Tertiary Follicular Growth Wave Dynamics after Oestrus Synchronization and Superovulation in Sheep and Goats

Maracek, I; Krajnicáková, M; Kostecký, M.; Gréserová, G.; Valocký, Igor

doi:10.2754/avb200271040481

Cited by 4 publications

(2 citation statements)

References 12 publications

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“…The onset of the ovulatory wave (day 16.8 ± 1.4) coincided with the first day of the maximum follicular diameter of the last wave in anovulatory ovaries. This day was close to the day of luteolysis, and the occurrence of a recruitment and selection phase of preovulatory follicles following a prostaglandin application, which has been also observed by Maracek et al (2002) in sheep. These results are also in accord with those obtained by Viñoles and Rubianes (1998) in Corriedale sheep.…”

Section: Discussionsupporting

confidence: 74%

Follicular dynamics in Serrana goats

Simões

Almeida

Valentim

et al. 2006

Animal Reproduction Science

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Twenty-two Serrana goats were studied through two successive estrous cycles in order to characterize their follicular dynamics during the breeding season. The ovaries of the goats were scanned daily by realtime ultrasonography and all follicles ≥3 mm were measured and classified. The data were classified by the number of follicular waves per goat to test the hypothesis that temporal and morphological differences between the last follicular wave of an ovary, irrespective of ovulation, will affect the selection of the next ovulatory wave.The mean interovulatory interval was 20.7 ± 1.0 days (mean ± S.D.). Three to five waves per estrous cycle were observed and 61.3% (19/31) of cycles had four waves. In estrous cycles with four waves, the day of onset of the first, second, third and fourth wave was 1.4 ± 1.0, 6.9 ± 1.4, 11.6 ± 1.8 and 16.8 ± 1.6, respectively. No differences (P > 0.05) were found between the day of onset of the first and second waves for estrous cycles with three, four or five waves. However, the day of onset of the third and fourth waves occurred later when the number of waves per estrous cycle increased (P < 0.001). The duration of the interwave interval (time between the day of onset of two consecutive waves) was longer when the second wave was ovulatory. The length of the growth phase (2.4 ± 0.9 days) and size (5.9 ± 0.7 mm) of the dominant follicle in the second wave were lower (P < 0.01) than for the first wave (3.3 ± 1.2 days and 6.6 ± 0.9 mm, respectively) and the fifth wave (4.1 ± 1.2 days and 7.5 ± 1.0 mm, respectively). Within pairs of ovaries, the onset of the last wave occurred later (P < 0.05) and was less variable in ovulatory ovaries (day 16.8 ± 1.4, n = 20) than in anovulatory ovaries (day 15.1 ± 3.7, n = 20). The length of the growing phase was longer (P < 0.001) in the last waves of ovulatory ovaries (3.1 ± 0.9 days) than in the last waves of anovulatory ovaries (1.7 ± 0.8 days). These results support the hypothesis that the day of onset of the ovulatory wave is related to or, at least, conditioned by the luteolysis and the decrease in plasma progesterone. * Corresponding author. Tel.: +35 1259350417; fax: +35 1259350480. J. Simões et al. / Animal Reproduction Science 95 (2006) 16-26 17 In summary, the estrous cycle of Serrana goats is characterized by sequential follicular wave growth with a great variability in their onset and duration, with the exception of the ovulatory wave. The temporal and morphological differences observed in the last wave of estrous cycle provide strong evidence for the role of progesterone in their regulation.

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Section: Discussionsupporting

confidence: 74%

Follicular dynamics in Serrana goats

Simões

Almeida

Valentim

et al. 2006

Animal Reproduction Science

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“…Süperovulasyon uygulaması sonucunda alınacak cevap hayvandan hayvana değişmekte ve birçok faktörden etkilenmektedir. Bu nedenle süperovulasyon, embriyo transfer çalışmalarında başarıyı etkileyen en önemli faktörlerdendir (10,21,25,26,37). Literatürde de süperovulasyon cevapları arasında tam bir uyum belirlenememekle birlikte sunulan çalış-mada süperovulasyon sonuçlarıyla diğer çalışmaların sonuçları arasında önemli bir farklılık görülmemiştir.…”

Section: Tartışma Ve Sonuçunclassified

In vivo elde edilen Saanen keçisi embriyolarının yavaş dondurulması üzerine farklı kriyoprotektanların etkisinin karşılaştırılması

GÜLER¹

2018

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Özet: Bu çalışmada farklı kriyoprotektanların, yavaş dondurma yöntemi ile dondurulup çözdürülmüş Saanen keçisi embriyolarının canlılığı üzerine etkilerinin belirlenmesi amaçlanmıştır. Çalışmanın hayvan materyalini 15 baş Saanen ırkı keçi ve 3 baş teke oluşturdu. Keçilere siklusun dönemi gözetilmeksizin 12 gün süreyle fluorogeston asetat (20 mg) emdirilmiş intravaginal sünger yerleştirildi. Sünger uygulamasının 9. gününden itibaren 3 gün süreyle 12 saat arayla azalan dozlarda folikül uyarıcı hormon (FSH) (50-50; 30-30; 20-20 Comparision of different cryoprotectants on slow freezing of in vivo derived Saanen goats embryosSummary: This study aimed to determine the effects of different cryoprotectants on the viability of Saanen goats embryos which were frozen and thawed with slow freezing method. The study was conducted on 15 Saanen goats and 3 bucks. Fluorogeston acetate (20 mg) impregnated intravaginal sponges were inserted in goats for 12 days regardless of the sexual cycle. Starting on the 9 th day of intravaginal sponge administration, follicle stimulating hormone (FSH) was injected intramuscularly, every 12 hours at decreasing doses (50-50; 30-30; 20-20 mg) for 3 days. Goats were mated naturally 24 hours after removal of the sponges. Embryos were recovered by laparotomic uterine flushing on the 7th day after the first mating. Collected embryos were frozen by using 3 different cryoprotectants [ethylene glycol (EG), glycerol, and dimetil sulfoksit DMSO)] with slow freezing technique. Thawed embryos were incubated at 38.5 ºC and 5% CO2. The viability of embryos was evaluated at the 24 th , the 48 th , the 72 nd hours after thawing. Superovulation response (≥4 Cl), embryo recovery rate and transferable embryo rate were found to be 93.3%, 72.3% and 58%, respectively. Viability rates of frozen and thawed embryos at the 24 th , the 48 th , the 72 nd hours were found respectively to be 64.86%; 56.76%; 54.05% in EG group, 54.55%, 45.45%; 36.36% in glycerol group and 46.88%; 40.63%; 28.13% in DMSO group. Viability rates of the frozen embryos with EG were statistically better than embryos frozen with glycerol and DMSO (P<0.05) at 72 nd hour. Survival rates of blastocysts frozen were 76%; 64%; 60%; 54.6% in EG group 45.5%; 36.4% in glycerol group, and 42.1%; 36.8%; 21.1% in DMSO group and at 72 nd hour the difference between EG and DMSO group was significant (P<0.05). In conclusion, viability of embryos at the 24 th , the 48 th , the 72 nd hours after thawing in EG group was significantly higher than the embryos frozen with other cryoprotectants.

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