Mycobacterium bovis prevalence affects the performance of a commercial serological assay for bovine tuberculosis in African buffaloes

Heijden, E. M. D. L. van der; Cooper, David; Rutten, Victor P.M.G.; Michel, Anita Luise

doi:10.1016/j.cimid.2019.101369

Cited by 14 publications

(21 citation statements)

References 56 publications

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“…Studies have shown that while working in slaughterhouses, workers are covered in blood and cattle droppings with the potential risk of contamination (33)(34)(35)(36). The use of an apron, waterproof gloves and the wearing of a visor would limit the circumstances of accidental contact with the skin.…”

Section: Resultsmentioning

confidence: 99%

Risk Factors for the Transmission of Mycobacterium Bovis to Slaughterhouses Workers in Brazzaville, Republic of the Congo

Miassangoumouka¹,

Linguissi²,

Itoua³

et al. 2020

Preprint

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Zoonotic tuberculosis is a major form of TB affecting humans and many animal species. Infected cattle pose a threat to public health. The aim of the study was to characterize the risks of exposure to bovine tuberculosis by slaughterhouse workers in Brazzaville. A survey was carried out among 57 workers from three slaughterhouses in Brazzaville (Mpila, Kanga Mbanzi and La Fougère) using questionnaires and field observations. The results of the profile revealed that the majority of staff are made up mainly of people over 40 years old (40.6 ± 8.4) i.e. 50.9% and 96.5% male. Married workers represent a third of respondents (29.8%) and live in families made up of 4.9 ± 2.4 people. Workers with secondary education are the majority (66.7%). The average number of years of work in slaughterhouses in Brazzaville is 18.9 ± 9.5 years. The survey also showed that many workers, 52.6%, have never received training to work in slaughterhouses. A tier of workers (31.7%) have no knowledge of animal tuberculosis, in particular its modes of transmission or its clinical signs. The premises are; normal, regularly cleaned, but are not disinfected. Despite the common occurrence of injuries, 73.7% of those surveyed said that they were not subjected to medical checks. To this, we must add, the lack of work clothes adapted by some (29.8%). All of these shortcomings constitute significant risks of contamination from zoonoses, and tuberculosis in particular. Thus, the study recommends strengthening the capacities of professionals in slaughterhouses, their medical follow-up and improve infrastructure. Finally, the fight against zoonotic tuberculosis requires a multisectoral framework involving doctors and veterinarians in the context of the "One Health" approach.

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Section: Resultsmentioning

confidence: 99%

Risk Factors for the Transmission of Mycobacterium Bovis to Slaughterhouses Workers in Brazzaville, Republic of the Congo

Miassangoumouka¹,

Linguissi²,

Itoua³

et al. 2020

Preprint

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“…The evaluation of experimental diagnostic techniques for the detection of antibodies against M. bovis has demonstrated that depending on the epidemiological situation the Se of the antibody detection tests in the absence of the booster effect could be even bigger than the one obtained using o cial techniques that detect the cell-mediated immune (CMI) response (27,28). Recently, under a high bTB prevalence situation, serological tests presented higher Se than o cial techniques in the absence of booster effect (17,29). In our study, adopting a parallel interpretation the apparent prevalence ranged from 1.25% − 11.25% within the herds and may explain the higher Se presented by ELISA IDEXX™.…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosismentioning

confidence: 99%

“…The lack of or low agreement between the positive results of the two tests may re ect different elements of the immune response (humoral and cell-mediated immunity) (33,34). The detection of CMI response to infection with M. bovis, as assessed by the TST usually fail to detect chronic stages of the infection (26,29,35).…”

Section: Prevalence Of Tuberculin Skin Test X Elisa For the Diagnosismentioning

confidence: 99%

“…Over the last few years, the potential for use of an antibody assays to detect M. bovis infection in cattle is being consolidated (16,17,18,19,20,21,22,23,24,25). The ELISA have proven to be useful as ancillary serial (to enhance Sp) and parallel (to enhance Se) tests in several species (26,27,28,29,30). Moreover, a booster effect on the antibody response caused after injection of tuberculin has been reported and recommended as a strategic option to increase the sensitivity of serological assays (26,27,28).…”

Section: Introductionmentioning

confidence: 99%

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Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Carneiro

Sousa

Viana

et al. 2020

Preprint

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Background With a worldwide occurrence, bovine tuberculosis (bTB) is a zoonotic disease that is difficult to control, mainly due to the lack of a diagnostic testing to detect infected animals at all stages. Furthermore, the current standard diagnostic test, the Tuberculin Skin Test (TST), is logistically difficult and time consuming. To address this challenge, the aim of this study was to evaluate the sensitivity and specificity of a multiplex enzyme-linked immunosorbent assay (ELISA) comparing with the TST used for the diagnosis of tuberculosis in cattle in Brazil. The study included 400 Nelore females raised for beef on five farms, in different municipalities in Brazil. The comparative cervical test (CCT) was done and on the day of inoculation of the Purified Protein Derivative (PPD) blood samples were obtained and stored for further analysis of the ELISA IDEXX™ Mycobacterium bovis immunoassay. Results Lack of agreement between CCT and ELISA IDEXX™ was observed. No diagnosis described as positive reactor on the CCT was positive at the ELISA, indicating two false positive reactors and 22 negative reactors by CCT were positive by the ELISA IDEXX™. The ELISA IDEXX™ showed sensitivity significantly higher than the official CCT and no significant differences in specificity was observed. ELISA also detected infected animals and herds undetected by the CCT. The parallel use of CCT and ELISA increased sensitivity and the feasibility bTB screening, thus improving the cleaning of the herds. Conclusions The results obtained here suggest that the ELISA IDEXX™ may be a supplemental test for the detection of Mycobacterium bovis infection in regions without routine testing and slaughter, where the disease generally progresses to more advanced stages and antibody responses are likely to be more prevalent. The results provided evidence to support the validation of the ELISA IDEXX™ as a supplemental test for bTB eradication programs.

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“…However, in the literature, regarding IFN-γ test in buffalo species, scarce data are available and mainly associated with African buffaloes (S. caffer), (34)(35)(36)(37)(38)(39)(40)(41).…”

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confidence: 99%

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Bubalus bubalis) Comparing Four Interpretative Criteria

et al. 2020

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Bovine tuberculosis (bTB) is a worldwide zoonosis that affects many species of domestic and wild animals. Mycobaterium bovis is the main cause of infection in water buffalo (Bubalus bubalis) and bovines and is of great concern for human health and for buffalo producers in Italy. The bTB eradication programme is based on slaughterhouse surveillance and intradermal skin tests. Other in vivo diagnostic methods such as the interferon-gamma (IFN-γ) assay have been developed and are widely used in cattle to accelerate the elimination of bTB positive animals. The present study is the first to assess the use and performance of IFN-γ assays, which is used as an ancillary test for bTB diagnosis in water buffalo, and presents the results of a field-evaluation of the assay from 2012 to 2019 during the buffalo bTB eradication programme in Italy. The study involved 489 buffaloes with a positive result to the single intradermal tuberculin test (SITT). The IFN-γ assays and single intradermal comparative tuberculin test were used as confirmation tests. Then, a total of 458 buffaloes, reared on officially tuberculosis-free (OTF) herds, that were confirmed bTB-free for at least the last 6 years were subjected to IFN-γ testing. Furthermore, to evaluate the IFN-γ test in an OTF herd with Paratuberculosis (PTB) infection, 103 buffaloes were subjected to SITT and IFN-γ test simultaneously. Four interpretative criteria were used, and the IFN-γ test showed high levels of accuracy, with sensitivity levels between 75.3% (CI 95% 71.2–79.0%) and 98.4% (CI 95% 96.7–99.4%) and specificity levels between 94.3% (CI 95% 91.2–96.50%) and 98.5% (CI 95% 96.9–99.4%), depending on the criterion used. Finally, in the OTF herd with PTB infection, in buffalo, the IFN-γ test displayed high specificity values according to all 4 interpretative criteria, with specificity levels between 96.7% (CI 95% 88.4–99.5%) and 100% (CI 95% 96.2–100%), while SITT specificity proved unsatisfactory, with a level of 45.3% (CI 95% 35.0–55.7%). Our results showed that the IFN-γ test in the buffalo species could reach high Sensitivity and Specificity values, and that the level of Sensitivity and Specificity could be chosen based on the interpretative criterion and the antigens used depending on the health status of the herd and the epidemiological context of the territory. The IFN-γ test and the use of different interpretative criteria proved to be useful to implement bTB diagnostic strategies in buffalo herds, with the possibility of a flexible use of the assay.

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Mycobacterium bovis prevalence affects the performance of a commercial serological assay for bovine tuberculosis in African buffaloes

Cited by 14 publications

References 56 publications

Risk Factors for the Transmission of Mycobacterium Bovis to Slaughterhouses Workers in Brazzaville, Republic of the Congo

Risk Factors for the Transmission of Mycobacterium Bovis to Slaughterhouses Workers in Brazzaville, Republic of the Congo

Study on a supplemental test to improve the detection of bovine tuberculosis in individual animals and herds

Field Evaluation of the Interferon Gamma Assay for Diagnosis of Tuberculosis in Water Buffalo (Bubalus bubalis) Comparing Four Interpretative Criteria

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