Test performance of three serological assays for the detection of Mycobacterium bovis infection in common warthogs (Phacochoerus africanus)

Roos, Eduard O.; Buss, Peter; Klerk‐Lorist, Lin‐Mari de; Hewlett, Jennie; Hausler, Guy; Rossouw, Leana; McCall, Alicia J.; Cooper, David; Helden, Paul D. van; Parsons, Sven D.C.; Miller, Michele A.

doi:10.1016/j.vetimm.2016.10.006

Cited by 27 publications

(45 citation statements)

References 29 publications

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“…Blood was collected into vacutainers and serum harvested as previously described (Roos et al., ). Post‐mortem examinations were performed on a subset of the warthogs from each population; MK ( n = 21), MP ( n = 24), SK ( n = 5) and SA ( n = 19).…”

Section: Methodsmentioning

confidence: 99%

“…The indirect PPD ELISA and TB ELISA‐VK ® were used to test warthog serum samples as previously described (Roos et al., ). All warthogs were tested using the indirect PPD ELISA, with a subset of 100 animals tested using the TB ELISA‐VK ® .…”

Section: Methodsmentioning

confidence: 99%

“…The serological assays had high sensitivities (75%–88%) and high proportions of test negative animals (79%–89%), suggesting that these tests could be used to accurately measure the prevalence of M . bovis infection in warthogs (Roos et al., ). Serological assays have further potential value in field surveillance as both retrospective and prospective samples can be used (Boadella et al., ; Chambers, ; Maas et al., ).…”

Section: Introductionmentioning

confidence: 99%

“…Serological assays have further potential value in field surveillance as both retrospective and prospective samples can be used (Boadella et al., ; Chambers, ; Maas et al., ). Determining prevalence and risk factors will contribute to our understanding of the role that warthogs play in the ecosystem with regards to bTB (Boadella et al., ; Roos et al., ). Identification of potential maintenance hosts is crucial to inform management and control strategies.…”

Section: Introductionmentioning

confidence: 99%

“…However, novel diagnostic tests for warthogs are required to investigate the epidemiology of bTB in this species. Recent studies have demonstrated that serological assays are capable of discriminating between M. bovis ‐infected and uninfected warthogs (Roos et al., ). In this study, an indirect ELISA utilizing M. bovis purified protein derivative ( PPD ) as a test antigen was used to measure the prevalence and investigate risk factors associated with infection in warthogs from uM hkuze Nature Reserve and the southern region of the Greater Kruger National Park ( GKNP ).…”

mentioning

confidence: 99%

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Seroprevalence ofMycobacterium bovisinfection in warthogs (Phacochoerus africanus) in bovine tuberculosis-endemic regions of South Africa

Roos

Olea‐Popelka

Buss

et al. 2018

Transbound Emerg Dis

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Bovine tuberculosis (bTB), caused by Mycobacterium bovis (M. bovis), has been reported in many species including suids. Wild boar are important maintenance hosts of the infection with other suids, that is domestic and feral pigs, being important spillover hosts in the Eurasian ecosystem and in South Africa, warthogs (Phacochoerus africanus) may play a similar role in M. bovis-endemic areas. However, novel diagnostic tests for warthogs are required to investigate the epidemiology of bTB in this species. Recent studies have demonstrated that serological assays are capable of discriminating between M. bovis-infected and uninfected warthogs (Roos et al., ). In this study, an indirect ELISA utilizing M. bovis purified protein derivative (PPD) as a test antigen was used to measure the prevalence and investigate risk factors associated with infection in warthogs from uMhkuze Nature Reserve and the southern region of the Greater Kruger National Park (GKNP). There was a high overall seroprevalence of 38%, with adult warthogs having a higher risk of infection (46%). Seroprevalence also varied by geographic location with warthogs from Marloth Park in the GKNP having the greatest percentage of positive animals (63%). This study indicates that warthogs in M. bovis-endemic areas are at high risk of becoming infected with mycobacteria. Warthogs might present an under-recognized disease threat in multi-species systems. They might also serve as convenient sentinels for M. bovis in endemic areas. These findings highlight the importance of epidemiological studies in wildlife to understand the role each species plays in disease ecology.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%