Biochemical parameters of blood plasma and content of microcystins in tissues of common carp (Cyprinus carpioL.) from a hypertrophic pond with cyanobacterial water bloom

Abstract

The aim of this study was to evaluate the dynamics of the blood plasma parameters and the content of microcystins in the tissues of common carp (Cyprinus carpio L.) in relation to the toxic cyanobacterial water bloom. Fish (average body mass 2176 AE 697 g) in the hypertrophic pond were exposed to natural water bloom (dominated by Planktothrix agardhii, Pseudanabaena limnetica and Limnothrix redekei), which contained microcystins (concentration in biomass 20^181 mg g À 1 dry wt, concentration in water 0.3^9.5 mg L À 1 ). Biochemical parameters in ¢sh blood plasma were analysed in 89 ¢sh at 14-day intervals during the whole season (nine sampling periods). Our results demonstrated high variability and £uctuations in the investigated parameters. The content of microcystins and density of cyanobacterial cells correlated with some haematological indices as lipase, alanine^aminotransferase, albumin, magnesium and chlorides. The concentrations of microcystins in the muscle and liver of the ¢sh (determined by high-performance liquid chromatography with mass spectrometer) were below the limit of detection during the monitored period [0.31ng g À 1 fresh weight (f.w.) for the liver and 0.13 ng g À 1 f.w. for muscle]. Our study demonstrates that although known cyanobacterial toxin microcystins were not detected in the ¢sh tissues, several biochemical parameters important for the ¢sh physiology were modulated by natural cyanobacterial bloom.Results show two-way Spearman's R with the P-value in parentheses (presented are only the combinations of parameters that demonstrated statistical signi¢cance, Po0.05). NS, nonsigni¢cant correlation.Aquaculture Research, 2009 Biochemical parameters and microcystins in carp R Kopp et al.

Dietary zinc requirement of Siberian sturgeon (Acipenser baerii, Brandt 1869) juveniles, based on the growth performance and blood parameters

“…Although the normal ranges for biochemical key parameters have been identified for various raised fish species (Kopp et al 2009;Xiaoyun et al 2009;Satheeshkumar et al 2011;Liang et al 2012), there is scare information about the blood characteristics in Siberian sturgeon especially under culture conditions. Results of the present study illustrated that supplemental dietary Zn improves the hematological parameters of Siberian sturgeon.…”

Relationships among cyanobacteria, zooplankton and fish in sub-bloom conditions in the Sulejow Reservoir

“…However, in the case of the trout mortality observed at Loch Leven, healthy levels of oxygen were maintained in the lake, but severe liver damage after the lysis of a toxic Dolichospermum (Anabaena) flos-aquae (Brébisson ex Bornet & Flahault) bloom was observed, a reaction similar to that of fish administered MC extracts (Rodger et al, 1994). The negative responses of fish exposed to cyanotoxins, especially MCs, are not limited to hepatotoxicity, but also include nephrotoxicity, immunotoxicity or changes in biochemical and hematological parameters (Rodger et al, 1994;Kotak et al, 1996;Malbrouck and Kestemont, 2006;Chen et al, 2009;Kopp et al, 2009;Qiu et al, 2009;Sieroslawska et al, 2012). Developmental processes and behaviors can also be disturbed (Oberemm et al, 1999;Baganz et al, 2004;Wang et al, 2005).…”

Simultaneous accumulation of anatoxin-a and microcystins in three fish species indigenous to lakes affected by cyanobacterial blooms

“…Despite the extensive investigation of cyanobacterial metabolites (Carmichael 1992, Mazur-Marzec 2006, Welker and Döhren 2006 and their influence on living organisms (Malbrouck andKestemont 2006, Wilson et al 2006), there is still insufficient information on their accumulation, effects and transfer in aquatic food webs. Only in recent years, the influence of microcystins on ichthyofauna of European waters has been investigated (Ibelings et al 2005, Kopp et al 2009, Papadimitriou et al 2010. However, there is still very limited information on ANTX accumulation in fish in natural, although polluted, environment.…”

“…Recently, the influence of secondary metabolites of cyanobacteria (including hepatotoxic microcystins and neurotoxic anatoxin-a) on ichthyofauna attracts the increasing interest due to their possible impact on human health and negative economic consequences (Welker, von Dohren 2006;Malbrouck , Kestemont 2006;Smith et al 2008;Kopp et al 2009;Papadimitriou et al 2010). Microcystins and other less known metabolites of freshwater cyanobacteria are regarded as developmental toxins inhibiting different stages of fish embryogenesis (Berry et al 2007).…”

“…Accumulation of microcystins and their influence on some fish species (e.g. Danio rerio, Carassius carpio, Cyprinus carpio) were studied mainly under laboratory conditions (Malbrouck , Kestemont 2006;Kopp et al 2009;El Ghazali Issam et al 2010). However, there are very limited field studies on the dynamics of cyanotoxins (especially anatoxin-a) accumulation in ichthyofauna indigenous to Eurasian waters, which can be used as a food source.…”