT/Lactate Scout if concentrations >15 and 20 mmol/L, respectively. i-STAT provided the most accurate and precise results.

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“…In the present study we assessed a new and simple dry reagent strip method for measuring lactate in fetal whole blood (Shimojo et al 1989). The method takes only 1 min and requires only 20 μ1 of blood.…”

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confidence: 99%

Fetal scalp and umbilical artery blood lactate measured with a new test strip method

Nordström

Persson

Shimojo

et al. 1992

BJOG

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Objective To compare the measurement of lactate in fetal scalp and umbilical artery blood by a new dry reagent strip method with a commercially available enzymatic method using plasma (Monotest). Design Comparative study. Subjects Fetal scalp blood samples were obtained during labour from 24 fetuses and umbilical artery blood samples were obtained at birth in a further 51 deliveries. Results The concentration of lactate in scalp and umbilical artery blood measured by the reagent strip method correlated well (r= 0.94, P<0.001 and 0.95, P<0.001 respectively) with the enzymatic plasma method. The paper strip method tended to give lower readings than the enzymatic method when the fetal haematocrit was ≥50%. Conclusions The new dry reagent strip method which takes only 1 min to carry out and requires only 20 μl of blood seems to be handy and reliable. This system provides a convenient and rapid test for measuring fetal blood lactate.

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Test-strip method for measuring lactate in whole blood.

Cited by 76 publications

References 0 publications

Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpioL.) from a hypertrophic pond with cyanobacterial water bloom

Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpioL.) from a hypertrophic pond with cyanobacterial water bloom

Evaluation of 4 Point‐of‐Care Units for the Determination of Blood l‐Lactate Concentration in Cattle

Fetal scalp and umbilical artery blood lactate measured with a new test strip method

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