Test-Retest Reliability of Pediatric Heart Rate Variability

Abstract

Heart rate variability (HRV), an established index of autonomic cardiovascular modulation, is associated with health outcomes (e.g., obesity, diabetes) and mortality risk. Time-and frequencydomain HRV measures are commonly reported in longitudinal adult and pediatric studies of health. While test-retest reliability has been established among adults, less is known about the psychometric properties of HRV among infants, children, and adolescents. The objective was to conduct a meta-analysis of the test-retest reliability of time-and frequency-domain HRV measures from infancy to adolescence. Electronic searches (PubMed, PsycINFO; January 1970-December 2014 identified studies with nonclinical samples aged ≤ 18 years; ≥ 2 baseline HRV recordings separated by ≥ 1 day; and sufficient data for effect size computation. Forty-nine studies (N = 5,170) met inclusion criteria. Methodological variables coded included factors relevant to study protocol, sample characteristics, electrocardiogram (ECG) signal acquisition and preprocessing, and HRV analytical decisions. Fisher's Z was derived as the common effect size. Analyses were age-stratified (infant/toddler < 5 years, n = 3,329; child/adolescent 5-18 years, n = 1,841) due to marked methodological differences across the pediatric literature. Meta-analytic results revealed HRV demonstrated moderate reliability; child/adolescent studies (Z = 0.62, r = 0.55) had significantly higher reliability than infant/toddler studies (Z = 0.42, r = 0.40). Relative to other reported measures, HF exhibited the highest reliability among infant/toddler studies (Z = 0.42, r = 0.40), while rMSSD exhibited the highest reliability among child/adolescent studies (Z = 1.00, r = 0.76). Moderator analyses indicated greater reliability with shorter test-retest interval length, reported exclusion criteria based on medical illness/condition, lower proportion of males, prerecording acclimatization period, and longer recording duration; differences were noted across age groups. HRV is reliable among pediatric samples. Reliability is sensitive to pertinent methodological decisions that require careful consideration by the researcher. Limited methodological reporting precluded several a priori moderator analyses. Suggestions for future research, including standards specified by Task Force Guidelines, are discussed. Keywordspediatric; heart rate variability; reliability; respiratory sinus arrythmia; psychometric CIHR Author Manuscript CIHR Author Manuscript CIHR Author ManuscriptHeart rate variability (HRV) reflects the variance in time between consecutive sinoatrial depolarizations (i.e., NN-intervals) and is an established index of autonomic cardiovascular modulation. Commonly reported time-and frequency-domain HRV measures, typically derived using continuous electrocardiogram (ECG) recordings and specialized analysis software programs, each provide a unique and nuanced perspective of autonomic functioning (Ernst, 2014;Kleiger, Stein, & Bigger, 2005).Mathematically derived time-domain measures inc...

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